Abstract
Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mitochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denaturation, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology, biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed. New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis, flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.
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Babiak, I., J. Glogowski, K. Goryczko, S. Dobosz, H. Kuzminski, J. Strzezek I and W. Demianowicz, 2001. Effect of extender composition and equilibration time on fertilization ability and enzymatic activity of rainbow trout cryopreserved spermatozoa.Theriogenology 56: 177–192.
Billard, R., 1983. Ultrastructure of trout spermatozoa: changes after dilution and deep-freezing.Cell Tissue Res. 228: 205–218.
Blaxter, J. H. S., 1953. Sperm storage and cross-ertilization of spring and autumn spawning herring.Nature 172: 1189–1190.
Chen, S. L., X. S. Ji, G. C. Yu, Y. S. Tian and Z. X. Sha, 2004. Cryopreservation of sperm from turbot (Scophthalmus maximus, and application to large-scale fertilization.Aquaculture 236: 547–556.
Ciereszko, A. and K. Dabrowski, 1994. Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage.Fish Physiol. Biochem. 12: 357–367.
Conget, P., M. Fernández, G. Herrera and J. J. Minguell, 1996. Cryopreservation of rainbow trout (Oncorhynchus mykiss) spermatozoa using programmable freezing.Aquaculture 143: 319–329.
Dreanno, C., M. Suquet, L. Quemener, J. Cosson, F. Fierville, Y. Normant and R. Billard, 1997. Cryopreservation of turbot (Scophthalmus Maximus) spermatozoa.Theriogenology 48: 589–603.
Drokin, S., H. Stein and H. Bartscherer, 1998. Effect of cryopreservation on the fine structure of spermatozoa of rainbow trout (oncorhynchus mykiss) and brown trout (salmo trutta F. fario).Cryobiology 37: 263–270.
Duke, R. C. and J. J. Cohen, 1986. Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolisis.Proc. Natl. Acad. Sci. 80: 61–63.
Evenson, D. P., L. K. Jost, D. Marshall, M. J. Zinaman, E. Clegg, K. Purvise, P. De Angelis and O. P. Claussen, 1999. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum.Reprod. 14: 1039–1049.
Fabbrocini, A. S. L. Lavadera, S. Rispoli and G. Sansone, 2000. Cryopreservation of Seabream (Sparus aurata) Spermatozoa.Cryobiology 40: 46–53.
Garner, D. L., D. Pinkel, L. A. Johnson and M. M. Pace, 1986. Assessment of spermatozoal function using dual fluorescent staining and flow cytometric analysis.Biol. Reprod. 34: 127–138.
Garner, D. L. and L. A. Johnson, 1995. Viability assessment of mammalian sperm using SYBR-14 and propidium iodide.Biol. Reprod. 53: 276–284.
Glogowski, J., I. Babiak, K. Goryczko and S. Dobosz, 1996. Activity of aspartate aminotransferase and acid phosphatase in cryopreserved trout sperm.Reprod. Fertil. Dev. 8: 1179–1184.
Graham, J. K., E. Kunze and R. H. Hammerstedt, 1990. Analysis of sperm cell viability, acrosomal integrity and mitochondrial function using flow cytometry.Biol. Reprod. 43: 55–64.
Gravance, C.G., D.L. Garner, M. G. Miller and T. Berger, 2001. Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function.Reprod Toxico. 15: 5–10.
Grout, B. W. W. and G. J. Morris, 1987. The Effect of Low Temperature on Biological Stem. Edware Amonld, Cambridge, UK.
Gwo, J. C. and C. R. Arnold, 1992. Cryopreservation of Atlantic croaker spermatozoa: evaluation of morpho-logical changes.J. Exp. Zool. 264: 444–453.
Gwo, J. C., H. Ohta, K. Okuzawa and H. C. Wu, 1999. Cryopreservation of sperm from the endangered Formosan landlocked salmon (Oncorhynchus masou formosanus).Theriogenology 51: 569–582.
Gwo, J. C., C. Y. Wu, W. S. Chang and H. Y. Cheng, 2003. Evaluation of damage in Pacific oyster (Crassostrea gigas) spermatozoa before and after cryopreservation using comet assay.Cryo. Letter 24: 171–180.
Homma-Takeda, S., Y. Kugenuma, T. Iwamuro, Y. Kumagai and N. Shimojo, 2001. Impairment of spermatogenesis in rats by methylmercury: involvement of stage- and cell-specific germ cell apoptotis.Toxicology 169: 25–35.
Hughes, C. M., J. Valerie McKelvey-Martin and Sheena E. M. Lewis, 1999. Human sperm DNA integrity assessed by the Comet and ELISA assays.Mutagenesis 14: 71–75.
Kavak, A., A. Johannisson, N. Lundeheim, H. Rodriguez-Martinez, M. Aidnik and S. Einarsson, 2003. Evaluation of cryopreserved stallion semen from Tori and Estonian breeds using CASA and flow cytometry.Anim. Reprod. Sci. 76: 205–216.
Kime, D.E., M. Ebrahimi, K. Nysten, I. Roelants, E. Rurangwa, H. D. M. Moore and F. Ollevier, 1996. Use of computer assisted sperm analysis CASA for monitoring of the effects of pollution on sperm quality of fish; application to the effects of heavy metals.Aquat. Toxicol. 36: 223–237.
Labbe, C., A. Martoriati, A. Devaux and G. Maisse, 2001. Effect of sperm cryopreservation on sperm DNA stability and progeny development in rainbow trout.Mol. Reprod. Dev. 60: 397–404.
Lahnsteiner, F., T. Weismann and R. A. Patzner, 1992. Fine structural changes in spermatozoa of the grayling,Thymallus thymallus (Pisces: Teleostei), during routine cryopreservation.Aquaculture 103: 73–84.
Lahnsteiner, F., B. Berger, T. Wiesmann and R. Patzner, 1996a. Changes in morphology, physiology, metabolism, and fertilization capacity of rainbow trout semen following cryopreservation.Prog. Fish. Cult. 58: 149–159.
Lahnsteiner, F., B. Berger, T. Weismann and R. Patzner, 1996b. Fine structure and motility of spermatozoa and composition of the seminal plasma in the perch.J. Fish Biol. 47: 492–508.
Lahnsteiner, F., B. Berger, T. Weismann and R. Patzner, 1996c. Physiological and biochemical determination of rainbow trout,Oncorhynchus mykiss semen quality for cryopreservation.J. Appl. Aquacult. 6: 47–73.
Lahnsteiner, F., T. Weismann, R. A. Patzner, 1997. Aging processes of rainbow trout semen during storage.Prog. Fish Cult. 59: 272–279.
Lahnsteiner, F., B. Berger, T. Weismann and R. A. Patzner, 1998. Determination of semen quality of the rainbow trout,Oncorhyn-chus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolism.Aquaculture 163: 163–181.
Lahnsteiner, F., B. Berger, A. Horvath, B. Urbanyi and T. Weismann, 2000. Cryopresevation of spermatozoa in cyprindid fishes.Theriogenology 54: 1477–1496.
Love, C. C., J. A. Thompson, S. P. Brinsko, S. L. Rigby, T. L. Blanchard, V. K. Lowry and D. D. Varner, 2003. Relationship between stallion sperm motility and viability as detected by two fluorescence staining technique using flow cytometry.Theriogenology 60: 1127–1138.
Mazur, P., 1970. Cryobiology: the freezing of biological systems,Science 168: 939–949.
Mazur, P. and N. Rigopoulos, 1983. Contributions of unfrozen fraction and of salt concentration to the survival of slowly frozen human erythrocytes: influence of warming rate.Cryobiology 20: 274–289.
McNiven, M. A., R. K. Gallant and G. F. Richardsonb, 1992. In vitro methods of assessing the viability of rainbow trout spermatozoa.Theriogenology 38: 679–686.
Ogier de Baulny, B., Y. Le Vern, D. Kerboeuf and G. Maisse, 1997. Flow cytometric evaluation of mitochondrial activity and membrane integrity in fresh and cryopreserved rainbow trout (Oncorhynchus mykiss) spermatozoa.Cryobiology 34: 141–149.
Ogier de Baulny, B., C. Labbé and G. Maisse, 1999. Membrane integrity, mitochondrial activity, ATP content, and motility of the European catfish (Silurus glanis) testicular spermatozoa after freezing with different Cryoprotectants.Cryobiology 39: 177–184.
Papaioannou, K. Z., R. P. Murphy, R. S. Monks, N. Hynes, M. P. Ryan, M. P. Boland and J. E. Roche, 1997. Assessment of viability and mitochondrial function of equine spermatozoa using double staining and flow cytometry.Theriogenology 48: 299–312.
Papaioannou, K.Z., R. P. Murphy, R. S. Monks, N. Hynes, M. P. Ryan, M. P. Boland and J. F. Roche, 1997. Assessment of viability and mitochondrial function of equine spermatozoa using double staining and flow cytometry.Theriogenology 48: 299–12.
Sakkas, D., E. Mariethoz, G. Manicardi, D. Bizzarro, P. G. Bianchi and U. Bianchi, 1999. Origin of DNA damage in ejaculated spermatozoa,Rev. Reprod. 4: 31–37.
Segovia, M., J. A. Jenkins, C. Paniagua-Chavez and T. R. Tiersch, 2000. Flow cytometric evaluation of antibiotic effects on viability and mitochondrial function of refrigerated spermatozoa of Nile Tilapia.Theriogenology 53: 1489–1499.
Sun Y., K. D. Clinkebeard, C. Clarke, L. Cudd, S. K. Highlander and S. M. Dabo, 1999.Pasteurella haemolytica leukotoxin induced apoptosis of bovine lymphocytes involves DNA fragmentation.Vet. Microbiol. 65: 153–66.
Sun, J. G., Jurisicova, A. and R. F. Casper, 1997. X-ray-induced DNA double-strand breaks in human sperm.Mutagenesis 13: 75–79.
Wamecke, D. and H. J. Pluta, 2003. Motility and fertilizing capacity of frozen/thawed common carp (Cyprinus carpio L.) sperm using dimethyl-acetamide as the main cryoprotectant.Aquaculture 215: 167–185.
Yao, Z., L. W. Crim, G. F. Richardson and C. J. Emerson, 2000. Motility, fertility and ultrastructural changes of ocean pout (Macrozoarces americanus L.) sperm after cryopreservation.Aquaculture 181: 361–375.
Zhang, Y. Z., S. C. Zhang, X. Z. Liu, Y. Y. Xu, C. L. Wang, M. S. Sawant, J. Li and S. L. Chen, 2003. Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology.Theriogenology 60: 989–996.
Zilli, L., R. Schiavone, V. Zonno, C. Storelli and S. Vilella, 2003. Evaluation of DNA damage inDicentrarchus labrax sperm following cryopreservation.Cryobiology 47: 227–235.
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Funded by the National High Technology R&D Program of China (863 Program, Nos. 2001AA621100, 2003AA603510 and 2004AA603310).
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Jun, L., Qinghua, L. & Shicui, Z. Evaluation of the damage in fish spermatozoa cryopreservation. Chin. J. Ocean. Limnol. 24, 370–377 (2006). https://doi.org/10.1007/BF02842852
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DOI: https://doi.org/10.1007/BF02842852