Abstract
The ability of the yeastCandida lipolytica 4-1 to oxidize and utilize various pure aliphatic hydrocarbons occurring in gas oil was studied. It was found that the given strain ofCandida lipolytica oxidized n-alkanes without adaptation, starting with heptane, and utilized them for growth, starting with nonane. Isoalkanes with a single methyl group in the side chain were also oxidized and utilized for growth, but less than the corresponding n-alkanes. The site of the methyl group in the isoalkane chain influences its conversion to biomass. Branched chains at both ends of the isoalkane molecule prevent its utilization for growth ofCandida lipolytica. 1-olefines are also oxidized and utilized for growth, though less than the corresponding n-paraffins. Alkylaromatic hydrocarbons are oxidized from amylbenzene up to decylbenzene, which is utilized only slightly for growth of the yeast.
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Asinger, F.:Verfahren zur quantitativen oxydativen Aufspaltung von höhermolekularer Olefine. Ber. 75: 658, 1942.
Bláha, K.:Preparative reactions in organic chemistry VI. (In Czech) Publ. House Czech. Acad. Sci., Prague 1961.
Brooks, B. T., Kurtz, S. S., Boord, C. E., Schmerling, E.:The Chemistry of petroleum hydrocarbons. (Chinese reprint.) Vol. 1, p. 506, 1954.
Buchta, E., Weidinger, H.:Synthese mit 1,4-Butylendimagnesium-bromid. Ann. 580: 109, 1953.
Canin, D. L., Forziati, A. F., Rossini, F. D.:Physical properties of n-hexadecane, n-decylcyclopentane, n-decylcyclohexane, 1-hexadecane and n-decylbenzene. J. phys. Chem. 58: 440, 1954.
Champagnat, A., Vernet, C., Laine, B., Filosa, J.:Biosynthesis of proteinvitamin concentrate of petroleum. Nature 197: 13, 1963a.
Champagnat, A., Vernet, C., Laine, B., Filosa, J.:Déparaffinage microbiologique avec production de concentrates protéines-vitamines. Proc. VI. World Petroleum Congress, Frankfurt am Main, Sec. IV, paper 4—PD 10, 1963b.
Diuguid, L. I.:The use of amalgamated aluminium as a catalyst in the Frieden and Crafts reaction. J. Am. chem. Soc. 63: 3527, 1941.
Dostálek, M., Munk, V., Volfová, O., Pecka, K.:Cultivation of the yeast Candida lipolytica on hydrocarbons. I. Degradation of n-alkanes in batch fermentation of gas oil. Biotechnol. & Bioeng. 10: 33, 1968a.
Dostálek, M., Munk, V., Volfová, O., Fencl, Z., Pecka, K.:Cultivation of the yeast Candida lipolytica on hydrocarbons. II. One-stage continuous cultivation on gas oil. Biotechnol. & Bioeng. 10: 865, 1968b.
Edgar, G., Calingaert, G., Marer, R. E.:The preparation and properties of the isomeric heptanes. I. Preparation. J. Am. chem. Soc. 51: 1483, 1929.
Fahim, H. A., Mustafa, A.:A comparative study of the Wurtz-Fittig and the Clemmensen method for the preparation of aromatic hydrocarbons. J. chem. Soc. (London) 519, 1949.
Faillebin, M.:Sur la préparation de l'hexan normal. Bull. Soc. Chim. Fr. 35: 160, 1924.
Grignard, V.:Les récentes acquisitions des méthodes organomagnésiennes. Bull. Soc. Chim. Fr. 39: 1285, 1926.
Kazansky, B. A., Liebman, A. L., Plate, A. F., Rosengart, M. I., Tarasova, G. A.:Synthesis and physical characteristics of some 1-alkanes. (In Russian) Zhur. Obshch. Chim. 17: 1503, 1947.
Krafft, F.:Zur Darstellung höherer Olefine, insbesondere über Dodecylen C 12 H 24,Tetradecylen C 14 H 28,Ceten oder Hexadecylen C 16 H 32 und Octadecylen C 18H36. Ber. 16: 3018, 1883.
Landa, S., Kejvan, A.:Synthèse du 2,11-diméthyldodécane et du 2,19-diméthyleicosane. Coll. Czech. chem. Commun. 3: 367, 1931.
Landa, S., Mostecký, J.:Preparation of pure hydrocarbons by hydrogenation on tungsten disulphide. I. Hydrogenation of alcohols, ketones, esters and acid anhydrides. Coll. Czech. chem. Commun. 20: 430, 1955.
Landa, S., Weisser, O.:Preparation of hydrocarbons by hydrogenation on molybdenum disulphide. I. Hydrogenation of alcohols, ketones, acids and esters. (In Czech) Chem. Listy 50: 569, 1956.
Landa, S., Weisser, O.:Preparation of hydrocarbons by hydrogenation on molybdenum disulphide. I. Hydrogenation of alcohols, ketones, acids and esters. (In Russian) Coll. Czech. chem. Commun. 22: 93, 1957a.
Landa, S., Weisser, O., Mostecký, J.:Über die Eigenschaften von Sulfid-Katalysatoren. IV. Zum Mechanismus der Hydrierung sauerstoffhaltiger Verbindungen. Coll. Czech. Chem. Commun. 22: 1006, 1957b.
Landa, S., Weisser, O., Mostecký, J.:Über die Eigenschaften sulfidischer Katalysatoren. VI. Die dehydratierende und dehydrierende Eigenschaften von Molybdän-und Wolframsulfid. Coll. Czech. chem. Commun. 24: 1036, 1959.
Lavina, R. Y., Shusherina, N. P.:Methods of synthesis of paraffin hydrocarbons (alkanes). (In Russian) Usp. chim. 24: 181, 1955.
Lukeš, R., Bláha, K.:Preparation of Grignard reagents derived from ω ω-dibromo-n-olkanes. (In Czech) Chem. Listy 46: 683, 1952.
Ma, T. S., Zuazaga, G.:Microkjeldahl determination of nitrogen. A new indicator and an improved rapid method. Ind. Eng. Chem. Annual 14: 280, 1942.
Markham, N.:A stream distillation apparatus suitable for micro-kjeldahl analysis. Biochem. J. 36: 790, 1942.
Obolentsev, R. D.:Physical constants of hydrocarbons of liquid fuels and oils. (In Russian) Gostoptekhizdat, Moscow 1953.
Petrov, A. A., Serbienko, S. R., Nechitailov, N. A., Tsedilina, A. L.:Synthesis and characteristic of monomethyl-substituted alkanes of the composition C 12 −C 16. (In Russian) Izv. A. N. SSSR, Otd. Chim. Nauk, 1091, 1959.
Suida, H., Drohowzal, F.:Ceten=Hexadecen=(1). Ber. 75: 991, 1942.
Wooster, C. B.:Organo-alkali compounds. Chem. Rev. 11: 1, 1932.
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Dedicated to Academician Ivan Málek on the occasion of his 60th birthday
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Munk, V., Volfová, O., Dostálek, M. et al. Cultivation of the yeastCandida lipolytica on hydrocarbon. Folia Microbiol 14, 334–344 (1969). https://doi.org/10.1007/BF02872701
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DOI: https://doi.org/10.1007/BF02872701