Abstract
Synaptonemal complex formation and distribution of recombination nodules were analyzed in trivalents of triploid Coprinus cinereus. The analysis of 14 completely reconstructed nuclei covering the period from early pachytene to mid diplotene has permitted the following conclusions: at pachytene, chromosome pairing and synaptonemal complex formation were almost exclusively in the form of trivalents. All three homologues were frequently associated at the same site, two central regions combining the three lateral components. The three homologues were never paired with three central regions. The trivalent configurations were maintained unaltered throughtout pachytene and at least the centromeres remained associated at early and mid diplotene. At mid-late pachytene, unpaired chromosome arms were frequently associated with central region material in some cases containing recombination nodules. The free face of such central regions was occasionally bound to an electron dense rod resembling a lateral component. Both central regions of the double complexes in the trivalents were able to bind recombination nodules. An analysis of the distribution of recombination nodules along the trivalents indicated that nodules were distributed independently between the two central regions of the trivalents.
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Byers, B. &L. Goetsch: Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae. Proc. Nat. Acad. Sci. USA 72, 5056–5060 (1975)
Beadle, G. W.: Crossing-over in attached-X triploids of Drosophila melanogaster. J. Genet. 29, 277–308 (1934)
Beadle, G. W.: Crossing over near the spindle attachment of the X chromosomes in attached-X triploids of Drosophila melanogaster. Genetics 20, 179–191 (1935)
Belling, J.: Chiasmas in flowering plants. Univ. Calif. Pub. Bot. 16, 311–338 (1931)
Borkhardt, B. &L. W. Olson: Meiotic prophase in diploid and tetraploid strains of Allomyces macrogynus. Protoplasma 100, 323–343 (1979)
Bridges, C. B. &E. G. Anderson: Crossing over in the X chromosomes of triploid females of Drosophila melanogaster. Genetics 10, 418–441 (1925)
Carpenter, A. T. C.: Synaptonemal complex and recombination nodules in recombination deficient mutants of Drosophila melanogaster. Chromosoma (Berl.) 75, 259–292 (1979)
Comings, D. E. &T. A. Okada: Triple chromosome pairing in triploid chickens. Nature 231, 119–121 (1971)
Darlington, C. D. &K. Mather: The origin and behaviour of chiasmata, III. Triploid Tulipa. Cytologia 4, 1–15 (1932)
Hobolth, P.: Chromosome pairing in allohexaploid wheat var. Chinese Spring. Transformation of multivalents into bivalents, a mechanism for exclusive bivalent formation. Carlsberg Res. Commun. 46, 129–173 (1981)
Holm, P. B.: Three dimensional reconstruction of chromosome pairing during the zygotene stage of meiosis in Lilium longiflorum (Thunb.). Carlsberg Res. Commun. 42, 103–151 (1977)
Holm, P. B. &S. W. Rasmussen: Human meiosis III. Electron microscopical analysis of chromosome pairing in an individual with a balanced translocation 46, XY, t(5p−; 22p+). Carlsberg Res. Commun. 43, 329–350 (1978)
Holm, P. B., S. W. Rasmussen, D. Zickler, B. C. Lu, &J. Sage: Chromosome pairing, recombination nodules and chiasma formation in the basidiomycete Coprinus cinereus. Carlsberg Res. Commun. 46, 305–346 (1981)
Lu, B. C.: Meiosis in Coprinus lagopus: A comparative study with light and electron microscopy. J. Cell Sci. 2, 529–536 (1967)
Lu, B. C.: Genetic recombination in Coprinus. I. Its precise timing as revealed by temperature treatment experiments. Can. J. Genet. Cytol. 11, 834–847 (1969)
Lu, B. C.: Meiosis in Coprinus. VIII. A time-course study of the fusion and division of the spindle pole body during meiosis. J. Cell Biol. 76, 761–766 (1978)
Mather, K.: The relation between chiasmata and crossing-over in diploid and triploid Drosophila melanogaster. J. Genet. 27, 243–259 (1933)
McClintock, B.: The association of non-homologous parts of chromosomes in the mid prophase of meiosis in Zea mays. Z. Zellforsch. u. mikro. Anat. 19, 191–237 (1933)
Moens, P. B.: Synaptonemal complexes of Lilium tigrinum (triploid) sporocytes. Can. J. Genet. Cytol. 10, 799–807 (1968)
Moens, P. B.: The fine structure of meiotic chromosome pairing in the triploid Lilium tigrinum. J. Cell Biol. 40, 273–279 (1969)
Moses, M. J.: Microspreading and the synaptonemal complex in cytogenetic studies. In: Chromosomes Today vol. 6, A. de la Chapelle and M. Sorsa, eds. Elsevier/North Holland Biomedical Press, Amsterdam, New York, Oxford, pp 71–82 (1977)
Moses, M. J. &P. A. Poorman: Synaptonemal complex analysis of mouse chromosomal rearrangements. II. Synaptic adjustment in a tandem duplication. Chromosoma (Berl.) 81, 519–535 (1981)
Newton, W. C. F. &C. D. Darlington: Meiosis in polyploids. I. Triploid and pentaploid tulips. J. Genet. 21, 1–15 (1929)
Olmo, H. P.: Prophase association in triploid Nicotiana tabacum. Cytologia 5, 417–431 (1934)
Rasmussen, S. W.: Chromosome pairing in triploid females of Bombyx mori analyzed by three dimensional reconstructions of synaptonemal complexes. Carlsberg Res. Commun. 42, 163–197 (1977)
Rasmussen, S. W. &P. B. Holm: Human meiosis II. Chromosome pairing and recombination nodules in human spermatocytes. Carlsberg Res. Commun. 43, 275–327 (1978)
Rasmussen, S. W. &P. B. Holm: Chromosome pairing in autotetraploid Bombyx females. Mechanism for exclusive bivalent formation. Carlsberg Res. Commun. 44, 101–125 (1979)
Rasmussen, S. W. &P. B. Holm: Mechanics of meiosis. Hereditas 93, 187–216 (1980)
Redfield, H.: Crossing over in the third chromosomes of triploids of Drosophila melanogaster. Genetics 15, 205–252 (1930)
Skovsted, A.: Cytological studies in cotton. I. The mitosis and meiosis in diploid and triploid asiatic cotton. Ann. Bot. 47, 227–251 (1933)
Solari, A. J. &M. J. Moses: Synaptonemal complex in a tetraploid mouse spermatocyte. Exp. Cell Res. 108, 464–467 (1977)
Stone, L. H. A. &K. Mather: The origin and behaviour of chiasmata, IV. Diploid and triploid Hyacinthus. Cytologia 4, 16–25 (1932)
Wettstein, D. von: The assembly of the synaptinemal complex. Phil. Trans. R. Soc. Lond. B. 277, 235–243 (1977)
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Rasmussen, S.W., Holm, P.B., Lu, B.C. et al. Synaptonemal complex formation and distribution of recombination nodules in pachytene trivalents of triploid Coprinus cinereus. Carlsberg Res. Commun. 46, 347 (1981). https://doi.org/10.1007/BF02906520
DOI: https://doi.org/10.1007/BF02906520