Abstract
Effect of plant growth regulators on morphogenesis was studied using callus derived from inflorescence segments of sugarcane cv. Co-91010 and CoC-671 cultured on MS medium supplemented with 2,4-D, malt extract, L-glutamine, casein hydroly sate and coconut water (CW). Comparison was made of different media combinations of CW (5,10%), kinetin (lmg/l), zeatin (0.5mg/l) andTDZ (0.5mg/l) to study callus growth and regeneration. Results showed that CW (5%) + zeatin (0.5mg/l) was more effective over other growth regulators for callus induction while CW 5% was found effective for the regeneration of healthy plants in cv. Co-91010. Different levels of proline and thioproline were tested on the induction of embryogenie callus and subsequent plant regeneration in var. CoC-671. Proline at 2.61M showed vigorous growth of embryogenie callus compared to thioproline. Plants regenerated were hardened and grown in the green house to maturity.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Afsharsterle, S., Pang, E.C.K., Brown, J. S. andKollmorgen, J.F. (1996). Embryogenie Callus Induction and Plant Regeneration in Triticum tauschii, the Diploid D-Genome Donor for Bread Wheat (Triticum aestivum).Australian J. Bot.44(4) 489–497.
Ahloowalia, B. S. andMaretzki, A. (1983). Plant regeneration via somatic embryogenesis in sugarcane.Plant CellRep.2:21–25.
Arencibia, A.D., Carmona, E.R., Tellez, P., Cahn, M-T., Yu, S. M., Trujilo, L. E. andOramas, P. (1998) An efficient protocol for sugarcane(Saccharum spp. L.) transformation mediated byAgrobacterium tumefaciens, Transgenic Research7, 213–222.
Beffagna, N., Pesd P. andRadice, M. (1986) Thioprolineinduced accumulation of proline in barley leaf sections.Plant Cell Environ.9: 141–146.
Blanco, M. D. A., Nieves, N., Sanchez, M., Borroto, C. G., Castillo, R., Gonzalez, J. L., Escalona, M., Baez, E. andHernandez, Z. (1997) Protein changes associated with plant regeneration in embryogenie calli of sugarcane (Saccharum sp.).Plant Cell Tiss. Organ Cult.51: 153–158.
Bower, R. andBirch, R. G. (1992) Transgenic sugarcane plants via microprojectile bombardment,The Plant J.2: 409–416.
Brisibe E.A., Miyake H., Taniguchi T. andMaeda E. (1994). Regulation of somatic embryogenesis in long-term callus cultures of sugarcane(paccharum officinarum)L.), New Phytol.126: 301–307.
Desai, N.S., Suprasanna, P. andBapat, V.A. (2004) A simple and reproducible method for direct somatic embryogenesis from immature inflorescence segments of sugarcane.Current Science87(6): 764–768.
Fitch, M. M. M. andMoore, P. H. (1990) Comparison of 2,4-D and picloram for selection of long-term totipotent green callus cultures of sugarcane.Plant Cell Tiss. Organ Cult.20:157–163.
Gallo-Meagher, M., English, R. G., Abouzid, A. (2000). Thidiazuron stimulates shoot regeneration of sugarcane embryogenie callus.In vitro Cell Dev. Biol. Plant.36: 37–40.
Ho, W. J. andVasil, I. K. (1983). Somatic embryogenesis in Sugarcane(Saccharum officinarum L.): Growth and plant regeneration from embryogenie cell suspension cultures.Ann. Bot.51: 719–726.
Irvine, J. E., Benda, G. T. A., Legendre, B. L., Machado, G. R., Jr (1991) The frequency of marker changes in sugarcane plants regenerated from callus culture. II. Evidence for vegetative and genetic transmission, epigenetic effects and chimeral disruption.Plant Cell Tiss. Organ Cult.26:115–125.
Liu, M. C. (1993) Factors affecting induction, somatic embryogenesis and plant regeneration of callus from cultured immature inflorescence of sugarcane.J. Plant Physiol.141: 714–720.
Milazzo, M. C., Zheng, Z., Gregory, K., Kendall, H. andShetty, K. (1999) Stimulation of benzyl-adenine -inducedin vitro shoot organogenesis and endogenous proline in melonCucumis melo L.) by fish protein hydrolysates in combination with proline analogs.Jour. Agr. Food Chem.47(4):1771–1775.
Murashige, T. andSkoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures.Physiol. Plant.15:473–497.
Murch, S. J., Victor J. M. R., Krishnaraj S. andSaxena P. K. (1999) The role of proline in thidiazuron-induced somatic embryogenesis in peanut.In Vitro Cell. Develop. Biol. Plant.35(1): 102–105.
Murthy, B. N. S., Murch, S. J. andSaxena, P. K. (1998). Thidiazuron: a potent cytokinin ofin vitro plant morphogenesis.In Vitro Cell Dev. Biol.34: 267–275.
Nakamura, C. andKeller, W. A. (1982). Plant regeneration from inflorescence cultures of hexaploid triticale.Plant Sci. Lett.24: 275–280.
Oropeza, M., andDe-Garcia, E. (1996) Somaclonal variants resistant to sugarcane mosaic virus and their agronomic characterization.In Vitro Cell. Develop. Biol. Plant32(1): 26–30.
Pareddy, D. R. andPetolino, I. F. (1990). Somatic embryogenesis and plant regeneration from immature inflorescences of several elite inbreds of maize.Plant Sci.67: 211–219.
Reddy, V. D., Suprasanna, P., Rao, K. V., Kavi Kishor, P. B. andReddy, G. M. (1991). Tissue culture studies in rice, maize and triticale.Indian Rev. of Life Sci.11: 29–52.
Rout, J. R. andLucas, W. J. (1996) Characterization and manipulation of embryogenie response fromin vitro cultured immature inflorescences of rice(Oryza sativa L.).Planta,198, 127–138.
Sahasrabudhe, N. A., Nandi, M., Bahulikar, R. A., Rao, P. S. andSuprasanna, P. (2000) A two-step approach to scale up green plant regeneration through somatic embryogenesis fromin vitro cultured immature inflorescence of a male sterile line and a maintainer line of rice.J. New Seed.2: 1–10.
Shetty, K. andMcKersie, B. D. (1993) Proline, thioproline and potassium mediated stimulation of somatic embryogenesis in alfalfa(Medicago sativa L.).Plant Sci.88:185–193.
Suprasanna, P., Rao, K. V. andReddy, G. M. (1997) Embryogenie callus in maize: genotypic and amino acid effects.Cereal Research Communication22 (1-2) : 79–82.
Taylor, P. W. J., Ko, H., Adkins, W., Rathus, C. andBirch, R. G. (1992). Establishment of embryogenie callus andhigh protoplast yielding suspension cultures of sugarcane(Saccharum spp.hybrids).Plant Cell Tiss. Organ Cult.28:69–78.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Suprasanna, P., Choudhary, R.S., Desai, N.S. et al. Regulation of somatic embryogenesis by plant growth regulators in sugarcane. Sugar Tech 7, 123–128 (2005). https://doi.org/10.1007/BF02950596
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02950596