Abstract
TheAgrobacterium tumefaciens-mediated transformation of the zygomycetous fungusMucor circinelloides is described. A method was also developed for the hygromycin B-based selection ofMucor transformants. Transformation with the hygromycin B phosphotransferase gene ofEscherichia coli controlled by the heterologousAspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase chain reaction and Southern hybridization: the latter analyses revealed integrations in the host genome at different sites in different transformants. The stability of transformants remained questionable during the latter analyses.
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This research was supported in part by theHungarian Scientific Research Fund (OTKA) grants T037471, F046658 and D048537.
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Nyilasi, I., Ács, K., Papp, T. et al. Agrobacterium tumefaciens-mediated transformation ofMucor circinelloides . Folia Microbiol 50, 415–420 (2005). https://doi.org/10.1007/BF02931423
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DOI: https://doi.org/10.1007/BF02931423