Abstract
Single-ascospore-derived isolates ofMycosphaerella fijiensis Morelet from false horn ‘Agbagba’ plantain leaves obtained from five different villages in southern Nigeria were stimulated to mate under artificial conditions. Pairs of isolates were incubated under blacklight on potato dextrose agar (PDA) with surface-sterilized plantain leaves or on PDA with autoclaved plantain leaves. Some isolates were observed to be sexually compatible by their ability to produce spherical to bulb-shaped fruiting body structures (FBS) and ascospores on pairing. FBS were observed to measure between 39–65 µm (smallest diameter) and 39–104 µm (largest diameter; mean 55.3×71.1 µm) in diameter, whereas ascospore lengths measured between 13.0 and 14.9 µm. Length of incubation time required for FBS production was dependent on the pair of isolates involved, the average being 40.1 days. With some pairs, ascospores were observed after 35 days of incubation.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Carlier, J., Fouré, E., Gauhl, F., Jones, D.R., Lepoivre, P., Mourichon, X.et al. (1999) Sigatoka leaf spots.in: Jones, D.R. [Ed.] Diseases of Banana, Abacá and Enset. CAB International, Wallingford, UK. pp. 37–92.
Carlier, J., Lebrun, M.H., Zapater, M.F., Dubois, C. and Mourichon, X. (1996) Genetic structure of the global population of black leaf streak fungus,Mycosphaerella fijiensis.Mol. Ecol. 5:499–510.
Carlier, J., Mourichon, X., González-de-León, D., Zapater, M.F. and Lebrun, M.H. (1994) DNA restriction fragment length polymorphisms inMycosphaerella species that cause banana leaf spot diseases.Mol. Plant Pathol. 84:751–756.
Fouré, E. (1985) Les cercosporioses du bananiers et leurs traitement. Etude de la sensibilité variétale des bananiers et plantain àMycosphaerella fijiensis Morelet au Gabon.Fruits 40:393–399.
Fullerton, R.A. and Olsen, T.L. (1991) Pathogenic variability inMycosphaerella fijiensis Morelet.in: Valmayor, R.V., Umali, B.E. and Bejosano, C.P. [Eds.] Banana Diseases in Asia and the Pacific. Proc. Technical Meeting on Diseases Affecting Banana and Plantain in Asia and the Pacific held at Brisbane, Australia, pp. 105–114. INIBAP, Montpellier, France.
Gauhl, F. (1989) Untersuchungen zur Epidemiologie und Ökologie der Schwarzen Sigatoka-Krankheit (Mycosphaerella fijiensis Morelet) an Kochbananen (Musa sp.) in Costa Rica.Göttinger Beitr. Land-Forstwirtsch. Tropen Subtropen vol. 42.
Mengistu, A., Rimmer, R.S. and Williams P.H. (1993) Protocols forin vitro sporulation, ascospore release, sexual mating, and fertility in crosses ofLeptosphaeria maculans.Plant Dis. 77:538–540.
Meredith, D.S. and Lawrence, J.S. (1969) Black leaf streak disease of bananas (Mycosphaerella fijiensis): Symptoms of disease in Hawaii, and notes on the conidial state of the causal fungus.Trans. Br. Mycol. Soc. 52:457–476.
Meredith, D.S., Lawrence, J.S. and Firman, I.D. (1973) Ascospore release and dispersal in black leaf streak disease of bananas(Mycosphaerella fijiensis).Trans. Br. Mycol. Soc. 60:547–554.
Mobambo, K.N., Gauhl, F., Vuylsteke, D., Ortiz, R., Pasberg-Gauhl, C. and Swennen, R. (1993) Yield loss in plantain from black Sigatoka leaf spot and field performance of resistant hybrids.Field Crops Res. 35:35–42.
Mourichon, X. and Zapater, M.F. (1990) Obtentionin vitro du stadeMycosphaerella fijiensis forme parfaite deCercospora fijiensis.Fruits 45:213–218.
Müller, R., Pasberg-Gauhl, C., Gauhl, F., Kaemmer, D. and Kahl, G. (1995) Tracing microsatellite polymorphism within the Nigerian population ofMycosphaerella fijiensis.Infomusa 4:9–11.
Müller, R., Pasberg-Gauhl, C., Gauhl, F., Ramser, J. and Kahl, G. (1997) Oligonucleotide fingerprinting detects genetic variability at different levels in NigerianMycosphaerella fijiensis.J. Phytopathol. 145:25–30.
Pasberg-Gauhl, C. (1989) Untersuchungen zur Symptomentwicklung und Bekämpfung der Schwarzen Sigatoka Krankheit (Mycosphaerella fijiensis Morelet) an Bananen (Musa spp.)in vitro und im Freiland.Göttinger Beitr. Land- Forstwirtsch. Tropen Subtropen vol. 40.
Romero, R. and Marín, D. (1990) Observation on the sensitivity of theMycosphaerella fijiensis monitoring method to triazole fungicides.in: Fullerton, R.A. and Stover, R.H. [Eds.] Sigatoka Leaf Spot Diseases of Bananas. Proc. International Workshop held at San José, Costa Rica (1989), pp. 100–106. INIBAP, Montpellier, France.
Simmonds, N.W. (1987) Classification and breeding of bananas.in: Persley, G.J. and De Langhe, E.A. [Eds.] Banana and Plantain Breeding Strategies. Proc. International Workshop held at Cairns, Australia. ACIAR Proceedings No. 21, pp. 78–83.
Slabaugh, W.R. (1990) New approaches for Chlorothalonil use in strategies to controlM. fijiensis.in: Fullerton, R.A. and Stover, R.H. [Eds.] Sigatoka Leaf Spot Diseases of Bananas. Proc. International Workshop held at San José, Costa Rica (1989), pp. 159–166. INIBAP, Montpellier, France.
Stover, R.H. (1963) Sexuality and heterothallism inMycosphaerella musicola.Can. J. Bot. 41:1531–1532.
Stover, R.H. (1980) Sigatoka leaf spot of bananas and plantains.Plant Dis. Rep. 64:750–756.
Stover, R.H. (1983) Effet du Cercospora noir sur les plantains en Amérique Centrale.Fruits 38:326–329.
Swennen, R. and Vuylsteke, D. (1993) Breeding black Sigatoka resistant plantains with a wild banana.Trop. Agric. 70:74–78.
Author information
Authors and Affiliations
Corresponding author
Additional information
http://www.phytoparasitica.org posting Dec. 16, 2002.
Rights and permissions
About this article
Cite this article
Etebu, E., Pasberg-Gauhl, C., Gauhl, F. et al. Preliminary studies ofin vitro stimulation of sexual mating among isolates ofMycosphaerella fijiensis, causal agent of black sigatoka disease in bananas and plantains. Phytoparasitica 31, 69–75 (2003). https://doi.org/10.1007/BF02979768
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02979768