Abstract
DNA from 22 different species, accessions, cultivars and lines included in theSecale genus were analyzed by the polymerase chain reaction (PCR), using as primers five pairs of oligonucleotides derived from specific sequences. A total of 42 amplified bands were considered, and some of them appeared to be potentially useful as molecular markers for some of the analyzed groups. These amplified bands were used to generate molecular phenograms inside theSecale genus.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Appels, R., Dennis, E.S., Smith, D.R., Peacoock M.J.: Two repeated sequences from heterochromatic regions in rye chromosomes.—Chromosoma84: 265–277, 1999.
Benito, C., Figueiras, A.M., Zaragoza, C., Gallego, F.G., De La Peña, A.: Rapid identification ofTriticeae genotypes from single seeds using the Polymerase Chain Reaction.—Plant mol. Biol.21: 181–183, 1993.
Dellaporta, S.L., Wood, J., Hicks, J.B.: A plant DNA minipreparation. Version II.—Plant mol. Biol. Rep.1: 19–21, 1983.
Demeke, T., Adams, R.P., Chibbar, R.: Potential taxonomic use of Random Amplified Polymorphic DNA (RAPD), a case study inBrassica L.—Theor. appl. Genet.84: 990–994, 1992.
Fritch, P., Hanson, M.A., Spore, C.D., Pack, P.E., Rieseberg, L.H.: Constancy of RAPD primer amplification strength among distantly related taxa of flowering plants.—Plant mol. Biol. Rep.11: 10–20, 1993.
Gausing, K., Barkardottir, R.: Structure and expresion of ubiquitin genes in higher plants.—Eur. J. Biochem.158: 57–62, 1986.
Hardrys, H., Balick, M., Schierwater, B.: Applications of random amplified polymorphic DNA (RAPD) in molecular ecology.—Mol. Ecol.1: 55–63, 1992.
Hart, G.E., Gale, M.D., McIntosh, R.A.: Linkage maps ofTriticum aestivum (hexaploid wheat, 2n=42, genomes A, B and D) andT. tauschii (2n=14, genome D)—In: O’Brien, S.J. (ed.): Genetic Maps. Cold Spring Harbor Laboratory, New York 1993.
Khush, G.S.: Cytogenetic and evolutionary studies inSecale II. Interrelationships of the wild species. —Evolution16: 484–496, 1962.
Khush, G.S., Stebbins, G.L.: Cytogenetic and evolutionary studies inSecale. Some new data on the ancestry ofSecale.—Amer. J. Bot.48: 723–730, 1961.
Knox, C.A., Sonthayanon, B., Chandra, G.R.: Structure and organization of two divergent alphaamylase genes from barley.—Plant mol. Biol.9: 3–17, 1987.
Kreis, M., Forde, B.G., Rahman, S., Minflin, B.J., Shewry, P.R.: Molecular evolution of the seed storage proteins of barley, rye and wheat.—J. mol. Biol.183: 499–502, 1985.
Marchionni, M., Gilbert, W.: The triosephosphate isomerase gene from maize: introns antedate the plant-animal divergence.—Cell46: 133–141, 1986.
Nei, M.: Estimation of average heterozygosity and genetic distance from number of individuals.— Genetics89: 583–590, 1978.
Nei, M., Stephens, J.C., Saitou, N.: Methods for computing the standard errors of branching points in an evolutionary tree and their application to molecular data from humans and apes.—Mol. biol. Evol.2: 66–85, 1985.
Nei, M., Tajima, F., Taneto, Y.: Accuracy of estimated phylogenetic trees from molecular data. 2. Gene frecuency data.—J. mol. Evol.19: 153–170, 1983.
Rohf, J.: NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System. Version 1.6.—Exeter Software, New York 1990.
Song, K., Osborconn, T.C., Williams, P.H.:Brassica L. taxonomy based on nuclear restriction fragment length polymorphism (RFLPs)—Theor. appl. Genet.79: 497–506, 1990.
Taneto, Y., Nei, M., Tajima, F.: Accuracy of estimated phylogenetic trees from molecular data. 1. Distantly related species.—J. mol. Evol.18: 387–404, 1982.
Vences, F.J., Vaquero, F., Pérez De La Vega, M.: Phylogenetic relationships inSecale: an isozymatic study.—Plant Syst. Evol.157: 33–47, 1987.
Zhukovsky, P.M.: A new wild growing form of rye in Anatolia.—Bull. appl. Bot.19: 49–56, 1926.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Del Pozo, J.C., Figueiras, A.M., Benito, C. et al. PCR derived molecular markers and phylogenetic relationships in theSecale genus. Biol Plant 37, 481–489 (1995). https://doi.org/10.1007/BF02908823
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02908823