Abstract
The effect of ultraviolet-B radiation (UV-B; 280–320 nm) on induction of nitric oxide was estimated in the suspensions of green algaChlorella pyrenoidosa with or without the NO scavengerN-acetyl-l-cysteine, and reductants such as 1,4-dithiothreitol, glutathione (reduced form), and ascorbic acid. Exogenously added sodium nitroprusside (NO donor), glutathione, 1,4-dithiothreitol, and ascorbic acid were able to prevent chlorophyll loss mediated by UV-B. Addition of NO to algal suspensions irradiated by UV-B increased the activity of catalase and superoxide dismutase but lowered the activity of phenylalanine ammonia-lyase. UV-B thus appears to be a strong inducer of NO production, exogenously added NO and reductants protecting the green alga against UV-B-induced oxidative damage.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Aebi M.: Catalasein vitro.Meth.Enzymol. 105, 121–126 (1984).
Alvarez M.E., Pennell R.I., Meijer P.J., Ishikawa A., Dixon R.A., Lamb C.: Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity.Cell 92, 773–784 (1998).
Beligni M.V., LaMattina L.: Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues.Planta 208, 337–314 (1999).
Bischoff H.W., Bold H.C.: Phycological studies — IV. Some algae from enchanted rock and related algal species.The University of Texax Publication, pp. 99–105 (1963).
Bradford M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding.Anal.Biochem. 72, 248–254 (1976).
Chow W.S, Strid A., Anderson J.M.: Short-term treatment of pea plants with supplementary UV-B: recovery time course of some photosynthetic functions and components, pp. 361–364 in I.V. Murata (Ed.):Research in Photosynthesis, Vol. IV. Kluwer Academic Publishers, Dordrecht (The Netherlands) (1992).
Danilov R.A., Ekelund N.G.A.: Effects of increasing doses of UV-B radiation on photosynthesis and motility inChlamydomonas reinhardtii.Folia Microbiol. 45, 41–44 (2000).
Delledonne M., Xia Y., Dixon R.A., Lamb C.: Nitric oxide functions as a signal in plant disease resistance.Nature 394, 585–589 (1998).
Durner J., Wendehenne D., Klessig D.F.: Defense gene induction in tobacco by nitric oxide, cyclic GMP and cyclic ADP ribose.Proc.Nat.Acad.Sci.USA 95, 10328–10333 (1998).
Guittet O., Roy B., Leporre M.: Nitric oxide: a radical molecule in quest of free radicals in proteins.Cell Mol.Life Sci. 55, 1054–1067 (1999).
Jeanine L.A.W., Bruce M.G., Ralph E.H.S.: Ultraviolet radiation effects on a microscopic green alga and the protective effects of natural dissolved organic matter.Photochem.Photobiol. 69, 536–544 (1999).
Klepper L.: NO X evolution by soybean leaves treated with salicylic acid and selected derivatives.Pestic.Biochem.Physiol. 39, 43–48 (1991).
Koutný M.: From no-confidence to nitric oxide acknowledgement: a story of bacterial nitric-oxide reductase.Folia Microbiol. 45 197–203 (2000).
Mackerness S., John C.F., Jordan B., Thomas B.: Early signaling components in ultraviolet-B responses: distinct roles for different reactive oxygen species nitric oxide.FEBS Lett. 48, 237–242 (2001).
Majumdar S., Gupta R., Dogra N.: Interferon-γ- and lipopolysaccharide-induced tumor necrosis factor-α is required for nitric oxide production: tumor necrosis factor-α and nitric oxide are independently involved in the killing ofMycobacterium microti in interferon-γ- and lipopolysaccharide-treated J774A.1 cells.Folia Microbiol. 45, 457–464 (2000).
Mallick N., Mohn F.H., Rai L.C., Soeder C.T.: Evidence for the non-involvement of nitric oxide synthase in nitric oxide production by the green algaScenedesmus obliquus.J.Plant Physiol. 156, 423–426 (2000a).
Mallick N., Rupama, Mohn F.H., Rai L., Soeder C.J.: Impact of physiological stresses on nitric oxide formation by green algaScenedesmus obliquus.J.Microbiol.Biotechnol. 10, 300–306 (2000b).
Mittal J., Dogra N., Dass R., Majumdar S.:In Vitro Effects of cAMP-elevating agents and glucocorticoid either alone or in combination on the production of nitric oxide, interleukin-12 and interleukin-10 in IFN-γ- and LPS-activated mouse peritoneal macrophages.Folia Microbiol. 47, 709–716 (2002).
Mittal J., Dogra N., Vohra H., Majumdar S.: Effects of prostaglandin E2 and nitric oxide inhibitors on the expression of interleukin-10, interleukin-12 and MHC class-II molecules inMycobacterium microti-infected and interferon-γ-treated mouse peritoneal macrophages.Folia Microbiol. 46, 259–264 (2001).
Murphy M., Noack E.: Nitric oxide assay using hemoglobin method.Meth.Enzymol. 233, 241–250 (1994).
Navarre D.A., Wendehenne D., Durner J., Noad R., Klessig D.F.: Nitric oxide modulates the activity of tobacco aconitase.Plant Physiol. 122, 573–582 (2000).
Pennell R.I., Lamb C.: Programmed cell death in plants.Plant Cell 9, 1157–1168 (1997).
Schreck R., Bacuerle P.A.: A role of oxygen radicals as second messengers.Trends Cell Biol. 11, 39–42 (1991).
Sigler K., Chaloupka J., Brozmanová J., Stadler N., Höfer M.: Oxidative stress in microorganisms — I. Microbialvs. higher cells damage and defenses in relation to cell aging and death.Folia Microbiol. 44, 587–624 (1999).
Stamler J.S.: Redox signaling: nitrosylation and related target interactions of nitric oxide.Cell 78, 931–936 (1994).
Trebichavsky I., Zídek Z., Franková D., Zahradníčková M., Šplíchal I.: Nitric oxide metabolites in gnotobiotic piglets orally infected withSalmonella enterica serovar Typhimurium.Folia Microbiol. 46, 353–358 (2001).
Yamasaki H., Sakihama Y.: Simultaneous production of nitric oxide and peroxynitrite by plant nitrate reductase,in vitro evidence for the NR-dependent formation of active nitrogen species.FEBS Lett. 468, 89–93 (2000).
Zhou G.L., Gui X.F., Zhong X.L., Zhu R.P.: A method of measuring SOD enzyme activity. (In Chinese)Progr.Biochem.Biophys. 4, 71–73 (1986).
Zimmermann A., Hahlbrock K.: Light-induced changes of enzyme activities in parsley cell suspension cultures. Purification and some properties of phenylalanine ammonia-lyase (EC 4.3.1.5).Arch.Biochem.Biophys. 166, 54–62 (1975).
Author information
Authors and Affiliations
Corresponding authors
Additional information
This work was supported by grant no. 30170186 fromChinese Natural Science Fund awarded to Prof. X. Wang.
Rights and permissions
About this article
Cite this article
Chen, K., Feng, H., Zhang, M. et al. Nitric oxide alleviates oxidative damage in the green algaChlorella pyrenoidosa caused by UV-B radiation. Folia Microbiol 48, 389–393 (2003). https://doi.org/10.1007/BF02931372
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02931372