Abstract
The urinary excretion patterns of N-acetyl-β-D-glucosaminidase (NAG), alanine aminopeptidase (AAP) and protein/creatinine ratio (UP/UCR) were studied in 133 diabetic subjects under treatment, 7 patients with established diabetic nephropathy (DN) and 79 carefully selected (age-matched) healthy subjects. NAG, AAP and UP/UCR were highly elevated in DN, while in diabetics urinary NAG levels correlated well with the degree of long-term metabolic control indicated by glycosylated hemoglobin (GHB or Hba1). Both AAP and UP/UCR were found to be more sensitive than NAG, but less specific. Urinary NAG and AAP assays thus offer simple, sensitive and non-invasive techniques for prognostic indication of the onset of microangiopathic changes in long-term diabetic subjects.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Pinter, G.G. and Atkins, J.L. (1990). Lancet 335: 590.
D’Elia, J.A., Kaldamy, A., Miller, D.G., Albouriz, N.N. and Wienrauch, L.A. (1985). In Joslin’s Diabetes Mellitus, (Eds.. Marble, A, Krall, L.P. and Bradley R.F.) (Lea and Febiger, Philadelphia) p. 635.
Rasch, R. (1980). Diabetologia 18: 413.
Raab, W.P. (1972). Clin. Chem. 18: 5.
Belfiore, F., Napoli, E. and Vecchio, L.L. (1972). Diabetes 21: 1168.
Miller, B.F., Keyes, F.P. and Curreri, P.W. (1966). JAMA 195: 189.
Fushimi, H. and Tarui, S. (1976). Clin. Chim. Acta. 71: 1.
Whiting, P.H., Ross, I.S. and Borthwick, L. (1979) Clin. Chim. Acta. 92: 459.
Mogensen, C.E. (1976). Diabetes 25 (Suppl:2): 872.
SriKrishna, K., Pandey, A.P., Kirubakaran, M.G. and Kanagasabapathy, A.S. (1987). Clin. Chim. Acta. 163: 51.
SriKrishna, K., Sastry, J.C.M., Cherian, A.M. and Kanagasabapathy A.S. (1985). Asian. J. Clin. Sci. 5: 61.
SriKrishna, K., Kirubakaran, M.G., Pandey, A.P. and Kanagasabajpathy, A.S. (1985) Clin. Chim. Acta. 150: 69.
Srikrishna, K. and Kanagasabapathy, A.S. (1991). Ind. J. Clin. Biochem. 6: 17.
Nyberg, G., Blohme, G. and Norden, G. (1987). Diabetologia 30: 80.
Hostetter, T.H. (1986). Diabetic Nephropathy. (Eds. Brenner B.M. and Rector F.C.): The Kidney Vol. II, W.B. Saunders. Philadelphia, p. 1377.
Pinter, G.G., Stork, J.E., Wilson, P.D. and Fajer, A.B. (1983) Clin. Sci. 65: 393.
Pinter, G.G., Wilson, P.D. and Yuen, L.S.L. (1989). J. Physiol.) 417: 47
Scherberich, J.E. (1989). Clin. Chim. Acta. 185: 271.
Jung, K., Pergande, M., Schimke, E., Ratzmann, K.P. and Ilius, A (1988). Clin. Chem. 34: 544.
Jones, M.C. (1987). Int. Clin. Prod. Rev. 6: 64.
Shima, K., Hirota, M., Fukuda, M. and Tanaka, A. (1986) Clin. Chem. 32: 1818.
Beetham, R., Silver, A and Dawnay A. (1987). Clin. Chem. 33: 713.
Shimojo, N., Kitahashi, S., Naka, K., (1987). Metabolism 36: 277.
Severeni, G., Aliberti, L.M. and Girolamo, M.D. (1988) Clin. Chem. 34: 2430.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Srikrishna, K., Kanagasabapathy, A.S. & John, L. N-acetyl-β-D-glucosaminidase, alanine aminopeptidase and protein: Creatinine ratio as early indicators of diabetic microangiopathy. Indian J Clin Biochem 9, 5–8 (1994). https://doi.org/10.1007/BF02867843
Issue Date:
DOI: https://doi.org/10.1007/BF02867843