Abstract
A diagnostic procedure for potato spindle tuber disease is described which allows detection of the causal viroid in small amounts of potato and tomato tissue. The method involves extraction of cellular nucleic acids, their separation by polyacrylamide gel electrophoresis and staining with toluidine blue O. The procedure has been used to index greenhouse and field potatoes for mild and severe strains of the pathogen
Resumen
Un procedimiento para diagnosticar la enfermedad del “tubérculo ahusado” (PSTV) es descrito, el cual permite detectar el viroide cau sante en pequenas cantidades del tejido de la papa y del tomate. Los métodos incluyen extracción de ácidos nucléicos celulares, su separación por geles de polyacrylamida, electroforesis y tincción con azul de toluideno O.
El prodedimiento ha sido usado para indexar papas tanto en el invernadero como en el campo para los “strains” sauve y severo del pa tógeno
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Morris, T.J., Wright, N.S. Detection on polyacrylamide gel of a diagnostic nucleic acid from tissue infected with potato spindle tuber viroid. American Potato Journal 52, 57–63 (1975). https://doi.org/10.1007/BF02852039
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DOI: https://doi.org/10.1007/BF02852039