Abstract
A kinetic test evolved for distinguishing between mobile carrier transport in which one or two substrate molecules are transported at a time was applied to sugar transport inSaccharomyces cerevisiae and it was found that the mechanism here involves attachment of one sugar molecule to one molecule of carrier.
Incidental to the test, the dissociation constants of some sugar-carrier complexes were determined.
The diversity of sugar transport mechanisms in different cells is discussed.
Abstract
Кинетический тест, разработанный для распознавания транспорта подвижным переносчиком, когда одна или две молекулы субстрата перемещаются одновременно, был использован при изучении транспорта сахара у дрожжей Saccharomyces cerevisiae. Было установлено, что этот механизм включает связывание 1 молекулы сахара с 1 молекулой переносчика. При тестировании были определены константы диссоциации некоторых комплексов сахар-переносчик. —Обсуждается различие механизмов транспорта сахара в различных клетках.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Burger, M., Hejmová, L., Kleinzeller, A.:Transport of some mono-and di-saccharides into yeast cells. Biochem. J. 71: 233, 1959.
Burger, M., Hejmová, L.:The effect of serum of rabbit immunized with crystalline hexokinase on sugar uptake by the yeast Saccharomyces cerecisiae R XII. Fol. microbiol. 6: 210, 1961.
Cirillo, V. P.:Entry of sorbose into the yeast cell. Bact. Proc., p. 108, 1959.
Cirillo, V. P.:The mechanism of sugar transport into the yeast cell. Trans. N. Y. Acad. Sci. ser. II, 23(8): 725, 1961.
Crane, R. K.:Intestinal absorption of sugars. Physiol. Rev. 40: 789, 1960.
Kepes, A.:Etudes cinétiques sur la galactoside-perméase d'Escherichia coli. Biochim. biophys. Acta 40: 70, 1960.
Kleinzeller, A., Kotyk, A.:Cations and transport of galactose in kidney-cortex slices. Biochem. biophys. Acta 54: 367, 1961.
Kotyk, A., Kleinzeller, A.:Transport of D-xylose and sugar space in baker's yeast. Fol. microbiol. 8: 156, 1963.
Kotyk, A., Wilbrandt, W.:Der Trägerkomplex des Zuckertransports durch die Erythrocytenmembran. Helv. physiol. Acta 21: C33, 1963.
Kotyk, A., Höfer, M.:Up-hill transport of sugars in the yeast Rhodotorulla gracilis. I. Kinetics. Biochem. biophys. Acta, in press.
Krane, S. M., Crane, R. K.:The accumulation of D-galactose against a concentration gradient by slices of rabbit kidney cortex. J. biol. Chem. 234: 211, 1958.
Okada, H., Halvorson, H. O.:Gene-controlled facilitated diffusion and active transport of a-thioethylglucopyranoside in Saccharomyces cerevisiae. Biochem. biophys. Acta 71: 227, 1963.
Rosenberg, T., Wilbrandt, W.:Uphill transport induced by counterflow. J. gen. Physiol. 41: 289, 1957.
Scharff, T. G., Kremer, E. H. III:A tentative mechanism for the anaerobic transport of glucose, fructose and mannose in yeast. Arch. Biochem. Biophys. 97: 192, 1962.
Stein, W.:Dimer formation and glucose transfer across the membrane of the red blood cell. Nature 191: 1277, 1961.
Wilbrandt, W.:The sugar transport across the red cell membrane. Symp. Membrane Transport and Metabolism. Publ. House Czechosl. Acad. Sci., Prague 1961.
Wilbrandt, W., Kotyk, A.:Transport of the sugar mono-and di-complexes in human erythrocytes. Naunyn-Schmiedebergs Arch. exp. Path. u. Pharm. 249: 279, 1964.
Wilbrandt, W., Rosenberg, T.:The concept of carrier transport and its corrolaries in pharmacology. Pharmacol. Rev. 13: 109, 1961.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kotyk, A. Properties of the sugar carrier in baker's yeast. Folia Microbiol 10, 30–35 (1965). https://doi.org/10.1007/BF02869838
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02869838