Abstract
We defined the role of the syrphid flyEristalis tenax in the survival and transmission of mycobacteria in pigs. The conditionally pathogenic mycobacterial (CPM) speciesMycobacterium chelonae was isolated from 10 % of liquid dung samples, and bothM. chelonae and another CPM speciesM. fortuitum were isolated from 7 (78 %) of the examinedE. tenax larvae collected from the same location. Mycobacteriosis of the lymph nodes of pigs from 3 infected farms was caused byM. avium subsp.avium, M. avium subsp.hominissuis, andM. fortuitum. M. avium subsp.avium andM. avium subsp.hominissuis of identical genotype and serotypes andM. fortuitum were isolated from 7 (1.9 %) larvae, 2 (7.4 %) puparia, and one (1.6 %) imago. The count of colony forming units isolated from larval skin covering (pouch) was higher (p ≤ 0.01) than that isolated from the internal organs of larvae. These results showed the potential forE. tenax larvae to spread mycobacteria throughout pig herds and the surrounding environment.
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Ayele W.Y., Macháčková M., Pavlík I.: The transmission and impact of paratuberculosis infection in domestic and wild ruminants.Vet.Med.Czech46, 205–224 (2001).
Beerwerth W., Kessel U.: Aviäre Mykobakterien im Kot von Wild-und Zoovögeln.Prax.Pneumol.30, 374–377 (1976a).
Beerwerth W., Kessel U.: Mykobakterien in der Umwelt von Mensch und Tier.Zbl.Bakt.Hyg.I.Abt.Orig.A235, 177–183 (1976b).
Beerwerth W., Schürmann J.: Zur Ökologie der Mykobakterien.Zbl.Bakt.Hyg.I.Abt.Orig.211, 58–69 (1969).
Beerwerth W., Eysing B., Kessel U.: Mykobakterien in Arthropoden verschiedener Biotope.Zbl.Bakt.Hyg.I.Abt.Orig.A244, 50–57 (1979).
Doležil Z.: Developmental stages of the tribeEristalini (Diptera, Syrphidae).Acta Entomol.Bohemoslov.69, 339–350 (1972).
Doležil Z., Rozkošný R.: Diptera Syrphidae, hover flies, pp. 347–362 in A.N. Nilsson (Ed.):Aquatic Insects of North Europe, Vol. 2. Apollo Books, Stenstrup (Denmark) 1997.
Dvorská L., Parmová I., Lávičková M., Bártl J., Vrbas V., Pavlík I.: Isolation ofRhodococcus equi and atypical mycobacteria from lymph nodes of pigs and cattle in herds with the occurrence of tuberculoid gross changes in the Czech Republic over the period 1996–1998.Vet.Med.Czech44, 321–330 (1999).
Dvorská L., Bartoš M., Ošťádal O., Kaustová J., Mátlová L., Pavlík I.: IS1311 and IS1245 restriction fragment length poly-morphism analyses, serotypes, and drug susceptibilities ofMycobacterium avium complex isolates obtained from a human immunodeficiency virus-negative patient.J.Clin.Microbiol.40, 3712–3719 (2002).
Fischer O.: The importance of diptera for transmission, spreading and survival of agents of some bacterial and fungal diseases in humans and animal. (In Czech)Vet.Med.Czech44, 133–160 (1999).
Fischer O., Mátlová L., Bártl J., Dvorská L., Melichárek I., Pavlík I.: Findings of mycobacteria in insectivores and small rodents.Folia Microbiol.45, 147–152 (2000).
Fischer O., Mátlová L., Dvorská L., Švástová P., Bártl J., Melichárek I., Weston R.T., Pavlík I.: Diptera as vectors of mycobacterial infections in cattle and pigs.Med.Vet.Entomol.15, 208–211 (2001).
Fischer O.A., Mátlová L., Bártl J., Dvorská L., švástová P., Du Maine R., Melichárek I., Bartoš M., Pavlík I.: Earthworms (Oligochaeta, Lumbricidae) and mycobacteria.Vet.Microbiol.91, 325–338 (2003a).
Fischer O.A., Mátlová L., Dvorská L., Švástová P., Pavlík I.: Larvae of the Oriental cockroach (Blatta orientalis), as passive vectors of causal agents of avian tuberculosis and paratuberculosis.Med.Vet.Entomol.17, 145–150 (2003b).
Fischer O.A., Mátlova L., Dvorska L., Švástova P., Bártl J., Weston R.T., Pavlik I.: BlowfliesCalliphora vicina andLucilia sericata as passive vectors ofMycobacterium aviúm subsp.avium, M. a. paratuberculosis andM. a. hominissuis.Med.Vet.Entomol.18, 116–122 (2004a).
Fischer O.A., Mátlová L., Dvorská L., Švástová P., Peral D.L., Weston R.T., Bartoš M., Pavlĺk I.: Beetles as possible vectors of infections caused byMycobacterium avium species.Vet.Microbiol.102, 247–255 (2004b).
Guerrero C., Bernasconi C., Burki D., Bodmer T., Telenti A.: A novel insertion element fromMycobacterium avium, IS1245, is a specific target for analysis of strain relatedness.J.Clin.Microbiol.33, 304–307 (1995).
Guizzardi F., Prestini A., Rossi L.: Infestation by larvaeEristalis tenax in cattle breed. (In Italian)Obiet.Doc.Vet.10, 27–30 (1989).
Jorgensen J.B.: Survival ofM. paratuberculosis in slurry.Nord.Vet.Med.29, 267–270 (1977).
Kazda J.:The Ecology of the Mycobacteria, 1st ed. Kluwer Academic Publishers, Dordrecht-Boston-London 2000.
Kunze Z.M., Portaels F., McFadden J.J.: Biologically distinct subtypes ofMycobacterium avium differ in possession of insertion sequence IS901.J.Clin.Microbiol.30, 2366–2372 (1992).
Lloyd J.B., Whittington R.J., Fitzgibbon C., Dobson R.: Presence ofMycobacterium avium subspeciesparatuberculosis in suspensions of ovine trichostrongylid larvae produced in fecal cultures artificially contaminated with the bacterium.Vet.Rec.148, 261–263 (2001).
Machačková M., Švastová P., Lamka J., Parmova I., Liška V., Šmolík J., Fischer O.A., Pavlík I.: Paratuberculosis in farmed and free-living wild ruminants in the Czech Republic (1999–2001).Vet.Microbiol.101, 225–234 (2004).
Mátlová L., Dvorská L., Bártl J., Bartoš J., Ayele W.Y., Alexa M., Pavlík, I.: Sources of mycobacterial infection in pig farms in the Czech Republic during the years 1996 to 2002.Vet.Med.Czech48, 343–357 (2003).
Mátlová L., Dvorská L., Dartoš M., Dočekal J., Trčková M., Pavlík I.: Tuberculous lesions in pig lymph nodes caused by kaolin fed as supplement.Vet.Med.Czech.49, 379–388 (2004a).
Mátlová L., Dvorská L., Paleček K., Maurenc L., Bartoš M., Pavlík I.: Impact of sawdust and wood shavings in bedding on pig tuberculous lesions in lymph nodes, and IS1245 RFLP analysis ofMycobacterium avium subsp.hominissuis of serotypes 6 and 8 isolated from pigs and environment.Vet.Microbiol.102, 227–236 (2004b).
Mátlová L., Dvorská L., Ayele W.Y., Bartoš M., Amemori T., Pavlík I.: Distribution ofMycobacterium avium complex isolates in tissue samples of pigs fed peat naturally contaminated with mycobacteria as a supplement.J.Clin.Microbiol.43, 1261–1268 (2005).
Matoušková O., Chalupa J., Cígler M., Hruška K.:Stat Plus — Manual. 1st ed. (In Czech) Veterinary Research Institute, Brno 1992.
Mijs W., De Haas P., Rossau R., Van der Laan T., Rigouts L., Portaels F., Van Soolingen D.: Molecular evidence to support a proposal to reserve the designationMycobacterium avium subsp.avium to bird-type isolates andM. avium subsp.hominissuis for the human/porcine type ofM. avium.Internat.J.Syst.Evol.Microbiol.52, 1505–1518 (2002).
Moucha J., Štys P.: Contribution to fauna of hover flies (Diptera, Syrphidae) in protected area Pavlov Hills in late summer. (In Czech)Ochrana Přirody11, 297–299 (1956).
Nagai R., Takewaki S., Wada A., Okuzumi K., Tobita A., Ohkubo A.: Development of rapid detection method for mycobacteria using PCR. (In Japanese)J.Med.Technol.38, 1247–1252 (1990).
Pavlík I., Mátlová L., Bártl J., Švástová P., Dvorská L., Whitlock R.: Parallel fecal and organMycobacterium avium subsp.paratuberculosis culture of different productivity types of cattle.Vet.Microbiol.77, 309–324 (2000a).
Pavlík I., Rozsypalová Z., Vesely T., Bartl J., Matlová L., Vrbas V., Valent L., Rajský D., Mraćko I., Hirko M., Miškovič P.: Control of paratuberculosis in five cattle farms by serological tests and fecal culture during the period 1990–1999.Vet.Med.Czech45, 61–70 (2000b).
Pavlík I., Mátlová L., Dvorská L., Bartl J., Oktábcová L., Dočkal J., Parmová I.: Tuberculous lesions in pigs in the Czech Republic during 1990–1999: occurrence, causal factors and economic loses.Vet.Med.Czech48, 113–125 (2003).
Povolný D.: Intestinal infections and flies. (In Czech)Vesmir53, 77–80 (1974).
Reichholf-Riehmová H.:Insect and Arachnid, 1st ed. (In Czech) Knižni Klub and Ikar Publishers, Prague 1997.
Richards W.D.: Effects of physical and chemical factors on the viability ofM. paratuberculosis.J.Clin.Microbiol.14, 587–588 (1981).
Ritacco V., Kremer K., Van der Laan T., Pijnenburg J.E.M., De Haas P.E.W., Van Soolingen D.: Use of IS901 and IS1245 in RFLP typing ofMycobacterium avium complex: relatedness among serovar reference strains, human and animal isolates.Internat.J.Tuberc.Lung Dis.2, 242–251 (1998).
Švastová P., Pavlík I., Bartoš M.: Rapid differentiation ofMycobacterium avium subsp.avium andMycobacterium avium subsp.paratuberculosis by amplification of insertion element IS901.Vet.Med.Czech47, 117–121 (2002).
Thorel M.F., Huchzermeyer H., Weiss R., Fontaine J.J.:Mycobacterium avium infections in animals. Literature review.Vet.Res.28, 439–447 (1997).
Trčková M., Mátlová L., Dvorská L., Pavlík I.: Kaolin, bentonite, and zeolites as feed supplements for animals: health advantages and risks.Vet.Med.Czech49, 389–399 (2004).
Trčková M., Mátlová L., Hudcová H., Faldyna M., Zralý Z., Dvorská L., Beran V., Pavlík I.: Peat as a feed supplement for animals: a review.Vet.Med.Czech50, 361–377 (2005).
Wayne L.G., Kubica G.P.: Family MycobacteriaceaeChester 1897, 63Al, in P.H.A Sneath, N.S. Mair, J.G. Holt (Eds):Bergey’s Manual of Systematic Bacteriology, Vol. 2. Williams & Wilkins, Baltimore 1986.
Whittington R.J., Marshall D.J., Nicholls P.J., Marsh I.B., Reddacliff L.A.: Survival and dormancy ofMycobacterium avium subsp.paratuberculosis in the environment.Appl.Environ.Microbiol.70, 2989–3004 (2004).
Wolinsky E., Schaefer W.B.: Proposed numbering scheme for mycobacterial serotypes by agglutination.Internat.J.Syst.Bacteriol.23, 182–183 (1973).
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The study was supported by grants NPV IB53009, QD1191, and MZE 0002716201 of theMinistry of Agriculture of the Czech Republic.
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Fischer, O.A., Mátlová, L., Dvorská, L. et al. Various stages in the life cycle of syrphid flies (Eristalis tenax; diptera: Syrphidae) as potential mechanical vectors of pathogens causing mycobacterial infections in pig herds. Folia Microbiol 51, 147–153 (2006). https://doi.org/10.1007/BF02932171
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DOI: https://doi.org/10.1007/BF02932171