Abstract
The early effects of chromosomal manipulation of eggs and sperm on the yields of triploid and gynogenetic diploid larvae ofParalichthys olivaceus were investigated Triploidy was achieved by cold shocking fertilized eggs at 0–2°C for 45 minutes duration 5 minutes after, fertilization, and the induced triploidy rates were 31.2%–50% and the relative hatching rates were 53.3%–99%. Gynogenetic diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0–2°C for 45 minutes duration 5 minutes after fertilization. The induced gynogenetic diploid rates and the relative hatching rates were 94%–96% and 48.5%–68.5% respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the growth of the gynogenetic diploid experimental group larvae and the control group larvae according to Student’s t-test (α=0.05). The relationship between the early growth of the triploid experimental group larvae and that of gynogenetic diploid experimental group larvae was also studied.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Cherfas, N. B., Gomelsky, B., Ben-Dom, N.. et al., 1994. Assessment of triploid common carp (Cyprinus carpio L.) for culture.Aquac.127: 11–18.
Fast, A. W., Pewnim, T., Keawtabtim, R. et al., 1995. Comparative growth of diploid and triploid AsianClarias macrocephalus in Thailand.,J. World Aquac. Soc. 26: 390–395.
Felip, A., Zanuy, S., Carrillo, M. et al., 1999. Growth and gonadal development in triploid sea bass (Dicentrarchus labrax L.) during the first two years of age.Aquac.173: 389–399.
Fujioka, Y., 1998. Survival, growth and sex ratios of gynogenetic diploid honmoroko.J. Fish Biol.52: 430–442
Henken, A. M., Brunink, A. M., Richter, C. J. J., 1987. Differences in growth rate and feed utilization between diploid and triploid African catfishClarias gariepinus (Burchell 1822).Aquac.63: 233–242.
Ihseen, P. E., Mckay, L. R., Mcmillian, I. et al.., 1990. Ploidy manipulation and gynogenesis in fishes: cytogenetic and fisheries applications.Trans. Amer. Fish. Soc. 119: 698–717.
Liu Jing, You Feng, Wang Xincheng et al., 1999. Karyotype evidences of gynogesis in the left-eyed flounder,Paralichthys olivaceus (T. & S.).Oceanol. Limnol. Sin. 30(1): 68–72. (in Chinese with English abstract)
Lu, R., Chen, H., 1993. Advances in fish cell engineering in China.Aquac.111: 41–50.
Qin, J. G., Fast, A. W., Ako, H., 1998. Growout performance of diploid and triploid Chinese catfishClarias fuscus Aquac.166: 247–258.
Suzuki, R., Oshiro, T., Nakanishi, T., 1985. Survival, growth and fertility of gynogenetic diploids induced in the cyprinid loach,Nisgurnus anguillicadatus. Aquac. 48: 45–55.
Tabata, K., Gorie, S., 1988. Induction of gynogenetic diploid inParalichthys olivaceus by suppression of the 1st cleavage, with special reference to their survival and growth.Nippon Suisan Gakkaishi 54: 1867–1872
Wolters, W. R., Libay, G. S., Chriman, L., 1982. Effect of triploidy on growth and gonad development of channel catfish.Trans. Am. Fish. Soc.111: 102–125.
You Feng, Liu Jing, 1995. Karyotype evidences of triploidy in the left-eyed flounder,Paralichthys olivaceus (T. and S.).Oceanol. Limnol. Sin.,26(Supplement,5): 114–118. (in Chinese with English abstract)
Author information
Authors and Affiliations
Additional information
Contribution No. 3731 from the Institute of Oceanology, Chinese Academy of Sciences.
Project supported by the “China 863 Program” and Shandong Science and Technology Commission.
Rights and permissions
About this article
Cite this article
You, F., Liu, J., Wang, Xc. et al. Study on embryonic development and early growth of triploid and gynogenetic diploid left-eyed flounder,Paralichthys olivaceus (T. et S.). Chin. J. Ocean. Limnol. 19, 147–151 (2001). https://doi.org/10.1007/BF02863039
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02863039