Abstract
A pot experiment has been carried out under controlled conditions to study the possibility of applying the technique ofin vivo staining for acid phosphatase activity on the roots of mycorrhizal plants and arbuscular mycorrhizal hyphae. The pots had 5 compartments. The central root compartment was separated from the two adjacent hyphal compartments using nylon nets of 30 μm mesh, and the two hyphal compartments were separated from the two outermost compartments with 0.45 μm membranes. Red clover was grown in the root compartment and was either inoculated with the arbuscular mycorrhizal fungus (AMF)Glomus mosseae or unlnoculated. Sodium phytate was applied to all compartments. The results show that AMF can increase acid phosphatase activity of clover roots. The plant roots acquired deep red “mycorrhizal prints”. The external hyphae also had obvious “hyphal prints” on the test papers, indicating the ability of mycorrhizal hyphae to release acid phosphatase.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Dinkelaker, B., Marschner, H.,In vivo demonstration of acid phosphatase activity in the rhizosphere of soil-grown plants, Plant and Soil, 1992, 144: 199.
Warren, R. J., Moss, D. W., An automated continuous monitoring procedure for the determination of acid phosphatase activity in serum, Clin. Chim. Acta, 1977, 77: 179.
Gundlach, G., Mühlhausen, B., Untersuchungen zur Kupplung des 1-Naphthols mit Fast-red-TR. Untersuchungen zur Optimierung einer kontinuierlichen Bestimmung der sauren phosphatase, 1. Mitteilung, J. Clin. Chem. Clin. Biochem., 1980, 18: 603.
Shaykh, M. M., Roberts, L. W., A histochemical study of phosphatases in root apical meristems, Ann. Bot., 1974, 38: 165.
Felipe, M. R., Pozuelo, J. M., Cintas, A. M., Acid phosphatase localization at the surface of young corn roots, Agrochim., 1979, 23: 143.
Tarafdar, J. C., Claassen, N., Organic phosphorus compounds as a phosphorus source for higher plants through the activity of phosphatases produced by plant roots and microorganisms, Biol. Fertil Soils, 1988, 5: 308.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Song, Y., Li, X., Feng, G. et al. Rapid assessment of acid phosphatase activity in the mycorrhizosphere and in arbuscular mycorrhizal fungal hyphae. Chin.Sci.Bull. 45, 1187–1191 (2000). https://doi.org/10.1007/BF02886076
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02886076