Abstract
Trehalose synthase gene (TSase) fromGrifola frondosa was transferred into sugarcane (Saccharum officinarum L.) usingAgrobacterium-mediated method to improve sugarcane drought-tolerance. The results indicated that embryogenic callus of sugarcane was sensitive toA. tumefaciens EHA105 strain in the transformation system employed. The high frequency of PPT-resistant plants were obtained from transformated with 3 weeks callus after incubation, which reached 4.5% on average. The transgenic plants was confirmed by PCR and southern blot analysis. Some transgenic plants showed multiple phenotypic alterations and some plants demonstrated improvement tolerance to osmotic stress.
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Wang, ZZ., Zhang, SZ., Yang, BP. et al. Trehalose synthase gene transfer mediated byAgrobacterium tumefaciens enhances resistance to osmotic stress in sugarcane. Sugar Tech 7, 49–54 (2005). https://doi.org/10.1007/BF02942417
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DOI: https://doi.org/10.1007/BF02942417