Abstract
Phenolic compounds in Type A glandular trichomes of two insectresistant potato species were separated and identified using a combination of high pressure liquid chromatography (HPLC), UV-visible spectrometry, and1H proton nuclear magnetic resonance (NMR) procedures. Type A trichomes ofSolanum berthaultii andS. polyadenium contained a phenolic compound tentatively identified as the glucose ester of p-hydroxyphenylpropionic acid. Its presence in trichomes of both species suggests that this compound may play a major role in formation of the brownish, insect entrapping exudate characteristic of trichome-mediated insect resistance in these species. Chlorogenic acid, a major phenolic constituent of Type A trichomes ofSolanum polyadenium was not detected in trichomes of 5.berthaultii. The exclusive presence of chlorogenic acid in trichome exudates ofS. polyadenium may be responsible, at least in part, for the greater levels of insect entrapment by this species, compared with that ofS. berthaultii.
Resumen
Se separaron e identificaron compuestos fenólicos en los tricomas glandulares Tipo A de dos especies de papa resistentes a insectos, utilizando una combinación de los métodos de cromatografía líquida de alta presión (HPLC), espectrometria visible UV, y la resonancia nuclear magnética del protón1H, (NMR). Los tricomas Tipo A deSolanum berthaultii y S. polyadenium contenían un compuesto fenólico tentativamente identificado como el ester glucosado del ácido p-hidroxifenilpropiónico. Su presencia en los tricomas de ambas especies sugiere que este compuesto puede jugar un importante rol en la formación del exudado parduzco que entrampa a los insectos y que es característico de la resistencia en estas dos especies, ejercida sobre los insectos atrapados en sus tricomas. El ácido clorogénico, un constituyente principal de los tricomas Tipo A deSolanum polyadenium no fue detectado en los tricomas deSolanum berthaultii. La presencia exclusiva del ácido clorogénico en los exudados de los tricomas deSolanum polyadenium puede ser responsable, al menos en parte, de los mayores niveles de entrampamiento encontrados en esta especie, en comparación con los observados conS. berthaultii.
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A publication of the Cornell University Agricultural Experiment Station, New York State College of Agriculture and Life Sciences, a Statutory College of S.U.N.Y.
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Avé, D.A., Tingey, W.M. Phenolic constituents of glandular trichomes onSolanum berthaultii andS. polyadenium . American Potato Journal 63, 473–480 (1986). https://doi.org/10.1007/BF02852942
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DOI: https://doi.org/10.1007/BF02852942