Abstract
Amperometric enzyme electrode for glucose is described based on the incorporation of glucose oxidase (GOD) into graphite paste modified with tetracyanoquinodimethane (TCNQ). The incorporated enzyme exhibits high activity and long-term stability over the earlier TCNQ-based glucose sensor (1). The sensor provides a linear response to glucose over a wide concentration range. The response time of the sensor is 15-50 sec, and the detection limit is 0.5 mM. Stable response to the substrate was obtained during a period of 35 d. Application of the sensor in the plasma analysis is reported.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Hendry, S. P. and Turner, A. P. F. (1988),Horm. Metab. Res. (Suppl.)20, 37.
Guilbault, G. G. (1984),Analytical Uses of Immobilized Enzymes, Dekker, New York.
Turner, A. P. F., Karube, I., and Wilson, G. S. (1987),Biosensors, Fundamentals and Applications, Oxford, New York.
Guilbault, G. G. and Kauffmann, J.-M. (1987),Biotechnol. Appl. Biochem. 9, 95.
Amine, A., Kauffmann, J.-M., and Patriarche, G. J. (1991),Talanta 38, 107.
Kawaguri, M., Yoshioka, T., and Nankai, S. (1990),Denki Kagaku 58, 1119.
Degani, Y. and Heller, A. (1987),J. Phys. Chem. 91, 1285.
Pandey, P. C., Tran-Minh, C., and Lantreibecq, F. (1991),Appl. Biochem. Biotech. 31(2), 145.
Albery, W. J., Bartlett, P. N., Cass, A. E. G., Craston, D. H., and Haggett, B. D. G. (1986),J. Chem. Soc. Faraday Trans. I 82, 1033.
Kulys, J. J. (1986),Biosensors 2, 3.
Cenas, N. K. and Kulys, J. J. (1981),Bioelectrochem. Bioenerg. 8, 103.
Cenas, N. K. and Kulys, J. J. (1981),J. Electroanal. Chem. 128, 103.
Hale, P. D., Inagaki, T., Karan, H. I., Okamoto, Y., and Skotheim, T. A. (1989),J. Am. Chem. Soc. 111, 3482.
Matuszewski, W. and Trojanowicz, M. (1988),Analyst 113, 735.
Wang, J. and Lin, M. S. (1989),Electroanalysis 1, 43.
Bonakdar, M., Vilchez, J. L., and Mottola, M. A. (1989),J. Electroanal. Chem. 266, 47.
Dicks, J. M., Aston, W. J., Davis, G., and Turner, A. P. F. (1986),Anal. Chim. Acta 182, 103.
Ikeda, T., Shibata, T., and Senda, M. (1989),J. Electroanal. Chem. 261, 351.
Barham, D. and Trinder, P. (1972),Analyst 97, 142.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pandey, P.C., Kayastha, A.M. & Pandey, V. Amperometric enzyme sensor for glucose based on graphite paste-modified electrodes. Appl Biochem Biotechnol 33, 139–144 (1992). https://doi.org/10.1007/BF02950782
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02950782