Abstract
An alternative pretreatment method for mitotic chromosomes in potato root tips is described. Root tips were treated with a synthetic pyrethroid, a permethrine, {3-Phenoxybenzil (±) -Cis, trans, 3-(2,2 dichlorovinyl) -2,2-dimethyl ciclopropane carboxylate}. with concentrations of 10ppm, 100ppm, 200ppm, and 500ppm. Cold water and 0.02M 8-hydroxyquinoline were used for comparison at 4 C. Mitotic index was obtained for all treatments by using a standard squash method, and pretreatment with 100ppm of the pyrethroid demonstrated a large number of mitotic cells and well condensed chromosomes for a comprehensive observation in mitotic cells
Compendio
Se presenta una tecnica alternativa para tratar chromosomas mitoticos como pretratamiento en raices de papas. Raices se trataron con pyrethroides syntheticos, una permethrina, (carboxilato ciclopropano-3- Fenoxibencilo (±)Cis trans-2,2-dimetilo 3-(2,2-diclorovinila) en concentracion de 10ppm, 100ppm, 200ppm, y 500ppm. Agua helada y 8-hydroxyquinolina 0.02M se emplearon como controles para comparar los diferentes tratamientos con pyrethroides a una temperatura de 4C. El indice mitotico se determinó por el method microscopico establecído para preparar muestras de puntas de raices. Con el pretratamiento con 100ppm de pyrethroide a 100ppm se encontró un gran numero de celulas mitoticas y chromosomas bien observables.
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Literature Cited
Arumuganathan, K., J.P. Slattery, S.D. Tanksley and E. Earle. 1991. Preparation and flow cytometric analysis of metaphase chromosomes of tomato. Theor Appl Genet 82:101–111.
Chayen, J. and U.J. Miles. 1954. Preparing plant root tip cells for microscopical examination. Stain Technol 29:33–39.
Klein, M. 1990. C-mitotic action of the insecticide Ambush 25EC inAllium cepa L. Genetica Polonica 31(2):107–113.
Pijnacker, L.P. and M.A. Ferwerda. 1984. Giemsa C-banding of potato chromosomes. Can J Genet Cytol 26:415–419.
Visser, R.G., R. Hoekstra, F.R. van der Leij, L.P. Pijnacker, B. Witholt and W.J. Feenstra1988. In situ hybridization to somatic metaphase chromosomes of potato. Theor Appl Genet 76:420–424.
Watanabe, K. 1991. Present status of germplasm enhancement at CIP and application of biotechnology.In: Dodds, J.H. (Ed.), Report of the Planning Conference on Application of Molecular Techniques to Potato Germplasm Enhancement, International Potato Center, Apartado 5969, Lima, Peru, 135–140pp.
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Watanabe, K.N., Orrillo, M. An alternative pretreatment method for mitotic chromosome observation in potatoes. American Potato Journal 70, 543–548 (1993). https://doi.org/10.1007/BF02846755
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DOI: https://doi.org/10.1007/BF02846755