Abstract
Using the polymerase chain reaction (PCR),Toxoplasma gondii from gene TGR 1E with primers TGR1E-1, TGR1E-2 (standard PCR), and from B1 gene with primers TM1, TM2, TM3 (hemi-nested PCR) was detected in biological samples from 347 individuals (441 biological materials). Of the total of 441 biological materials,T. gondii DNA was detected in 5.2 %; it was positive in the following samples: blood (n=6), blood from newborns (2), biopsies (2) and samples of progenitor cells (2) (from candidates for bone marrow transplantation). DNA ofT. gondii was also revealed in 11 samples (8.3 %) of 120 cases of pregnant women during prenatal examinations. A positive result in the blood was also found in two cases of newborn babies from mothers who were infected in later pregnancy. The positive PCR examination was confirmed by serological methods (ELISA and complement fixation test). Agreement of PCR results and the detection of antibodies against toxoplasma was found in 83.3 %. Rapid PCR examination for the confirmation of acute parasitemiaT. gondii is particularly important for the patients in whom the infection may cause serious consequences (e.g., for fetus in pregnant women or for patients suffering from imunosuppression).
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Belanger F., Derouin F., Grangeot-Keros L., Mayer L.: Incidence and risk factors of toxoplasmosis in a cohort of human immunodeficiency virus-infected patients: 1988–1995.Clin.Inf.Dis. 28, 575–581 (1999).
Burg L.J., Grover M.C., Pouletty P., Boothroyd C.J.: Direct and sensitive detection of a pathogenic protozoanToxoplasma gondii by polymerase chain reaction.J.Clin.Microbiol. 27, 1787–1792 (1989).
Čatár G., Červeň D., Jalili N.:Toxoplasma gondii.Bratisl.Lek.Listy 99, 579–583 (1998).
Graco P., Vimercati A., Angelici C.M., Carbonara S., Doria G.: Toxoplasmosis in pregnancy is still an open subject.J.Perinat.Med. 31, 36–40 (2003).
Gratzl R., Hayde M., Kohlhauser C., Hermon M., Burda G.: Follow-up of infants with congenital toxoplasmosis detected by polymcrase chain reaction analysis of amniotic fluid.Eur.J.Clin.Microbiol.Infect.Dis. 17, 853–858 (1998).
Gross U., Roggenkamp A., Janitschke K., Heesemann J.: Improved sensitivity of the polymerase chain reaction for detection ofToxoplasma gondii biological and human clinical specimens.Eur.J.Clin.Microbiol.Infect.Dis. 11, 33–39 (1992).
Guy C.E., Joynson M.H.D.: Potential of the polymerase chain reaction in the diagnosis of activeToxoplasma infection by detection of parasite in blood.J.Infect.Dis. 172, 319–322 (1995).
Guy C.E., Pelloux H., Lappalainen M., Aspöck H., Hassl A.: Interlaboratory comparison of polymerase chain reaction for the detection ofToxoplasma gondii DNA added to samples of amniotic fluid.Eur.J.Clin.Microbiol.Infect.Dis. 15, 836–839 (1996).
Hohlfeld P., Daffos F., Costa M.J., Thulliez P., Forestier F.: Prenatal diagnosis of congenital toxoplasmosis with a polymerasechain reaction test on amniotic fluid.N.Engl.J.Med. 331, 695–699 (1994).
Jenum A.P., Pedersen S.B., Melby K.K., Kapperund G., Whitelaw A.: Incidence ofToxoplasma gondii infection in 35,940 pregnant women in Norway and pregnancy outcome for infected women.J.Clin.Microbiol. 36, 2900–2906 (1998).
Kodym P., Tolarová V.: Laboratory diagnosis of toxoplasmosis. (In Czech)Remedia Klin.Mikrobiol. 2, 224–226 (1998).
Lamoril J., Molina M.J., Gouvello A., Garin J.Y., Deybach C.J.: Detection by PCR ofToxoplasma gondii in blood in the diagnosis of cerebral toxoplasmosis in patients with AIDS.J.Clin.Pathol. 49, 89–92 (1996).
Palička P., Slabá H., Zitek K.: Congenital toxoplasmosis in population. (In Czech)Prakt.Gynekol. 5, 23–27 (1998).
Pelloux H., Wiess J., Simon J., Muet F., Fricker-Hidalgo H.: A new set of primers for the detection ofToxoplasma gondii in amniotic fluid using polymerase chain reaction.FEMS Microbiol.Lett. 138, 11–15 (1996).
Pujol-Riqué M., Derouin F., García-Quintanilla A., Valls E.M., Miro J.M.: Design of a one-tube hemi-nested PCR for detection ofToxoplasma gondii and comparison of three DNA purification methods.J.Med.Microbiol. 48, 857–862 (1999).
Slavin M.A., Mayers J.D., Remington J.S., Hackman R.C.:Toxoplasma gondii infection in marrow transplantant recipients: a 20 year experience.Bone Marrow Transplant. 13, 549–557 (1994).
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Čermáková, Z., Ryšková, O. & Plíšková, L. Polymerase chain reaction for detection ofToxoplasma gondii in human biological samples. Folia Microbiol 50, 341–344 (2005). https://doi.org/10.1007/BF02931415
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DOI: https://doi.org/10.1007/BF02931415