Abstract
Abscisic acid (ABA), sucrose, reducing sugars and proline contents were monitored over an eleven-month period in tubers of three potato cultivars that varied in tuber dormancy. ABA and proline levels increased after top pulling the haulms and were not affected by harvest date. Proline did not change in either a storage temperature or cultivar-specific manner. The highest concentrations of ABA were found in tubers stored at 2°C while the lowest concentrations occurred in tubers stored at 20°C. At 10°C, the end of tuber dormancy in the cultivars Kennebec and Nooksack (but not Sebago) coincided with the decline in ABA content. There was no evidence of a threshold concentration of ABA below which sprouting would occur. Tuber samples of 10 different cultivars were removed from 10°C and placed in 20°C storage (in darkness). Initial ABA concentrations (i.e., at the time of removal from 10°C storage) were positively correlated with duration of dormancy and negatively correlated with subsequent rates of sprout elongation at 20°C. Sucrose content was negatively correlated with duration of dormancy. Reducing sugars responded primarily to storage temperature and did not appear to be related to dormancy or sprouting.
Resumen
El contenido de ácido abscisico (ABA) sucrosa, azúcares reductores y prolina fueron manipulados por un periodo de 11 meses en tubérculos de 3 variedades de papa que mostraban una considerable variatión en dormancia del tubérculo. Los niveles de ABA y prolina se incrementaron después de defoliar la parte superior de la planta y no fueron afectadas por la fecha de cosecha. El contenido de prolina no cambió al variar ya sea la temperatura de almacenamiento o el cultivar. Las concentraciones más altas de ABA fueron encontradas en tubérculos almacenados a 2°C mientras que las concentraciones más bajas ocurrieron en tubérculos almacenados a 20°C. A 10°C el término de la dormancia en los tubérculos en los cultivares Kennebec & Nooksack (no Sebago) coincidió con disminución en el contenido de ABA. No hubo evidencia de una concentración minima de ABA por debajo de la cual ocurriría brotación de los tubérculos Muestras de tubérculos de 10 diferentes cultivares fueron llevadas de 10°C a 20°C de temperatura de almacenamiento (en oscuridad). Las concentraciones iniciales de ABA fueron correlacionadas positivamente con la duración del período de dormancia y negativamente con las tasas subsecuentes de alargamiento de brotes a 20°C. El contenido de sucrosa mostró correlación negativa con la duración de la dormancia. Los azúcares reductores fueron principalmente afectadas por la temperatura de almacenamiento y aparentemente no mostraron relación con la dormancia o brotación.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Literature Cited
Bailey, K.M., I.D.J. Phillips and D. Pitt. 1978. The role of buds and gibberellin in dormancy and the mobilization of reserve materials in potato tubers. Ann Bot 42:649–657.
Bates, L.S., R.P. Waldren and I.D. Teare. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39:205–207.
Blumenthal-Goldschmidt, S. and L. Rappaport. 1965. Regulation of bud rest in tubers of potato,Solanum tuberosum L. II. Inhibition of sprouting by inhibitor β complex and reversal by gibberellin A0. Plant and Cell Physiol 6:601–608.
Burton, W.G. 1965. The sugar balance in some British potato varieties during storage. I. Preliminary observations. Eur Potato J 8:80–91.
Cottle, W. and P.E. Kolattukudy. 1982. Abscisic acid stimulation of suberization: Induction of enzymes and deposition of polymeric components and associated waxes in tissue cultures of potato tuber. Plant Physiol 70:775–780.
El-Antably, H.M.M., P.F. Wareing and J. Hillman. 1967. Some physiological responses to d,1-abscisin (dormin). Planta (Berl) 73:74–90.
Franklin, J. and T. Hemberg. 1980. Seasonal changes of the different components of the inhibitor complex in potato tubers. Physiol Plant 50:227–232.
Hartmans, K. J. and A. Van Es. 1979. The influence of growth regulators GA3, ABA, kinetin and IAA on sprout and root growth and plant development using excised potato buds. Potato Res 22:319–332.
Hemberg, T. 1947. Studies of auxins and growth inhibiting substances in the potato tuber and their significance with regard to its rest period. Acta Horti Bergiani 14: 133–220.
Hemberg, T. 1949. Significance of inhibiting substances and auxins for the rest period of the potato tuber. Physiol Plant 2:24–36.
Hemberg, T. 1952. The significance of the acid growth-inhibiting substances for the restperiod of the potato tuber. Physiol Plant 5:115–129.
Hemberg, T. 1958. The significance of the inhibitor β complex in the rest period of the potato tuber. Physiol Plant 11:615–626.
Hemberg, T. 1965. The significance of inhibitors and other chemical factors of plant origin in the induction and breaking of rest periods. Handb Pflanzenphysiol 15:669–698.
Holst, U.-B. 1971. Some properties of inhibitor fromSolanum tuberosum compared to abscisic acid. Physiol Plant 24:392–396.
Huber, W. 1974. Uber den EnfluB von NaCl-oder Abscisinsaure-Behandlung auf den Proteinmetabolismus und einige weitere Enzyme des Aminosaurestoffwechsels in Keimlingen vonPennisetum typhoides. Planta 121:225–235.
Korableva, N.P., K.A. Karavaeva and L.V. Metlitskii. 1980. Changes of abscisic acid content in potato tuber tissues in the period of deep dormancy and during germination. Fiziol Rast 27:585–591.
Li, C.C. 1975. Path analysis—a primer. The Boxwood Press, Pacific Grove, California.
Lindsay, H. 1973. A colorimetric estimation of reducing sugars in potatoes with 3,5-dinitrosalicyclic acid. Potato Res 16:176–179.
Milborrow, B.V. 1972. Stereochemical aspects of the formation of double bonds in abscisic acid. Biochem J 128:1135–1146.
Miller, L.P. 1933. Effect of various chemicals on the sugar content, respiratory rate and dormancy of potato tubers. Contrib Boyce Thompson Inst. 5:213–234.
Rajagopal, V. and A. Skytt-Andersen. 1978. Does abscisic acid influence proline accumulation in stressed leaves? Planta 143:85–88.
Siegel, S. 1956. Nonparametric statistics. McGraw-Hill, New York.
Soliday, C.L., B.B. Dean and P.E. Kolattukudy. 1978. Suberization: inhibition by washing and stimulation by abscisic acid in potato disks and tissue culture. Plant Physiol 61:170–174.
Sowokinos, J.R. 1978. Relationship of harvest sucrose content to processing maturity and storage life of potatoes. Am Potato J 55:333–344.
Van Es, A. and K.J. Hartmans. 1969. The influence of abscisin II and gibberellic acid on the sprouting of excised potato buds. Eur Potato J 12:59–63.
Wurr, D.C.E. 1978a. Studies of the measurement and interpretation of potato sprout growth. J Agric Sci Camb 90:335–340.
Wurr, D.C.E. 1978b. ‘Seed’ tuber production and management.In: P.M. Harris (Ed.), The potato crop, the scientific basis for improvement, pp. 327–354. Chapman & Hall, London.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Coleman, W.K., King, R.R. Changes in endogenous abscisic acid, soluble sugars and proline levels during tuber dormancy inSolanum tuberosum L.. American Potato Journal 61, 437–449 (1984). https://doi.org/10.1007/BF02852813
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02852813