Abstract
Temperature change is one of the major environmental factors that influence the human skin. However, the relationship between temperature and melanogenesis has received little attention. In the present study, we investigated the effects of temperature change on melanogenesis in a mouse melanocyte cell line (Mel-Ab), and primary cultured human melanocytes. We found that Mel-Ab cells cultured at low temperatures (31 and 34°C) produce less melanin than cells at 37°C. These results were confirmed by experiments upon human melanocytes, demonstrating that the hypopigmenting effect of low temperatures is not cell type dependent. The observed melanin production was found to be accompanied by tyrosinase activity at each temperature, indicating that tyrosinase activity is regulated by temperature. We further examined whether the incubation period at low temperatures plays an important role in the regulation of melanogenesis. Short exposures to 27°C for 1 h or 3 h did not affect tyrosinase activity or melanin synthesis, whereas long exposures to 31°C for 2 days or 6 days significantly reduced tyrosinase activity and melanin synthesis in a duration-dependent manner. Our results suggest that exposure to low temperature and the duration of this exposure are important regulators of melanogenesis.
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References
Alappatt, C., Johnson, C. A., Clay, K. L., and Travers, J. B., Acute keratinocyte damage stimulates platelet-activating factor production.Arch. Dermatol. Res., 292, 256–259 (2000).
Bentley, N. J., Eisen, T., and Goding, C. R., Melanocyte-specific expression of the human tyrosinase promoter: activation by the microphthalmia gene product and role of the initiator.Mol. Cell Biol., 14, 7996–8006 (1994).
Bowers, W., Blaha, M., Alkhyyat, A., Sankovich, J., Kohl, J., Wong, G., and Patterson, D., Artificial human skin: cytokine, prostaglandin, Hsp70 and histological responses to heat exposure.J. Dermatol. Sci., 20, 172–182 (1999).
Busca, R. and Ballotti, R., Cyclic AMP a key messenger in the regulation of skin pigmentation.Pigment. Cell Res., 13, 60–69 (2000).
Busca, R., Bertolotto, C, Ortonne, J. P., and Ballotti, R., Inhibition of the phosphatidylinositol 3-kinase/p70(S6)-kinase pathway induces B16 melanoma cell differentiation.J Biol. Chem., 271, 31824–31830 (1996).
Dooley, T. P., Gadwood, R. C., Kilgore, K. and Thomasco, L. M., Development of anin vitro primary screen for skin depigmentation and antimelanoma agents.Skin Pharmacol., 7, 188–200 (1994).
Eisinger, M. and Marko, O., Selective proliferation of normal human melanocytesin vitro in the presence of phorbol ester and cholera toxin.Proc. Natl. Acad. Sci. USA, 79, 2018–2022 (1982).
Englaro, W., Bertolotto, C., Busca, R., Brunet, A., Pages, G., Ortonne, J. P., and Ballotti, R., Inhibition of the mitogen-activated protein kinase pathway triggers B16 melanoma cell differentiation.J. Biol. Chem., 273, 9966–9970 (1998).
Gilchrest, B. A., Park, H. Y., Eller, M. S., and Yaar, M., Mechanisms of ultraviolet light-induced pigmentation.Photochem. Photobiol., 63, 1–10 (1996).
Hearing, V. J. and Jimenez, M., Analysis of mammalian pigmentation at the molecular level.Pigment. Cell Res., 2, 75–85 (1989).
Kim, D. S., Hwang, E. S., Lee, J. E., Kim, S. Y, Kwon, S. B., and Park, K. C., Sphingosine-1-phosphate Decreases Melanin Synthesis via Sustained ERK Activation and Subsequent MITF Degradation.J. Cell Sci., 116, 1699–1706 (2003).
Kim, D. S., Kim, S. Y, Chung, J. H., Kim, K. H., Eun, H. C., and Park, K. C., Delayed ERK activation by ceramide reduces melanin synthesis in human melanocytes.Cell Signal., 14, 779–785 (2002).
Mauderli, A. P., Vierck, C. J., Jr., Cannon, R. L, Rodrigues, A., and Shen, C, Relationships between skin temperature and temporal summation of heat and cold pain.J. Neurophysiol., 90, 100–109 (2003).
Medrano, E. E. and Nordlund, J. J., Successful culture of adult human melanocytes obtained from normal and vitiligo donors.J. Invest Dermatol., 95, 441–445 (1990).
Nakazawa, K., Sahuc, F., Damour, O., Collombel, C., and Nakazawa, H., Regulatory effects of heat on normal human melanocyte growth and melanogenesis: comparative study with UVB.J. Invest Dermatol., 110, 972–977 (1998).
Smalley, K. and Eisen, T., The involvement of p38 mitogenactivated protein kinase in the alpha-melanocyte stimulating hormone (alpha-MSH)-induced melanogenic and antiproliferative effects in B16 murine melanoma cells.FEBS Left., 476, 198–202 (2000).
Tachibana, M., MITF: a stream flowing for pigment cells.Pigment. Cell Res., 13, 230–240 (2000).
Tsuboi, T, Kondoh, H., Hiratsuka, J., and Mishima, Y., Enhanced melanogenesis induced by tyrosinase gene-transfer increases boron-uptake and killing effect of boron neutron capture therapy for amelanotic melanoma.Pigment. Cell Res., 11, 275–282 (1998).
Yanase, H., Ando, H., Horikawa, M., Watanabe, M., Mori, T., and Matsuda, N., Possible involvement of ERK 1/2 in UVA-induced melanogenesis in cultured normal human epidermal melanocytes.Pigment. Cell Res., 14, 103–109 (2001).
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Kim, DS., Park, SH., Kwon, SB. et al. Temperature regulates melanin synthesis in melanocytes. Arch Pharm Res 26, 840–845 (2003). https://doi.org/10.1007/BF02980030
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DOI: https://doi.org/10.1007/BF02980030