Abstract
This study on dynamic changes of culture color, astaxanthin and chlorophylls, inorganic N including N−NO −3 , N−NO −2 and N−NH +4 in batch culture ofHaematococcus pluvialis exposed to different additive nitrate concentration showed (1) ast/chl ratio was over 0.8 for brown and red algae, but was usually less than 0.5 for green and yellow algae; (2) N−NO −3 , in general, was unstable and decreased, except for a small unexpected increase in nitrate enriched treatment groups; (3) measurable amounts of N−NO −2 , and N−NH +4 were observed respectively with three change modes although no external nitrite and ammonia were added into the culture; (4) a non-linear correlation between ast/chl ratio (or color) changes and the levels of N−NO −3 , N−NO −2 , N−NH +4 inH. pluvialis culture; (5) up and down variation of the ast/chl ratio occurred simultaneously with a perceptible color change from yellow to brown (or red) when N−NO −3 , N−NO −2 and N−NH +4 fluctuated around 30, 5, 5, μmol/L respectively; (6) existence of three dynamic modes of N−NO −3 , N−NO −2 and N−NH +4 changes, obviously associated with initial external nitrate; (7) the key level of total inorganic N concentration regulating the above physiological changes during indoor cultivation was about 50 μmol/L; and (8) 0.5–10 mmol/L of nitrate was theoretically conducive to cell growth in batch culture.
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Contribution No. 4139 from Institute of Oceanology, Chinese Academy of Sciences.
Projects 39500114, 39970575, A/2786-1 and 9565 supported by NSFC, IFS (International Foundation for Sciences) and Sci-Tech Commission of Shandong Province respectively.
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Jian-guo, L., Ming-yan, Y., Jing-pu, Z. et al. Dynamic changes of inorganic nitrogen and astaxanthin accumulation inHaematococcus pluvialis . Chin. J. Ocean. Limnol. 20, 358–364 (2002). https://doi.org/10.1007/BF02847927
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DOI: https://doi.org/10.1007/BF02847927