Abstract
Effects of major physicochemical and structural parameters of cellulose on the rate and degree of its enzymatic hydrolysis were tested with cellulosic materials from various sources. Some different pretreatments were: mechanical (milling), physical (X-ray irradiation), and chemical (cadoxen, H3PO4, H2SO4, NaOH, Fe2+/H2O2). The average size of cellulose particles and its degree of polymerization had little effect on the efficiency of enzymatic hydrolysis. For samples of pure cellulose (cotton linter, microcrystalline cellulose, α-cellulose), increase in the specific surface area accessible to protein molecules and decrease in the crystallinity index accelerated the enzymatic hydrolysis (the correlation coefficients were 0.89 and 0.92, respectively). In the case of lignocellulose (bagasse), a quantitative linear relationship only between specific surface area and reactivity was observed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bikales, N. M. and Segal, L., eds. (1971),Cellulose and Cellulose Derivatives, vol. 1 and 2, Wiley Intersci., New York.
Rogovin, Z. A. (1971),Vysokotnolekulyarniye Soedineniya 2, 1586.
Atalla, R. H., ed. (1987),The Structure of Cellulose. Characterization of the Solid State, ACS Symp. Ser., Washington, DC. 58.
Tripp, V. W. (1971),Cellulose and Cellulose Derivatives, vol. 1, Bikales, N. M. and Segal, L., eds., Wiley Intersci., New York, pp. 214–234.
Focher, B., Marzetti, A., Sarto, V., Beltrame, P. L., and Carniti, P. (1984),J. Appl. Polym. Sci. 29, 3329.
Fan, L. T., Lee, Y.-H., and Beardmore, D. H. (1980),Biotechnol. Bioeng. 22, 177.
Ryu, D. D. Y. and Mandels, M. (1980),Enzyme Microb. Technol. 2, 91.
Ryu, D. D. Y., Lee, S. B., Tassinari, T., and Macy, C. (1982),Biotechnol. Bioeng. 24, 1047.
Tanaka, M., Taniguchi, M., Morita, T., Matsuno, R., and Kamikubo, T. (1979),J. Ferment. Technol. 57, 117.
Sasaki, T., Tanaka, T., Nanbu, N., Sato, Y., and Kainuma, K. (1979),Biotechnol. Bioeng. 21, 1031.
Welmer, P. J. and Weston, W. M. (1985),Biotechnol. Bioeng. 27, 1540.
Cowling, R. B. (1975),Biotechnol. Bioeng. Symp. 5, 163.
Chernoglazov, V. M., Klyosov, A. A., and Ermolova, O. V. (1983),Biokhimiya 48, 1617.
Lee, S. B., Kim, I. H., Ruy, D. D. Y., and Taguchi, H. (1983),Biotechnol. Bioeng. 25, 33.
Gharpuray, M., Lee, Y.-H., and Fan, L. T. (1983),Biotechnol. Bioeng. 25, 157.
Knappert, D., Grethlein, H., and Converse, A. (1978),Proceedings of IV Joint US/USSR Enzyme Engineering Conference, New Orleans, Lousiana, pp. 403–419.
Puri, V. P. (1984),Biotechnol. Bioeng. 26, 1219.
Shewale, I. G. and Sadana, J. C. (1979),Can. J. Microbiol. 25, 773.
Rivers, D. B. and Emert, G. H. (1988),Biotechnol. Bioeng. 31, 278.
Stone, J., Scallan, A., Donefer, E., and Ahlgren, E. (1969),Adv. Chem. Ser. 95, 219.
Lin, K. W., Ladisch, M. R., Voloch, M., Patterson, J. A., and Noller, C. H. (1985),Biotechnol. Bioeng. 27, 1427.
Burns, D. S., Ooshima, H., and Converse, A. O. (1989),Appl. Biochem. Biotechnol. 20/21, 79.
Mandels, M., Andreotti, R., and Roche, C. (1976),Biotechnol. Bioeng. Symp. 6, 21.
Klyosov, A. A., Rabinowitch, M. L., Sinitsyn, A. P., Churilova, I. V., and Grigorash, S. Yu. (1980),Bioorgan. Khim. 6, 1225.
Sinitsyn, A. P. and Klyosov, A. A. (1981),Prikl. Biokhim. Mikrobiol. 17, 683.
Kovalev, G. V., Sinitsyn, A. P., Volf, E. G., Kalyazin, E. P., Bugaenko, L. T., and Klyosov, A. A. (1987),British Polymer J. 19, 63.
Rogovin, Z. A. (1972),Chemistry of Cellulose (in Russian), Khimiya, Moscow.
Bolotnikova, L. S., Danilov, V. S., and Samsonova, I. A. (1966),J. Prikl. Khim. 39, 176.
Bolotnikova, L. S. and Samsonova, I. A. (1964),Vysokomolekulyamiye Soedineniya 6, 533.
Segal, L., Creely, J. J., Martin, A. E., and Conrad, C. M. (1959),Text. Res. J. 29, 786.
Lebedeva, O. A., Ugarova, N. N., and Berezin, I. V. (1977),Biokhimiya 42, 1372.
Berezin, I. V., Rabinowitch, M. L., and Sinitsyn, A. P. (1977),Biokhimiya 42, 1631.
Gusakov, A. V., Sinitsyn, A. P., and Klyosov, A. A. (1987),Biotechnol. Bioeng. 29, 906.
Klyosov, A. A. and Rabinowitch, M. L. (1980),Enzyme Engineering: Future Directions, Wingard, L. B., Berezin, I. V., and Klyosov, A. A., eds., Plenum Press, New York, pp. 83–165.
Clementi, F. and Palade, C. E. (1969),J. Cell. Biol. 41, 33.
Squire, P. G. and Himmel, M. E. (1979),Arch. Biochem. Biophys. 196, 165.
Bertran, M. S. and Dale, B. E. (1985),Biotechnol. Bioeng. 27, 177.
Lee, Y.-H. and Fan, L. T. (1982),Biotechnol. Bioeng. 24, 2383.
Chernoglazov, V. M., Ermolova, O. V., and Klyosov, A. A. (1988),Enzyme Microb. Technol. 10, 503.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sinitsyn, A.P., Gusakov, A.V. & Vlasenko, E.Y. Effect of structural and physico-chemical features of cellulosic substrates on the efficiency of enzymatic hydrolysis. Appl Biochem Biotechnol 30, 43–59 (1991). https://doi.org/10.1007/BF02922023
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02922023