Abstract
The photosynthetic bacteriumRhodospirillum rubrum has been evaluated for its ability to produce hydrogen from carbon monoxide and water in a continuous stirred tank reactor according to the watergas shift reaction. An assessment of mass-transfer parameters and reaction kinetics was made for this sparingly soluble substrate system. Experiments were conducted in a nonsteady-state fashion with continuous liquid and gas flow, which allowed for separation of the mass-transfer and kinetic-limited regions. Based on the data obtained, mass-transfer coefficients for the system were determined, and a mathematical expression for the reaction kinetics was formulated. The results showed that the hydrogen production was inhibited by elevated levels of dissolved carbon monoxide in the liquid.
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Abbreviations
- C:
-
Concentration mol/L
- G:
-
Volumetric gas flow rate L/h
- H :
-
Henry's law constant for CO l atm/mol
- K l a :
-
overall mass-transfer coefficient h−1
- p:
-
partial pressure atm
- q:
-
Specific CO uptake rate by cells mol/g h
- r :
-
Volumetric CO mass-transfer/uptake rate mol/h L
- R :
-
Ideal gas law constant L atm/moloK
- t :
-
Time h
- T:
-
Absolute temperatureoK
- V:
-
Volume L
- X :
-
Cell concentration g/L
- *:
-
Equilibrium value
- Ar:
-
Argon, inert tracer gas
- CO:
-
carbon monoxide
- G:
-
Gas phase
- I:
-
Inlet conditions
- L:
-
Liquid phase
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Klasson, K.T., Gupta, A., Clausen, E.C. et al. Evaluation of mass-transfer and kinetic parameters forRhodospirillum rubrum in a continuous stirred tank reactor. Appl Biochem Biotechnol 39, 549–557 (1993). https://doi.org/10.1007/BF02919017
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DOI: https://doi.org/10.1007/BF02919017