Abstract
A cross-sectional survey was undertaken to study the aging effect on platelet cytoplasmic membrane outer leaflet fluidity and Na+/H+ antiporter activity, and to investigate the relative influence of membrane order and age on the Na+/H+ exchanger. The study population consisted of 19 healthy subjects (age range 22–83 years, mean age 48.5 ± 16.5 years; 12 females and 7 males). Washed platelets were used as neuronal models and as eventually contributing to brain pathology by aberrant secretion of their granule content. A significant positive correlation (r = 0.48,p < 0.05) was found between age and the membrane structural order, according to a second-degree equation. Sewall-Wright path coefficient analysis revealed that the age influence on Na+/H+ exchanger activity was unimportant (0.18), but those of cytoplasmic membrane outer leaflet fluidity and mean platelet volume were strong and positive (0.51 and 0.54, respectively). Women presented a marked standard deviation in the countertransporter results. In conclusion, decreased membrane outer leaflet fluidity and unchanged Na+/H+ antiporter activity may contribute to the process of normal brain aging.
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Davies TA, Fine RE, Johnson RJ, Levesque CA, Rathbun WH, Seetoo KF, Smith SJ, Strohmeier G, Volicer L, Delva L, Simons ER (1993) Non-age related differences in thrombin responses by platelets from male patients with advanced Alzheimer’s disease. Biochem Biophys Res Commun 194: 537–543
Hasan M, Jackson SK, Sinclair AJ (1995) Aspirin-induced conformational changes in platelet membrane in subjects with stroke. Gerontology 41: 212–219
Igbavboa U, Avdulov NA, Schroeder F, Wood WG (1996) Increasing age alters transbilayer fluidity and cholesterol asymmetry in synaptic plasma membranes of mice. J Neurochem 66: 1717–1725
Kubina M, Lanza F, Cazenave JP, Laustriat G, Kuhry JG (1987) Parallel investigation of exocytosis kinetics and membrane fluidity changes in human platelets with the fluorescent probe, trimethylammonio-diphenylhexatriene. Biochim Biophys Acta 901:138–146
Marinho C, Januário C, Matias F, Cunha L, Oliveira C (1990) Ageing does not affect human platelet Na+/H+ antiporter. Med Sci Res 18: 567–568
Rosskopf D, Siffert G, Osswald U, Witte K, Dusing R, Akkerman JWN, Siffert W (1992) Platelet Na+-H+ exchanger activity in normotensive and hypertensive subjects: effect of enalapril upon antiport activity. J Hypert 10: 839–847
Sarmento AB, Januário C, Garrucho MH, Cunha L, Almeida L, Oliveira CR (1991) Biophysical properties of cell membranes in aging and Alzheimer’s disease. IV Portuguese-Spanish Biochemistry Congress, Póvoa de Varzim, Abstract 8 P154-Tu
Sugidachi A, Asai F, Oshima T, Koike H (1993) Age-associated changes in intracellular Ca2+ mobilization and basal pH level in rat platelets. Platelets 4: 91–95
Zubenko GS (1992) Biological correlates of clinical heterogeneity in primary dementia. Neuropsychopharmacology 6: 77–93
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Marinho, C.F.M., Oliveira, C.R., Pinto-de-Barros, J. et al. Correlation between human platelet cytoplasmic membrane outer leaflet fluidity, Na+/H+ exchanger activity and aging. Eur Arch Psychiatry Clin Nuerosci 247, 275–277 (1997). https://doi.org/10.1007/BF02900306
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DOI: https://doi.org/10.1007/BF02900306