Abstract
A strong relationship was found between membrane permeability and starch to sugar conversion in stored potatoes. Tissue from tubers stored at low temperature had higher sugar content and more permeable membranes than from that of tubers stored at high temperature. Tubers, moisture stressed during growth and stored at 5.5 C, accumulated more reducing sugars and had more permeable membranes than tubers from normally irrigated plants when stored at the same temperature. The basal portion of Russet Burbank tubers accumulated more sugars and was also found to have significantly more permeable membranes than the apical portion. Examination of membrane order with spin-labeled lipid probes showed that the order parameter of amyloplast membranes was less at low temperatures than when measured at higher temperatures. Membranes examined with spin-labeled lipid probes from tubers stored at 5.5 C and 15.5 C showed similar order parameters at all temperatures measured between 0 and 25 C with a linear decrease in S values at higher temperatures. These results strongly indicate a physical change of membranes with changing temperature, thus influencing sugar accumulation, which is also reversible, as indicated by loss of sugars during the reconditioning process.
Resumen
Una fuerte relación fue encontrada entre la permeabilidad de la membrana y la conversión de almidón a azúcares en papas almacenadas. Tejidos de tubérculos almacenados a bajas temperaturas, tuvieron mayor contenido de azúcares y más membranas perm≸bles que aquellos tubérculos almacenados a altas temperaturas. Tubérculos sometidos a stress de humedad durante el crecimiento y almacenados a 5.5°C acumularon más azúcares reductores y tuvieron más membranas permeables que los tubérculos regados normalmente y almacenados a la misma temperature.
La porción basal de tubérculos Russet Burbank, acumuló más azúcares y se le encontró también poseer significativamente más membranas permeables que en la porción apical. Un exámen del orden de la membranas, con ensayos de lipidos spin-labeled mostró que el parámetro de orden de membranas de amiloplastos fue menor a bajas temperaturas que cuando era medido a altas temperaturas. Las membranas examinadas de tubérculos almacenados a 5.5°C y 15.5°C bajo pruebas de lípidos spin-labeled mostraron parámetros de orden similares a todas las temperaturas medidas entre 0 y 25°C con un descenso linear de valores S a altas temperaturas. Estos resultados indican fuertemente un cambio físico de las membranas con la temperatura, influenciando así la acumulación de azucares que es también reversible, tal como indica la pérdida de azúcares durante el proceso de recondicionamiento.
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Literature Cited
Arreguin-Lozano, B. and J. Bonner. 1949. Experiments on sucrose formation by potato tubers as influenced by temperature. Plant Physiol 24:720–738.
Crafts, C.C. 1967. Respiration of potato tissue as influenced by previous storage temperatures of the tubers. Am Potato J 44:174–181.
Glinka, G. and L. Reinhold. 1971. Abscisic acid raises the permeability of plant cells to water. Plant Physiol 48:103–105.
Griffith, O.H. and P.C. Jost. 1976. Lipid spin labels in biological membranes.In: L.J. Berlinger, ed., Spin Labeling Theory and Application. Academic Press, New York, pp. 453–523.
Iritani, W.M. and L. Weller. 1971. The development of translucent end tubers. Am Potato J 50:223–233.
Iritani, W.M., L. Weller and P.S. Russell. 1973. Relative differences in sugar content of basal and apical portions of Russet Burbank potatoes. Am Potato J 50:24–31.
Iritani, W.M. and L. Weller. The influence of low fertility and vine killing on sugar development in apical and basal portions of Russet Burbank potatoes. Am Potato J (in press).
Isherwood, F.A. 1973. Starch-sugar interconversion inSolanum tuberosum. Phytochemistry 12:2579–2591.
Isherwood, F.A. and M.G.H. Kennedy. 1975. The composition of the expressed sap from cold stored potatoes. Phytochemistry 14:83–84.
Isherwood, F.A. 1976. Mechanism of starch-sugar interconversion inSolanum tuberosum. Phytochemistry 15:33–41.
Jones, P.C.T. 1970. The effect of light, temperature and anaesthetics on ATP levels in the leaves ofChemopodium rubrum andPhaseolus vulgaris. J Exp Bot 21:58–63.
Kennedy, M.G.H. and F.A. Isherwood. 1975. Activity of phosphorylase inSolanum tuberosum during low temperature storage. Phytochemistry 14:667–670.
Lyons, J.M. 1973. Chilling injury in plants. Annu Rev Plant Physiol 24:445–466.
McKersie, B.D. and J.E. Thompson. 1977. Lipid crystallization in senescent membranes from cotyledons. Plant Physiol 59:803–807.
Ohad, I., I. Friedberg, A. Ne’eman and M. Schramm. 1971. Biogenesis during maturation and storage of potato tubers. Plant Physiol 47:465–477.
Pollock, C. and T. Ap Rees. 1975. Activities of enzymes of sugar metabolism in cold stored tubers ofSolanum tuberosum. Phytochemistry 14:613–617.
Poovaiah, B.W., Y. Mizrahi, H.C. Dostal, J.H. Cherry and A.C. Leopold. 1975. Water permeability during tomato fruit development in normal and in non-ripening mutant. Plant Physiol 56:813–815.
Pressey, R. 1970. Changes in sucrose synthetase and sucrose phosphate synthetase activities during storage of potatoes. Am Potato J 47:245–251.
Raison, J.R., J.M. Lyons, R.J. Mehlhorn and A.D. Keith. 1971. Temperature-induced phase changes in mitochondria membranes detected by spin labeling. J Biol Chem 246:4036–4040.
Raison, J.R., J.M. Lyons and W.W. Thomson. 1971. The influence of membranes on the temperature-induced changes in the kinetics of some respiratory enzymes of mitochondria. Arch Biochem Biophys 142:83–90.
Razin, S., A. Ne’eman and I. Ohad. 1969. Selective reaggregation of solubilized mycoplasma-membrane proteins and the kinetics of membrane reformation. Biochem Biophys Acta 193:277–293.
Shekhar, V.C. and W.M. Iritani. Starch-sugar interconversion inSolanum tuberosum. I. Influence of inorganic ions. Am Potato J (in press).
Sowokinos, J.R. 1971. Relationship of sucrose synthetase cleavage activity to the chemical and physical maturity of Norchip and Kennebec potatoes. Am Potato J 48:37–46.
Thimann, K.V. and E.W. Samuel. 1955. The permeability of potato tissue to water. Proc Nat Acad Sci USA 41:1029–1033.
Weist, S.C. and P.L. Steponkus. 1977. Accumulation of sugars and plasmalemma alterations. Factors related to the lack of cold acclimation in young roots. J Am Soc Hortic Sci 102:119–123.
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Scientific Paper No. 5018. College of Agriculture Research Center, Washington State University, Pullman. Project No. 1949. Supported in part by a grant from the following processors: Carnation Company, Rogers Walla Walla, Pride Pak Food, Chef Reddy, and Twin City Foods, Incorporated. This paper was written in partial fulfillment for a Ph.D. degree.
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Shekhar, V.C., Iritani, W.M. & Magnuson, J. Starch-sugar interconversion inSolanum tuberosum L. II. Influence of membrane permeability and fluidity. American Potato Journal 56, 225–235 (1979). https://doi.org/10.1007/BF02853369
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DOI: https://doi.org/10.1007/BF02853369