Abstract
A chlorpyrifos (CPY)-degrading bacterial strain was isolated from an activated sludge sample collected from theDamascus Wastewater Treatment Plant, Syria. The isolation ofKlebsiella sp. was facilitated by the addition of CPY at a rate of 3.84 g/L of sludge weekly (selection pressure). Identification ofKlebsiella sp. was done using major staining and biochemical differentiation tests (Gram stain, cytochrome oxidase and some relevant saccharide fermentation tests using biochemical assays).Klebsiella sp. was maintained by culturing in a poor medium consisting of mineral salts and CPY as the sole carbon source. When 3 activated sludge samples were incubated in the presence of CPY (13.9 g/L sludge), 46 % of added CPY were degraded within 4 d. By comparison, within 4 d the isolatedKlebsiella sp. was found to break down 92 % of CPY when co-incubated in a poor mineral medium in which CPY was the sole carbon source (13.9 g/L poor medium). IsolatedKlebsiella sp. was able to tolerate up to 17.3 g of CPY in the poor medium.
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Abbreviations
- CPY:
-
chlorpyrifos [(O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)phosphothioate]
- TCP:
-
O-(3,5,6-trichloro-2-pyridyl)phosphothioate
- DWTP:
-
Damascus Wastewater Treatment Plant
- EC:
-
emulsifiable concentrate
- GC:
-
gas chromatography
- MSM:
-
mineral salt medium
References
Abacus Concepts. Statview 4.5 Statistical Program. Abacus Concepts Corporation, Berkeley (CA, USA) 1996.
Ajaz M., Jabeen N., Akhtar S., Rasool S.A.: Chlorpyrifos resistant bacteria from Pakistani soils: isolation, identification, resistance profile and growth kinetics.Pak.J.Bot.37, 381–388 (2005).
American Public Health Association (APHA):Standard Methods for the Examination of Water and Wastewater, 19th ed. American Public Health Association, New York 1995.
Boyd S.A., Shelton D.R.: Anaerobic biodegradation of chlorophenols in fresh and acclimated sludge.Appl.Environ.Microbiol.47, 272–277 (1984).
Cho C.M.-H., Mulchandani A., Chen W.: Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agent.Appl.Environ.Microbiol.68, 2026–2030 (2002).
Feng Y., Racke K.D., Bollag J.M.: Isolation and characterization of a chlorinated pyridinol-degrading bacterium.Appl.Environ.Microbiol.63, 4096–4098 (1997).
Hashimi I., Khan M.A., Jong-Guk K.: Malathion degradation byPseudomonas using activated sludge treatment system (biosimulator).Biotechnology3, 82–89 (2004).
Hayes W.J., Laws E.R.:Handbook of Pesticide Toxicology, Vol. 3. Classes of Pesticides. Academic Press, New York 1990.
Mallick B.K., Banerji A., Shakil N.A., Sethumathan N.N.: Bacterial degradation of chlorpyrifos in pure culture and in soil.Bull.Environ.Contam.Toxicol.62, 48–55 (1999).
Meister R.T.:Farm Chemicals Handbook ’92. Meister Publishing, Willoughby (USA) 1992.
Racke K.D., Laskowski A., Schultz M.R.: Resistance of chlorpyrifos to enhanced biodegradation in soil.J.Agric.Food Chem.38, 1430–1436 (1990).
Rani N.L., Lalitha D.-K.: Degradation of methyl parathion byPseudomonas putida.Can.J.Microbiol.4, 1000–1004 (1994).
Richards N.K., Boul H.L., Aislabie J.M.: Microbial degradation of DDT and its residue.New Zealand J.Agricul.Res.40, 269–282 (1997).
Richnis R., Kanaeva I., Mulchandani A., Chen W.: Biodegradation of organophosphorus pesticides using surface-expressed organophosphorus hydrolase.Nature Biotechnol.15, 984–987 (1997).
Singh B.K., Kuhad R.C., Singh A., Tripathi K.K., Ghosh P.K.: Microbial degradation of the pesticide lindane (γ-hexachlorocyclohexane).Adv.Appl.Microbiol.47, 269–298 (2000).
Singh B.K., Walker A., Morgan W.A., Wright D.J.: Effect of soil pH on the biodegradation of chlorpyrifos and isolation of chlorpyrifos-degrading bacterium.Appl.Environ.Microbiol.69, 5198–6206 (2003).
Singh B.K., Walker A., Morgan J.A.W., Wright D.J.: Biodegradation of chlorpyrifos byEnterobacter strain B-14 and its use in bioremediation of contaminated soils.Appl.Environ.Microbiol.70, 4855–4863 (2004).
US EPA: Information sheet: Cape cod golf course monitoring project. Office of Pesticide Programs, Washington (DC) 1986.
Wang L., Zhang L., Chen H., Tian Q., Zhu G.: Isolation of a triazophos-degrading strainKlebsiella sp. E6 effectively utilizing triazophos as a sole nitrogen source.FEMS Microbiol.Lett.253, 259–265 (2005).
Yang L., Zhao Y.H., Zhang B.X., Zhang X.: Isolation and characterization of a chlorpyrifos-degrading bacteria and its bioremediation application in soil.Wei Sheng Wu Xue Bao45, 905–909 (2005).
Yu Y.L., Fang H., Wang X., Wu X.M., Shan M., Yu J.Q.: Characterization of a fungal strain capable of degrading chlorpyrifos and its use in detoxification of the insecticide on vegetable.Biodegradation, in press (2006).
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Ghanem, I., Orfi, M. & Shamma, M. Biodegradation of chlorphyrifos byKlebsiella sp. isolated from an activated sludge sample of waste water treatment plant in damascus. Folia Microbiol 52, 423–427 (2007). https://doi.org/10.1007/BF02932098
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DOI: https://doi.org/10.1007/BF02932098