Abstract
The nuclear rDNA regions of the two internal transcribed spacers (ITS1 and ITS2) and 5.8S rRNA gene from 52 field isolates ofSpongospora subterranea f.sp.subterranea obtained from the British Isles and North America were polymerase chain reaction-amplified, sequenced, and assessed for genetic variation. Two genetically distinct groups (I and II) were identified based on the ITS sequence diversity among the isolates, representing 34.6% and 65.4% of the isolates, respectively. British Isles isolates occurred in groups I and II, whereas North American isolates belonged only to group II. The British Isles groups ofS. subterranea were associated with particular potato cultivars. The full-length small-subunit rRNA gene ofS. subterranea was sequenced and analyzed by both neighbor-joining and parsimony methods to clarify the taxonomic position of this pathogen. The results of phylogenetic analysis showed thatS. subterranea grouped together with other species of plasmodiphorids, and this group clustered with the phylum Cercozoa, an assemblage of filose and reticulose amoebae and phylogenetically related zooflagellates. The recognition of the existence of different genetic groups withinS. subterranea will be important for the design of plant-breeding programs and in testing for plant resistance.
Resumen
Las regiones rADN nuclear de los dos espaciadores transcrites internos (ITS1 e ITS2) y el gen 5.8S rARN de 52 aislamientos de campo deSpongospora subterranea f.sp.subterranea obtenidos de las Islas Británicas y de Norteamérica fueron amplificadas por reacción en cadena de la polimerasa, secuenciadas y evaluadas para variación genética. Se identificaron dos grupos genéticamente diferentes (I y II) en base de la diversidad de frecuencia ITS entre los aislamientos, lo que representa el 34.6% y el 65.4% de los aislamientos respectivamente. En los aislamientos de las Islas Británicas se encontró los grupos I y II, mientras que los aislamientos de Norteamérica pertenecían sólo al grupo II. Los grupos deS. subterranea de las Islas Británicas estuvieron asociados con cultivares especiales de papa. El tamaño complete de la sub-unidad del gen rARN deS. subterranea fue secuenciado y analizado por los métodos “neighborjoining y parsimony”, con el objeto de aclarar la posición taxonómica de este patógeno. El resultado del análisis filogenético demostró queS. subterranea junto con otras especie de plasmodiophoridos está relacionado con el phylum Cercozoa que incluye un conjunto de amebas filiformes y zooflagelados filogenéticamente relacionados. El reconocimiento de la existencia de diferentes grupos genéticos dentro deS. subterranea será importante para el diseño de programas de mejoramiento y pruebas de resistencia de la planta.
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Qu, X., Christ, B.J. Genetic variation and phylogeny ofSpongospora subterranea f.sp.subterranea based on ribosomal DNA sequence analysis. Am. J. Pot Res 81, 385–394 (2004). https://doi.org/10.1007/BF02870199
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DOI: https://doi.org/10.1007/BF02870199