Abstract
Transition from exponential phase of growth to stationary phase inStreptomyces aureofaciens is characterized by a decrease in the rate of translation and induction of tetracycline (Ttc) biosynthesis. In exponential phase, no significant changes were found in the activity of ribosomes at binding of ternary complex Phe-tRNA.EF-Tu.GTP to the A-site on ribosomes. Overexpression of Ttc in stationary phase is accompanied by a decrease in the binding of the ternary complex Phe-tRNA.EF-Tu.GTP to the A-site of ribosome and a formation of an aggregate with Ttc by part of the ribosomes. Antibiotics that cause ribosome to stall or pause could increase the requirement for tmRNA in the process calledtrans-translation. We found differences in the level of tmRNA during the development ofS. aureofaciens. Subinhibitory concentrations of Ttc, streptomycin and chloramphenicol induced an increase in the tmRNA level in cells from the exponential phase of growth.In vitro trans-translation system ofS. aureofaciens was sensitive to Ttc at a concentration of >15 µmol/L; thetrans-translation system can thus be considered to contribute to resistance against Ttc produced only at sublethal concentrations. These experiments suggest that the main role of the rising tmRNA level at the beginning of the Ttc production is connected with ribosome rescue.
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Abbreviations
- Clm:
-
chloramphenicol
- Kir:
-
kirromycin
- Stm:
-
streptomycin
- Ttc:
-
tetracycline
- DTT:
-
1,4-dithiothreitol
- tmRNA:
-
transfer-messenger RNA
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This work was supported by grant of theGrant Agency of the Czech Republic no. 203/05/0106 to the corresponding author.
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Palečková, P., Bobek, J., Felsberg, J. et al. Activity of translation system and abundance of tmRNA during development ofStreptomyces aureofaciens producing tetracycline. Folia Microbiol 51, 517–524 (2006). https://doi.org/10.1007/BF02931615
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DOI: https://doi.org/10.1007/BF02931615