Abstract
Analytical procedures for the determination of deoxynivalenol (DON) in bread and beer, using enzyme immunoassay (EIA) and HPLC methods, were developed. For determination of DON by EIA, aqueous raw extracts of bread or degassed beer were extracted by liquid-liquid partitioning with ethyl acetate, the organic solvent evaporated, and the residue redissolved in phosphate buffered saline (PBS) for analysis. For determination by HPLC (UV detection at 218 nm), DON in bread extracts or beer was purified on immunoaffinity chromatographic columns. In bread, detection limits for DON of 15 µg/kg (EIA) and 7 µg/kg (HPLC) were achieved, with mean recoveries of 81%. In beer, the detection limit for DON was 2 µg/l both in EIA and HPLC, with recoveries of 91–93%. Both methods showed good agreement of the results for naturally contaminated sample materials, with r2=0.993 for bread and r2=0.823 for beer, respectively.
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Curtui, V., Seidler, C., Dietrich, R. et al. Bestimmung von Deoxynivalenol in Brot und Bier. Mycotox Res 19, 144–148 (2003). https://doi.org/10.1007/BF02942954
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DOI: https://doi.org/10.1007/BF02942954