Abstract
The cell volume fraction ofSaccharomyces cerevisiae accessible tod-xylose and other nonmetabolized sugars was found to correspond to 100% of cell water in resting yeast over a range of osmolarities of the medium. This sugar space was decreásed to 60% by nitrogen deficiency and to 82% by phosphorus deficiency. The space of N-deficient cells was significantly raised by pre-incubation with ammonium chloride, amino-acids and nucleotides from the yeast extract and by adenosine triphosphate while that of P-deficient cells was not significantly altered by analogous treatment. The xylose space was found to be markedly lower in growing than in resting cells. Urea (0.3–6%) had a depressing effect on the sugar space size while a variety of metabolic inhibitors were without influence. TheK m andV values ofd-xylose uptake bySaccharomyces cerevisiae R XII at 30° were found to be 180–192 mM and 680–730 mg. xylose/ml. cell volume/hr., respectively. These values were not affected by N-deficiency and by enrichment with adenosine triphosphate. The xylose space size was shown to be practically identical with that of urea space and its decrease was related to the increase of “bound” water within the cell under various conditions studied. The changes of space are tentatively attributed to changes of hydration and dehydration of cellular components.
Abstract
Участок объема клетки дрожжей Saccharomyces cerevisiae, досуіный для D-ксилозы и других не подвергающихся обмену caxapob, отвечает 100% клеточной воды у дрожжей в сосотянии покоя в пределах широкого спектра осмолярности среды. При недостатке азота зтот участок уменыпался до 60%, а при недостатке фосфора—до 80%. У N-дефицитных клеток зто пространство можно было существенно увеличить с помощью предварительной инкубации с хлористым аммонием, аминокислотами и нуклеотидами из дрожжевого зкстракта, а также с аденозинтрифосфатом, тогда как на P-дефицитные клетки подобные вмешательства не оказывали действия. У растущих клеток ксилозодступное пространство заметно меньше, чем у клеток у покое. Обработка мочевиной (0,3–6,0%) уменьшала размеры ксилозодоступного пространства, тогда как различные ингибиторы метаболизма не оказывали влияния. Величины Km и V для включения D-ксилозы клетками Saccharomyces cerevisiae R XII составляют 180–192 мМ или же 680–730 мг ксилозы/мл клеточного обьема/час. На эти величины не оказывали действия ни недостаток азота, ни обогащение аденозинтрифосфатом. Было установлено, что размеры ксилозодоступного пространства практически сходны с размерами пространства, доступного для проникновения мочевины, и что их уменьшение находится в связи с увеличением содержания воды, связанной в клетках при различных условиях опыта.—Изменения этого пространства предположительно относятся за счет изменений в гидратации и дегидратации белков.
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Kotyk, A., Kleinzeller, A. Transport of D-xylose and sugar space in baker's yeast. Folia Microbiol 8, 156–164 (1963). https://doi.org/10.1007/BF02894974
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DOI: https://doi.org/10.1007/BF02894974