Abstract
The viability of 250 basidiomycete strains was determined after a 2-d and then after a 2-year storage under liquid nitrogen using two different freezing protocols. Using an original agar plug protocol (OP), 162 strains (65%) of the 250 strains survived a 2-d storage and 158 strains (63 %) survived a 2-year storage in liquid nitrogen. Using a straw protocol (CP), 246 strains (98 %) of the 250 strains survived a 2-d storage and 243 strains (97 %) a 2-year storage in liquid nitrogen. In addition, other 106 strains were newly estimated using the CP protocol; 104 (98 %) of them survived successfully a 2-d storage and 101 (95 %) of them survived a 2-year storage in liquid nitrogen. The results indicate that the protocol used for cryopreservation can significantly influence strain survival. Markedly better results were obtained using the CP protocol.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Alexander M., Daggett P.-M., Gherna R., Jong S.C., Simione F., Hatt H.:American Type Culture Collection Methods I. Laboratory Manual on Preservation Freezing and Freeze Drying. American Type Culture Collection, Rockville (USA) 1980.
Butterfield W., Jong S.C., Alexander M.T.: Polypropylene vials for preserving fungi in liquid nitrogen.Mycologia70, 1122–1124 (1978).
Challen M.P., Elliott T.J.: Polypropylene straw ampoules for the storage of microorganisms in liquid nitrogen.J.Microbiol.Meth.5, 11–23 (1986).
Chvostová V., Nerud F., Homolka L.: Viability of wood-inhabiting basidiomycetes following cryogenic preservation.Folia Microbiol.40, 193–197 (1995).
Elliott T.J.: Alternative ampoule for storing fungal cultures in liquid nitrogen.Trans.Brit.Mycol.Soc.67, 545–546 (1976).
Heckley R.J.: Preservation of microorganisms.Adv.Appl.Microbiol.24, 1–53 (1978).
Hoffmann P.: Cryopreservation of fungi.World J.Microbiol.Biotechnol.7, 92–94 (1991).
Homolka L.: On the problem of maintenance and cultivation of higher fungi.Folia Microbiol.21, 189–190 (1976).
Homolka L., Lisá L., Eichlerova I., Nerud F.: Cryopreservation of some basidiomycete strains using perlite.J.Microbiol.Meth.47, 307–313 (2001).
Hubálek Z.:Cryopreservation of Microorganisms. Academia, Prague 1996.
Hwang S.-W.: Investigation of ultra-low temperature for fungal cultures — I. An evaluation of liquid nitrogen storage for preservation of selected fungal cultures.Mycologia60, 613–621 (1968).
Hwang S.-W., Kwolek W.F., Haynes W.C.: Investigation of ultra-low temperature for fungal cultures — III. Viability and growth rate of mycelial cultures following cryogenic storage.Mycologia68, 377–387 (1976).
Jong S.C.: Conservation of the cultures, pp. 119–135 inThe Biology and Cultivation of Edible Mushrooms (S.S. Chang, W.A. Hay, Eds). Academic Press, New York 1978.
Jong S.C., Atkins W.B.: Conservation, collection and distribution of cultures, pp. 153–194 inFungi Pathogenic for Humans and Animals. Part B, Pathogenicity and Detection II (H.D.H. Howard, Ed.). Marcel Dekker, New York 1985.
Jong S.C., Davis E.E.: Germplasm preservation of edible fungi in culture through cryogenic storage, pp. 213–225 inProc. Internat. Symp. Scientific and Technical Aspects of Cultivating Edible Fungi. The Penna State University, University Park (USA) 1986.
Morris G.J., Smith D., Coulson G.E.: A comparative of the changes in the morphology of hyphae during freezing and viability upon thawing for twenty species of fungi.J.Gen.Microbiol.134, 2897–2906 (1988).
Niku-Paavola M.L., Raaska L., Itavaara M.: Detection of white-rot fungi by a non-toxic stain.Mycol.Res.94, 27–31 (1990).
Prescott J.M., Kernkamp M.F.: Genetic stability ofPuccinia graminis f.sp.tritici in cryogenic storage.Plant Dis.Rep.55, 695–696 (1971).
Smith D.: Liquid nitrogen storage of fungi.Trans.Brit.Mycol.Soc.79, 415–421 (1982).
Smith D.: The use of cryopreservation in theex-situ conservation of fungi.Cryo-Letters19, 79–90 (1998).
Smith D., Ward S.M.:Notes on the Preservation of Fungi. CAB International Mycological Institute, Kew (UK) 1987.
Stalpers J.A., de Hoog A., Vlug I.J.: Improvement of the straw technique for the preservation of fungi in liquid nitrogen.Mycologia79, 82–89 (1987).
Stoychev I., Homolka L., Nerud F., Lisá L.: Activities of ligninolytic enzymes in some white-rot basidiomycete strains after recovering from cryopreservation in liquid nitrogen.Antonie van Leeuwenhoek73, 211–214 (1998).
Withers L.A.: Low temperature storage of plant tissue.Adv.Biochem.Eng.18, 102–150 (1980).
Author information
Authors and Affiliations
Corresponding author
Additional information
This work was supported byInstitutional Research Concept no. AV 0Z 502 0903.
Rights and permissions
About this article
Cite this article
Homolka, L., Lisá, L. & Nerud, F. Viability of basidiomycete strains after cryopreservation: Comparison of two different freezing protocols. Folia Microbiol 48, 219–226 (2003). https://doi.org/10.1007/BF02930959
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02930959