Abstract
A predominant protein complex from the albumin fraction of potato (Solanum tuberosum L.) tuber has been isolated by size exclusion high performance liquid chromatography. The isolate has an estimated native molecular weight of 60 kD. Anionic gel electrophoresis of the isolate separated it into several polypeptides, all of which were glycosylated and had non-specific esterase activity. Two polypeptides between 35 and 42 kD were identified with SDS electrophoresis, thus the native form is postulated to be a dimer. The proteins in the isolate are antigenic to an antibody developed against ‘patatin,’ a tuber glycoprotein. Nutritional values for the isolate were extremely high, which suggests that it is at least partly responsible for the excellent nutritional value of potato protein.
Compendio
Mediante cromatografía líquida de exclusión de alta precisión (HPLC), se aisló de la fracción albuminoidea de tubérculos de papa (Solanum tuberosum L.) un complejo proteico predominante. El aislamiento tiene un peso molecular natural estimado de 60 kD. La electroforesis separó al aislamiento en varios polipectidos, todos ellos glicosilatados y con actividad de esterasa no específica. Por medio de electroforesis (SDS) se identificaron dos polipectidos, entre 35 y 42 kD, por lo que se considera que la forma natural del aislamiento es un dímero. Las proteínas del aislamiento son antigénicas a un anticuerpo desarrollado contra la’patatina,’ una glicoproteina del tubérculo. Los valores nutricionales del aislamiento fueron extremadamente altos, lo que sugiere que este complejo es por lo menos parcialmente responsable del excelente valor nutricional de la proteína de la papa.
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Scientific Journal Series Article #14634 of the Minnesota Experiment Station.
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Liedl, B.E., Kosier, T. & Desborough, S.L. HPLC isolation and nutritional value of a major tuber protein. American Potato Journal 64, 545–557 (1987). https://doi.org/10.1007/BF02853753
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DOI: https://doi.org/10.1007/BF02853753