Abstract
Two chitosanases produced byAspergillus fumigatus KB-1 were purified by ion exchange and size exclusion chromatographies. Molecular weights of chitosanases were 111.23 kDa (chito-sanase I) and 23.38 kDa (chitosanase II). TheN-terminal amino acid sequence of chitosanase II was determined as follows: YNLPNNLKQIYDKHKGKXSXVLAKGFTN. The optimum pH of the chitosanase I and II was 6.5 and 5.5, respectively. The optimum temperatures were 60°C for chitosanase land 70°C for chitosanase II. Hydrolysis products of two chitosanases were analyzed by HPLC and GPC. Chitosanase I hydrolyzed substrate to glucosamine. Chitosanase II produced chitooligosaccharides.
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Eom, T.K., Lee, K.M. Characteristics of chitosanases fromAspergillus fumigatus KB-1. Arch Pharm Res 26, 1036–1041 (2003). https://doi.org/10.1007/BF02994755
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DOI: https://doi.org/10.1007/BF02994755