Abstract
The water content of skin was determined using a FT near infrared (NIR) spectrometer. NIR diffuse reflectance spectra were collected from hairless mouse,in vitro, and from human inner arm,in vivo. It was found that the variation of NIR absorbance band 1450 nm from OH vibration of water and 1940 nm from the combination involving OH stretching and OH deformation, depending on the absolute water content of separated hairless mouse skin,in vitro, using the FT NIR spectrometer. Partial least squares regression (PLSR) was applied to develop a calibration model. The PLS model showed good correlation. For practical use of the evaluation of human skin moisture, the PLS model for human skin moisture was developedin vivo on the basis of the relative water content of stratum corneum from the conventional capacitance method. The PLS model predicted human skin moisture with a standard errors of prediction (SEP) of 3.98 at 1130-1830 nm range. These studies showed the possibility of a rapid and nondestructive skin moisture measurement using FT NIR spectrometer.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Caspers, P. J., Lucassen, G. W., Carter, E. A., Bruining, H. A., and Puppets, G. J.,In-vivo confocal raman microscopy of the skin: Non-invasive determination of molecular concentration profiles.J. Invest. Dermatol., 116, 434–442 (2001).
Courage W., Hardware and measuring principle: Corneometer, In Elsner, P., Berardesca, E., and Maibach, H. I. (Eds.). Bioengineering of the skin: Water and the Stratum Corneum. CRC press, Boca Raton, Chapter 14, (1994).
Egawa, M., Fukuhara, T., Takahashi, M., and Ozaki, Y., Determining Water Content in Human Nails with a Portable Near-Infrared Spectrometer.Appl. Spectrosc., 57, 473–478 (2003).
Heise, H. M., Clinical applications of near-and mid-infrared spectroscopy, In Gremlich, H. U., and Yan, B. (Eds.). Infrared and raman spectroscopy of biological materials. Marcel Dekker, New York, (2000).
Kumar, G. and Shmitt, J. M., Optimal probe geometry for near-infrared spectroscopy of biological tissue.Appl. Optics, 36, 2286–2293 (1997).
Libnau, F. O., Kvalheim, O. M., Christy, A. A., and Toft, J., Infrared spectra of water in the NIR and MIR region.Vib. Spectrosc., 7, 243–254 (1994).
Lucassen, G. W., Veen, G. N. A., and Jansen, J. A. J., Band analysis of hydrated human skin stratum corneum attenuated total reflectance fourier transform intrared spectrain vivo. J. Biomedical Optics, 3, 267–280 (1998).
Martin, K. A., Direct measurement of moisture in skin by NIR spectroscopy.J. Soc. Cosmet. Chem., 44, 249–261 (1993).
Martin, K.,In vivo measurements of water in skin by near-infrared reflectance.Appl. Spectrosc., 52, 1001–1007 (1998).
Martinsen, O. G., Grimnes, S., and Karlsen, J., Electrical methods for skin moisture assessment.Skin Pharmacol., 8, 237–245 (1995).
Obata, M. and Tagami, H., A rapidin vitro test to assess skin moisturizers.J. Soc. Cosmet. Chem., 41, 235–241 (1990).
Potts, R. O., Stratum corneum hydration: Experimental techniques and interpretations of results.J. Soc. Cosmet. Chem., 37, 9–33 (1986).
Prasch, T. H., Knübel, G., Schmidt-Fonk, K., Ortanderl, S., Nieveler, S., and Förster, T. H., Infrared spectroscopy of the skin: influencing the stratum corneum with cosmetic products.Int. J. Cos. Sci., 22, 371–383 (2000).
Rigal, J., Losch, M. J., Bazin, R., Camus, C., Sturelle, C., Descamps, V., and Leveque, J. L., Near-infrared spectroscopy: A new approach to the characterization of dry skin.J. Soc. Cosmet. Chem., 44, 197–209 (1993).
Salter, D. C., Examination of stratum corneum hydration state by electrical methods.Curr. Probl. Dermatol., 26, 38–47 (1998).
Sowa, M. G., Payette, J. R., and Mantsch, H. H., Near-infrared spectroscopic assessment of tissue hydration following surgery.J. Surg. Res., 86, 62–69 (1999).
Tagami, H., Quantitative measurements of water concentration of the stratumcorneum in vivo by high-frequency current.Acta Derm. Venerol. Suppl. (Stockh.), 185, 29–33 (1994).
Walling, P. L. and Dabney, J. M., Moisture in skin by near-infrared reflectance spectroscopy.J. Soc. Cosmet. Chem., 40, 151–171 (1989).
Wichrowski, K., Sore, G., and Khaïat, A., Use of infrared spectroscopy forin vivo measurement of the stratum corneum moisturization after application of cosmetic preparations.Int. J. Cos. Sci., 17, 1–11 (1995).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Suh, EJ., Woo, YA. & Kim, HJ. Determination of water content in skin by using a ft near infrared spectrometer. Arch Pharm Res 28, 458–462 (2005). https://doi.org/10.1007/BF02977676
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02977676