Abstract
TransgenicNicotiana tabacum cells were cultivated for the production of murine granulocyte macrophage-colony stimulating factor (mGM-CSF) in both a stirred, tank biore|actor and an airlift bioreactor with draft tube. Cell growth and mGM-CSF production in the airlift bioreactor were found to be better than those achieved in the stirred tank bioreactor. In the airlift bioreactor. 9.0 g/L of cells and 2.2 ng/mL of mGM-CSF were obtained (11.0 g/L and 2.4 ng/mL, respectively in shake flasks). Although the lag period was prolonged and mGM-CSF production was lowered by 33% in the stirred tank bioreactor as compared to the control culture, the maximum cell density was increased up to 12.0 g/L due to better mixing by agitation at the higher cell density.
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Lee, SY., Hur, W., Cho, G.H. et al. Cultivation of transgenicNicotiana tabacum suspension cells in bioreactors for the production of mGM-CSF. Biotechnol. Bioprocess Eng. 6, 72–74 (2001). https://doi.org/10.1007/BF02942254
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DOI: https://doi.org/10.1007/BF02942254