Abstract
Two photosystem I (PSI) preparations were purified by non-denaturing SDS-PAGE or sucrose gradient ultracentrifugation and examined as to their chlorophyll-protein composition. In both preparations a minimum of two chlorophyll-proteins can be distinguished in addition to the 110 kDP-700 Chl a -P1 complex. One (LHCI-730) is a chlorophyll-protein (Mr 40 kD) having a high chlorophylla/b ratio, and a major 77 K fluorescence peak at 730 nm. It consists of three polypeptides with apparent molecular weights of 21, 22.5 and 24 kD. Another chlorophyll-protein (LHCI-680) with a lower molecular weight (Mr 25 kD) fluoresces at 77 K with a maximum at 680 nm. This chlorophyll-protein has a high chlorophyllb content and two constituent polypeptides of 20 and 25 kD. Absorption, 77 K fluorescence and circular dichroism spectra of the PSI related chlorophyll-proteins are presented and compared with those of photosystem II chlorophyll-proteins from maize thylakoids. We propose a model for energy transfer in the photosystem I reaction centre with the following sequence: LHCI-730→LHCI-680 →Chl a -P1. LHCI-680 acts as a connecting antenna which can also transfer energy from Chl a/b -P2. This model was used to interpret the 77 K fluorescence emission from two barley mutants.
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Abbreviations
- CD:
-
circular dichroism
- LHCI:
-
light-harvesting complex of photosystem I
- LHCII:
-
light-harvesting complex of photosystem II
- PAGE:
-
polyacrylamide gel electrophoresis
- PS:
-
photosystem
- SDS:
-
sodium dodecylsulphate
- tricine:
-
N-(tris(hydroxymethyl)methyl)glycine
- Tris:
-
tris-(hydroxymethyl)amino methane
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Bassi, R., Machold, O. & Simpson, D. Chlorophyll-proteins of two photosystem I preparations from maize. Carlsberg Res. Commun. 50, 145–162 (1985). https://doi.org/10.1007/BF02907142
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DOI: https://doi.org/10.1007/BF02907142