Abstract
Dye decolorization capacity of two white-rot fungi,Irpex lacteus andPhanerochaete chrysosporium, was compared in N-limited liquid cultures. The agitated cultures showed lower ability to decolorize azo dyes Reactive Orange 16 and Naphthol Blue Black than static cultures. Similar effect was also observed with other structurally different synthetic dyes. The effect of surfactants on the decolorization process is discussed. A significant increase in the Reactive Orange 16 decolorization by the agitatedI. lacteus cultures was observed after adding 0.1% Tween 80, following a higher Mn-dependent peroxidase production. Thein vitro dye decolorization using the purified enzyme proved its decolorization ability.
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Abbreviations
- BPB:
-
Bromophenol Blue
- CSB:
-
Chicago Sky Blue
- CuP:
-
copper(II) phthalocyanine
- DB3:
-
Disperse Blue 3
- DMAB:
-
dimethylamine-borane
- LiP:
-
lignin peroxidases
- MB:
-
Methylene Blue
- MBTH:
-
3-methyl-2-benzothiazolinone hydrazone
- MnP:
-
Mn-dependent peroxidases
- NBB:
-
Naphthol Blue Black
- RBBR:
-
Remazol Brilliant Blue R
- RO16:
-
Reactive Orange 16
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The work was supported by the projects no. IAA 602 0411 of theGrant Agency of the Academy of Sciences of the Czech Republic, COST no. 1P 04O C8 47.002 and by theInstitutional Research Concept no. AV 0Z 5020 0510.
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Svobodová, K., Erbanová, P., Sklenář, J. et al. The role of Mn-dependent peroxidase in dye decolorization by static and agitated cultures ofIrpex lacteus . Folia Microbiol 51, 573–578 (2006). https://doi.org/10.1007/BF02931622
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DOI: https://doi.org/10.1007/BF02931622