Abstract
In synchronous populations ofScenedesmus quadricauda the RNA amount in the cells increases in waves: periods of a high rate of RNA synthesis alternate with periods of a low rate in the course of the cell cycle. Each wave usually leads to the doubling of the RNA amount per cell. In cells growing under normal conditions the waves of RNA synthesis seem to be linked with consecutive rounds of DNA replication. The pattern of RNA synthesis in the course of the cell cycle, however, does not change, if DNA replication is prevented by application of 5-fluoro-deoxyuridine. In darkness the rate of RNA synthesis drops to zero and thereafter the RNA amount per cell decreases. In cells which have been induced to cellular division RNA synthesis may become restored in the dark in newly formed daughter cells. The lowering of RNA amount and its new increase during the dark period become more pronounced with increasing irradiance in the previous light period as well as with its increasing length. In the period of protoplast fissions RNA synthesis is arrested even if the cells divide in the light; whether a similar inhibition occurs during mitoses is not clear.
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Bartos, J., Tetík, K.: [The syntheses of nucleic acids during the cell cycle ofScenedesmus quadricauda.] In Russ. - In: Necas, J. (ed.): Studies of the Intensive Culture of Algae II. Pp. 155–165. Trebon 1975.
Berková, E., Doucha, J., Kubín, S., Zachleder, V., Setlík, I.: Variation in photosynthetic characteristics ofScenedesmus quadricauda during the cell cycle. - In: Forti, G., Avron, M., Melandri, A. (ed.): Proc. IInd Int. Congr. Photosynthesis. Pp. 2619–2632, Stresa, Italy 1972.
Brezina, V., Hrib, J., Komárek, J.: Morphological changes in the cells ofScenedesmus quadri- cauda during ontogeny. - Arch. Hydrobiol. (Suppl. 41), Algol. Stud.6: 57–71, 1972.
Darley, W. M., Sullivan, C. W., Volcani, B. E.: Studies on the biochemistry and fine structure of silica shell formation in diatoms. - Planta130: 159–167, 1976.
Doucha, J.: Continuous cultures of algae. - In:Marvan, P., Pribil, S..Lhotský, O. (ed.): Algal Assays and Monitoring Eutrophieation. Pp. 181–191. E. Schweizerbartsche Verlag- buchhandlung (Nägele und Obermiller), Stuttgart 1979.
Edmunds, L. N., Jr.: Studies on synchronously dividing cultures ofEuglena gracilis Klebs (strain Z). II. Patterns of biosynthesis during the cell cycle. - J. Cell Comp. Physiol.66: 159 to 182, 1965.
Enöckl, F.: Änderung des tRNS- und rRNS-Gehalts vonChlorella pyrenoidosa während der Entwicklung. -Z. Pflanzenphysiol.58: 240–247, 1968.
Hopkins, H. A., Flora, J. B., Schmidt, R. R.: Periodic DNA accumulation during the cell cycle of a thermophilic strain ofChlorella pyrenoidosa. - Arch. Biochim. Biophys.153: 845 to 849, 1972.
Kanazawa, T., Kanazawa, K., Kirk, M. R., Bassham, J. A.: Difference in nitrate reduction in “light” and “dark” stages of synchronously grownChlorella pyrenoidosa and resultant metab- olic changes. - Plant Cell Physiol.11: 445–452, 1970.
Kirk, P. R., Leech, R. M.: Amino acid biosynthesis by isolated chloroplasts during photosyn- thesis. - Plant Physiol.50: 228–234, 1972.
Knutsen, G., Liest, T., Skoog, L.: Deoxyribonucleoside triphosphate and DNA synthesis in synchronized cultures ofChlamydomonas. - Exp. Cell Res.83: 442–445, 1974.
Kitbín, S.: Measurement of radiant energy. - In:Sesták, Z., Catský, J., Jarvis, P. G. (ed.): Plant Photosynthetic Production. Manual of Methods. Pp. 702–765. Dr. W. Junk, Publ., The Hague 1971.
Lien, T., Knutsen, G.: Synchronous growth ofChlamydomonas reinhardtii (Chlorophyceae): A review of optimal conditions. - J. Phycol.15: 191–200, 1979.
Losada, M.: Metalloenzymes of the nitrate-reducing system. - J. Mol. Catal.1: 245–264, 1975/76.
Lukavský, J., Tetík, K., Vendlová, J.: Extraction of nucleic acids from the algaScenedesmus quadricauda. - Arch. Hydrobiol. (Suppl. 41) Algol. Stud.9: 416–426, 1973.
Maaløe, O., Kjeldgaard, N. O.: Control of macromolecular synthesis. - W. A. Benjamin Inc., New York 1966.
Magalhaes, A. C., Neyra, C. A., Hageman, R. H.: Nitrite assimilation and amino nitrogen synthesis in isolated spinach chloroplasts. - Plant Physiol.53: 411–415, 1974.
Mann, N., Carr, N. G.: A constant ratio of transfer to ribosomal ribonucleic acid inAnacystis nidulans grown with differing mean generation times. - Biochem. Soc. Trans.1: 702–704, 1973.
Martin, T. F. J.: Paradoxical effects of protein synthesis inhibitors on uridine uptake in cul- tured cells: Possible role of uncharged tRNA in regulating metabolism. - J. Cell Physiol.103: 489–502, 1980.
Mihara, S., Hase, E.: Studies on the vegetative life cycle ofChlamydomonas reinhardi Dan- geard in synchronous culture. I. Some characteristics of the cell cycle. - Plant Cell Physiol.12: 225–236, 1971.
Mitchtson, J. M.: The Biology of the Cell Cycle. - Cambridge University Press, Cambridge 1971.
Morumura, Y.: Synchronous culture ofChlorella. I. Kinetic analysis of the life cycle ofChlorella ellipsoidea as affected by changes of temperature and light intensity. - Plant Cell Physiol.1: 49–62, 1959.
Setlík, I., Berková, E., Doucha, J., Kubín, S., Vendlová, J., Zachleder, V.: The coupling of synthetic and reproduction processes inScenedesmus quadricauda. - Arch. Hydrobiol. (Suppl. 41.) Algol. Stud.7: 172–213, 1972.
Setlík, I., Setlíková, E., Masojídek, J., Zachleder, V., Kaltna, T., Mader, P.: The effect of translation and transcription inhibitors on the development of the photosynthetic apparatus in cell cycles ofScenedesmus quadricauda. - In:Akoyunoglou, G. (ed.): Photosynthesis V. Chloroplast Development. Pp. 481–490. Balaban International Science Services, Phila- delphia 1981.
Steenbergen, C. L. M.: The role of light in the life cycle ofScenedesmus obliquus (Turp.) Kütz. - Ph. D. Thesis, Univ. Utrecht 1974.
Sulek, J.: Nuclear division inScenedesmus quadricauda (Turp.) Bréb. - Arch. Hydrobiol. (Suppl. 46) Algol. Stud.12: 224–258. 1975.
Tetík, K., Necas, J.: The cell cycles ofChlamydomonas geitleri Ettl. - Arch. Protistenk.122: 201–217, 1979.
Wanka, F.: Untersuchungen über die Wirkung des Lichts auf Zellteilung vonChlorella pyrenoi- dosa. - Arch. Mikrobiol.34: 161–188, 1959.
Wanka, F.: Die Bestimmung der Nucleinsäuren inChlorella. Kulturen. - Planta58: 594–609, 1962.
Wanka, F.: The effect of light on DNA synthesis and related processes in synchronous cultures ofChlorella. - Arch. Mikrobiol.58: 257–269, 1967.
Wanka, F.: Ultrastructural changes during normal and colchicine inhibited cell division ofChlorella. - Protoplasma66: 105–130, 1968.
Wilson, R., Chiano, K.: Temporal programming of chloroplast and cytoplasmic ribosomal RNA transcription in the synchronous roll cycle of Chlamydomonasreinhardtii. - J. Cell Biol.72: 470–481, 1977.
Zachleder, V., Doucha, J., Berkovä, E., Setlík, I.: The effect of synchronizing dark period on populations ofScenedesmus quadricauda. - Biol. Plant.17: 416–433, 1975.
Zachleder, V., Zachlederová, M., Setlík, I.: Fluorescence microscopy of acridine orange stained nuclei in chlorococcal algae. - Arch. Hydrobiol. (Suppl. 46) Algol. Stud.11: 185 to 203, 1974.
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Zachleder, V., Setlík, I. Effect of irradiance on the course of RNA synthesis in the cell cycle ofScenedesmus quadricauda . Biol Plant 24, 341–353 (1982). https://doi.org/10.1007/BF02909100
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DOI: https://doi.org/10.1007/BF02909100