Summary
The proliferation of villous trophoblast in the human placenta was estimated throughout normal gestation and in term placentae from preeclamptic and smoking mothers by two different methods. These were: 1) labeling of DNA producing cells by bromodeoxyuridine (BrdU) followed by immunohistochemistry using a monoclonal anti-BrdU antibody, and 2) immunohistochemical identification of all proliferating cells by the monoclonal antibody Ki67. Both methods revealed comparable results. In uncomplicated pregnancies there was a remarkable decrease in the labeling indices from early gestation to term. This was the result of a diminution of the number of Langhans’ cells, although the cell division rate within the Langhans’ cell layer remained nearly constant throughout gestation. A prolongation of the cell cycle in the cytotrophoblast cells at term was indicated by an increase in the Ki67/BrdU ratio. Compared with normal term placentae, there was an increase in the trophoblast proliferation rate in preeclampsia, but not in placentae from smoking mothers. Moreover, the number of Langhans’ cells was diminished in placentae from smokers. The results indicate that there are different pathogenetic mechanisms of placental impairment in preeclampsia and in maternal smoking. In preeclampsia an injury to the syncytiotrophoblast seems to lead to a repair hyperplasia of the cytotrophoblast, whereas in maternal smoking, there seems to be a direct toxic effect on the cytotrophoblastic cells.
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Aherne WA, Dunnhill MS (1987) Point counting and the estimation of volume fraction. In: Aherne WA, Dunnill MS (eds) Morphometry. E Arnold, London, pp 33–45
Arnholdt H, Meisel F, Bäther K, Löhrs U (1988) Trophoblastproliferation and-differenzierung in normalen und pathologischen Plazenten. Verh Dt Ges Pathol 72:497
Arnholdt H, Diebold J, Löhrs U (1990) EGF-Bindung und transsyncytialer Transport des Ligand-Rezeptor-Komplexes an Chorionzotten der menschlichen Plazenta. Verh Dt Ges Pathol 74:504
Brosens I, Renaer M (1972) On the pathogenesis of placental infarcts in pre-eclampsia. J Obstet Gynaecol Br Cwlth 79:794–799
Busacca M, Balconi G, Pietra A, Vergara-Dauden M, Gaetano G de, Dejana E (1984) Maternal smoking and prostacyclin production by cultured endothelial cells from umbilical arteries. Am J Obstet Gynecol 148:1127–1130
Diebold J, Arnholdt H, Lai MD, Löhrs U (1991) C-myc expression in early human placenta a critical evaluation of its localization. Virchows Arch [B] 61 (press)
Dixon HG, Robertson WB (1961) Vascular changes in the placental bed. Pathol Microbiol 24:622–630
Fox H (1964) The villous cytotrophoblast as an index of placental ischaemia. J Obstet Gynaecol Br Cwlth 71:885–893
Fox H (1983) The pathology of extravillous trophoblast. Arch Anat Cytol Pathol 31:73–74
Gerbie AB, Hathaway HH, Brewer JI (1968) Autoradiographic analysis of normal trophoblastic proliferation. Am J Obstet Gynecol 100:640–648
Gerdes J, Lemke H, Baisch H, Wacker H-H, Schwab U, Stein H (1984) Cell cylce analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol 133:1710–1715
Gille J (1986) Schwangerschaftsinduzierte Hypertonie. In: Künzel W, Wulf K-H (eds) Klinik der Frauenheilkunde und Geburts-hilfe. Vol 5, Die gestörte Schwangerschaft., ed. 2. Urban und Schwarzenberg, München Wien Baltimore, pp 32–33
Hustin J, Foidart JM, Lambotte R (1984) Cellular proliferation in villi of normal and pathological pregnancies. Gynecol Obstet Invest 17:1–9
Jones CJP, Fox H (1980) An ultrastructural and ultrahistochemical study of the human placenta in maternal pre-eclampsia. Placenta 1:61–76
Jones CJP, Fox H (1981) An ultrastructural and ultrahistochemical study of the human placenta in maternal essential hypertension. Placenta 2:193–204
Kaltenbach FJ, Fettig O, Krieger ML (1974) Autoradiographische Untersuchungen über das Proliferationsverhalten der menschlichen Placenta unter normalen und pathologischen Bedingungen. Arch Gynäkol 216:369–386
Karbowski B, Bauch H-J, Schneider HPG (1989) Funktionelle Differenzierung des vaskulären Endothels bei Hochrisikoschwan-gerschaften. Z Geburtsh Perinat 193:8–12
Kaufmann P (1972) Untersuchungen über die Langhanszellen in der menschlichen Plazenta. Z Zellforsch 128:283–302
Kaufmann P (1981) Entwicklung der Plazenta. In: Becker V, Schiebler ThH, Kubli F (eds) Die Plazenta des Menschen. G. Thieme, Stuttgart New York, pp 13–50
Kaufmann P, Gentzen DM, Davidoff M (1977) Die Ultrastruktur von Langhanszellen in pathologischen menschlichen Placenten. Arch Gynäkol 222:319–332
Krauer F (1970 a) Die Veränderung des Tritium-Thymidin-Index in der wachsenden Plazenta. In: Raugna D da (ed) Aktuelle Probleme in Gynäkologie und Geburtshilfe. Schwabe & Co, Basel Stuttgart, pp 114–122
Krauer F (1970b) The 3H-Thymidin-Index normaler und pathologischer Plazenten verschiedenen Alters. In: Rippmann ET (ed) Spätgestose. Schwabe & Co, Basel Stuttgart, pp 275–279
Laskey RA, Fairman MP, Blow JJ (1989) S phase of the cell cycle. Science 246:609–614
Naeye RL (1978) Effects of maternal cigarette smoking on the fetus and placenta. Br J Obstet Gynaecol 85:732–737
Pardee AB (1989) G1 events and regulation of cell proliferation. Science 246:603–608
Pfeiffer-Ohlsson S, Goustin AS, Rydnert J, Wahlström T, Bjersing L, Stehelin D, Ohlsson R (1984) Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: implications for embryonic cell proliferation. Cell 38:585–596
Philipp K, Pateisky N, Endler M (1984) Effects of smoking on uteroplacental blood flow. Gynaecol Obstet Invest 17:179–182
Pirani BBK (1978) Smoking during pregnancy. Obstet Gynecol Survey 33:1–13
Robertson WB, Brosens I, Dixon G (1975) Uteroplacental vascular pathology. Eur J Obstet Gynecol Reprod Biol 5:47–65
Sachs L (1988) In: Sachs L (ed) Statistische Methoden: Planung und Auswertung, 6th ed. Springer, Berlin Heidelberg New York Tokyo, p 39
Soma H, Yoshida K, Mukaida T, Tabuchi Y (1982) Morphologic changes in the hypertensive placenta. Contr Gynecol Obstet 9:58–75
Teasdale F (1985) Histomorphometry of the human placenta in maternal preeclampsia. Am J Obstet Gynecol 152:25–31
Teasdale F, Ghislaine J-J (1989) Morphological changes in the placentas of smoking mothers: a histomorphometric study. Biol Neonate 55:251–259
Toussaint M-N (1989) Rauchen und Gestose: Vergleichsergebnisse klinischer und histomorphometrischer Untersuchungen. Geburtsh Frauenheilk 49:53–57
Weinberg PC, Cameron IL, Parmley T, Jeter JR, Pauerstein CJ (1970) Gestational age and placental cellular replication. Obstet Gynecol 36:692–696
Wigglesworth JS (1962) The Langhans layer in late pregnancy: a histological study of normal and abnormal cases. J Obstet Gynaecol Br Cwlth 69:355–365
Winick M, Coscia A, Noble A (1967) Cellular growth in human placenta. Pediatrics 39:248–251
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Arnholdt, H., Meisel, F., Fandrey, K. et al. Proliferation of villous trophoblast of the human placenta in normal and abnormal pregnancies. Virchows Archiv B Cell Pathol 60, 365–372 (1991). https://doi.org/10.1007/BF02899568
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DOI: https://doi.org/10.1007/BF02899568