Summary
To evaluate the effect of estrogen on its ability to restore the bone mass and bone quality in ovariectomized rats by examining the changes of bone morphology and histomorphometry, 3-month-old rats were divided randomly into 4 groups: normal control, ovariectomized (OVX), shamoperated (Sham-O) and OVX plus estrogen (OVX+E2). Treatment initiated from the day 8 weeks after operation and continued for 12 weeks. Bone morphology and histomorphometry were examined afterwards. Results showed that comparing to control group, the trabecular bone in OVX appeared thinner and reduced in the amount. The connectivity between trabecula was decreased and the structure disordered. The free-end of trabecula was increased. The cavity of bone marrow enlarged. After treatment with estrogen, above changes improved remarkably by different degree, although did not reach the normal face. The bone histomorphometry results damonstrated that estrogen treatment increased bone mass and the amount of trabecula by 129 % and 132 % respectively (P < 0. 05). The activity of bone resorption decreased significantly and the rate of bone formation increased to 203%. These results suggest that treatment of ovariectomized rats with estrogen can not only increase bone mass, also improve the bone structure and enhance the property of bone mechanics.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Dempster D W, Lindsay R. Pathogenesis of osteoporosis. Lancet, 1993, 341: 79
Dempster D W, Shane E, Horbert Wet al. A simple method for correlative light and scanning electron microscopy of human iliac crest bone biopsies: qualitative observations in normal and osteoporotic subjects. J Bone Miner Res, 1986, 1: 15
Parfitt A M. Trabecular bone architecture in the pathogenesis and prevention of fracture. Am J Med, 1987, 82: 68
Rocker R R. Techniques and Interpretation. In: Rocker R R (ed). Bone histomorphometry. Florida: CRC Press, 1983. 14–24
Frost H M. On rho, a marrow mediator and estrogen: their roles in bone strength and “mass” in humen females, osteopenias and osteoporosis. J Bone Miner Metab, 1998, 16: 113
Miyaura C, Kusano K, Masuzawa Tet al. Endogenous bone-resorbing factors in estrogen deficiency: cooperative effects of IL-1 and IL-6. J Bone Miner Res, 1995, 10: 1365
Kitazawa R, Kimble R B, Vannice J Let al. Interleukin1 receptor antagonist and tumor necrosis factor binding protein decrease osteoclast formation and bone resorption in ovariectomized mice. J Clin Invest, 1994, 94: 2397
Stavros C M, Robert L J. Bone marrow, cytokines, and bone remodeling. The New Eng J of Med, 1995, 332 (5): 305
Author information
Authors and Affiliations
Additional information
This project was supported by a grant from the National Natural Science Foundation of China (No. 39770930).
Rights and permissions
About this article
Cite this article
Lulu, C., Tianshu, Z., Wenfang, X. et al. The effect of estrogen on the Restoration of bone mass and bone quality in ovariectomized rats. Current Medical Science 20, 283–286 (2000). https://doi.org/10.1007/BF02888180
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02888180