Abstract
Recombinant “all-fish” growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant “fast-growing” effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the F1 generation were genetically analyzed, which gave solid evidence for the hypothesis that 2–3 chromosomes are integrated with transgene. In addition, the distinct biological effects for multisite-integrated transgenes were further discussed. The present study opens a door for the breeding of “fast-growing” transgenic fish.
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Zhu, Z., Li, G., He, L. et al., Novel gene transfer into the fertilized eggs for goldfish (Carassium auratus L. 1785), Z. Angew. Ichthyol., 1985, 1: 31.
Zhu, Z., Xu, K., Li, G. et al., Biological effects of human growth hormone gene microinjected into the fertilized genes of loach Misgurnus anguillicaudatus (Cantor), Kexue Tongbao, 1986, 31(14): 988.
Dunham, R. A., Eash, J., Askins, J. et al., Transfer of the metallothioneinhuman growth hormone gene into channel catfish, Trans. Amer. Fish. Soc., 1987, 116: 87.
Penman, D. J., Beeching, A. J., Penn, S. et al., Factors affecting survival and integration following microinjection of novel DNA into rainbow trout eggs, Aquaculture, 1990, 85: 35.
Fletcher, G. L., Shears, M. A., King, M. J. et al., Evidence for antifreeze protein gene transfer in Atlantic salmon (Salmo salar), Can. J. Fish. Aqaut. Sci., 1988, 45: 352.
Shears, M. A., Fletcher, G. L., Hew, C. L. et al., Transfer, expression and stable inheritance of antifreeze protein genes in Atlantic salmon (Salmo salar), Mol. Mar. Biol. Biotechnol., 1991, 1(1): 58.
Zhu, Z., Xu, K., Xie, Y. et al., A model of transgenic fish, Science in China, Ser. B (in Chinese), 1989(2): 147.
Zhu, Z., Growth hormone gene and the transgenic fish, in Agricultural Biotechnology (eds. You, C. B., Chen, Z. L.), Beijing: China Science and Technology Press, 1992, 106.
Westhusin, M., From mighty mice to mighty cows, Nature Genetics, 1997, 17: 4.
Wang, Z., Zhu, Z., Chromosomalin situ hybridization of Cyprinus carpio genomic DNA repetitive sequence CR1, Chinese Science Bulletin, 1999, 44(24): 2242.
Zeng, Z., Zhu, Z., Transgenes in F4 pMThGH-transgenic red common carp (Cyprinus carpio L.) are highly polymorphic, Chinese Science Bulletin, 2001, 46(2): 143.
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Wang, Y., Hu, W., Wu, G. et al. Genetic analysis of “all-fish” growth hormone gene trans ferred carp (Cyprinus carpio L.) and its F1 generation. Chin.Sci.Bull. 46, a1–a4 (2001). https://doi.org/10.1007/BF02900596
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DOI: https://doi.org/10.1007/BF02900596