Abstract
Lectins are proteins that specifically bind to a particular carbohydrate structure. Affinity chromatography with immobilized lectins is a quite effective technique not only for the fractionation of glycoproteins or oligosaccharides but also their structural assessment. In this article, we focus on the separation of glycopeptides and oligosaccharides derived from glycoproteins by affinity chromatography on immobilized lectin columns.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Kornfeld, R. and Kornfeld, S. (1985) Assembly of asparagine-linked oligosaccharides.Annu. Rev. Biochem. 54, 631–664.
Tsuji, T., Irimura, T., and Osawa, T. (1981) The carbohydrate moiety of Band 3 glycoprotein of human erythrocyte membrane.J. Biol. Chem. 256, 10,497–10,502.
Fukuda, M., Dell, A., Oates, J. E., and Fukuda, M. N. (1984) Structure of branched lactosaminoglycan, the carbohydrate moiety of Band 3 isolated from adult human erythrocytes.J. Biol. Chem. 259, 8260–8273.
Merkle, R. K. and Cummings, R. D. (1987) Relationship of the terminal sequences to the length of poly-N-acetyllactosamine chains in asparagine-linked oligosaccharides from the mouse lymphoma cell line BW5147.J. Biol. Chem. 282, 8179–8189.
Fukuda, M. (1985) Cell surface glycoconjugates as onco-differentiation markers in hematopoietic cells.Biochem. Biophys. Acta. 780, 119–150.
Fukuda, M., Kondo, T., and Osawa, T. (1976) Studies on the hydrazinolysis of glycoproteins. Core structures of oligosaccharides obtained from porcine thyroglobulin and pineapple stem bromelain.J. Biochem. 80, 1223–1232.
Takasaki, S. and Kobata, A. (1978). Microdetermination of sugar composition by radioisotope labeling.Methods Enzymol. 50, 554.
Tai, T., Yamashita, K., Ogata, M. A., Koide, N., Muramatsu, T., Iwashita, S., Inoue, Y., and Kobata, A. (1975) Structural studies of two ovalbumin glycopeptides in relation to the endo-β-N-acetylglucos-aminidase specificity.J. Biol. Chem. 250, 8569–8575.
Rupley, J. A. (1964) The hydrolysis of chitin by concentrated hydrochloric acid, and the preparation of low-molecular-weight substrates for lysozyme.Biochem. Biophys. Acta. 83, 245–255.
Krusius, T., Finne, J., and Rauvala, H. (1978) The poly(glycosyl) chains of glycoproteins. Characterization of a novel type of glycoprotein saccharides from human erythrocyte membrane.Eur. J. Biochem. 92, 289–300.
Yamamoto, K., Tsuji, T., Tarutani, O., and Osawa, T. (1984) Structural changes of carbohydrate chains of human thyroglobulin accompanying malignant transformations of thyroid glands.Eur. J. Biochem. 143, 133–144.
Tsuji, T., Irimura, T., and Osawa, T. (1980) The carbohydrate moiety of Band-3 glycoprotein of human erythrocyte membranes.Biochem. J. 187, 677–686.
Baenziger, J. U. and Fiete, D. (1979) Structural determinants of Ricinus communis agglutinin and toxin specificity for oligosaccharides.J. Biol. Chem. 254, 9795–9799.
Irimura, T., Tsuji, T., Yamamoto, K., Tagami, S., and Osawa, T. (1981) Structure of a complex-type sugar chain of human glycophorin A.Biochemistry 20, 560–566.
Shibuya N., Goldstein, I. J., Van Damme, E. J. M., and Peumans, W. J. (1988) Binding properties of a mannose-specific lectin from the snowdrop (Galanthus nivalis) bulb.J. Biol. Chem. 263, 728–734.
Yamamoto, K., Tsuji, T., Matsumoto, I., and Osawa, T. (1981) Structural requirements for the binding of oligosaccharides and glycopeptides to immobilized wheat germ agglutinin.Biochemistry 20, 5894–5899.
Ogata, S., Muramatsu, T., and Kobata, A. (1975) Fractionation of glycopeptides by affinity column chromatography on concanavalin A-Sepharose.J. Biochem. 78, 687–696.
Krusius, T., Finne, J., and Rauvala, H. (1976) The structural basis of the different affinities of two types of acidic N-glycosidic glycopeptides from concanavalin A-Sepharose.FEBS Lett. 71, 117–120.
Kornfeld, K., Reitman, M. L., and Kornfeld, R. (1981) The carbohydrate-binding specificity of pea and lentil lectins.J. Biol. Chem. 256, 6633–6640.
Katagiri, Y., Yamamoto, K., Tsuji, T., and Osawa, T. (1984) Structural requirements for the binding of glycopeptides to immobilizedvicia faba (fava) lectin.Carbohydr. Res. 129, 257–265.
Yamamoto, K., Tsuji, T., and Osawa, T. (1982) Requirement of the core structure of a complex-type glycopeptide for the binding to immobilized lentil-and pea-lectins.Carbohydr. Res. 110, 283–289.
Yamashita, K., Hitoi, A., and Kobata, A. (1983) Structural determinants ofPhaseolus vulgaris erythroagglutinating lectin for oligosaccharides.J. Biol. Chem. 258, 14,753–14,755.
Cummings, R. D. and Kornfeld, S. (1982) Characterization of the structural determinants required for the high affinity interaction of asparagine-linked oligosaccharides with immobilizedPhaseolus vulgaris leukoagglutinating and erythroagglutinating lectins.J. Biol. Chem. 257, 11,230–11,234.
Cummings, R. D. and Kornfeld, S. (1984) The distribution of repeating [Galβ1, 4GlcNAcβ1,3] sequences in asparagine-linked oligosaccharides of the mouse lymphoma cell line BW5147 and PHAR2.1.J. Biol. Chem. 259, 6253–6260.
Yamashita, K., Totani, K., Ohkura, T., Takasaki, S., Goldstein, I. J., and Kobata, A. (1987) Carbohydrate binding properties of complex-type oligosaccharides on immobilizedDatura stramonium lectin.J. Biol. Chem. 262, 1602–1607.
Irimura, T. and Nicolson, G. L. (1983) Interaction of pokeweed mitogen with poly(N-acetyllactosamine)-type carbohydrate chains.Carbohydr. Res. 120, 187–195.
Kawashima, H., Sueyoshi, S., Li, H., Yamamoto, K., and Osawa, T. (1990) Carbohydrate binding specificities of several poly-N-acetyllactosamine-binding lectins.Glycoconjugate J. 7, 323–334.
Wang, W.-C. and Cummings, R. D. (1988) The immobilized leukoagglutinin from the seeds ofMaackia amurensis binds with high affinity to complex-type Asn-linked oligosaccharides containing terminal sialic acid-linked α-2,3 to penultimate galactose residues.J. Biol. Chem. 263, 4576–4585.
Kawaguchi, T., Matsumoto, I., and Osawa, T. (1974) Studies on hemagglutinins fromMaackia amurensis seeds.J. Biol. Chem. 249, 2786–2792.
Sueyoshi, S., Yamamoto, K., and Osawa, T. (1988) Carbohydrate binding specificity of a beetle (Allomyrina dichotoma) lectin.J. Biochem. 103, 894–899.
Yamashita, K., Umetsu, K., Suzuki, T., Iwaki, Y., Endo, T., and Kobata, A. (1988) Carbohydrate binding specificity of immobilizedAllomyrina dichotoma lectin II.J. Biol. Chem. 263, 17,482–17,489.
Shibuya, N., Goldstein, I. J., Broekaert, W. F., Lubaki, M. N., Peeters, B., and Peumans W. J. (1987) Fractionation of sialylated oligosaccharides, glycopeptides, and glycoproteins on immobilized elderberry (Sambucus nigra L.) bark lectin.Arch. Biochem. Biophys. 254, 1–8.
Shibuya, N., Goldstein, I. J., Broekaert, W. F., Lubaki, M. N., Peeters, B., and Peumans, W. J. (1987) The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac(α2-6)Gal/GalNAc sequence.J. Biol. Chem. 262, 1596–1601.
Konami, Y., Yamamoto, K., Osawa, T., and Irimura, T. (1994) Strong affinity ofMaackia amurensis hemagglutinin (MAH) for sialic acid-containing Ser/Thr-linked carbohydrate chains of N-terminal octapeptides from human glycophorin A.FEBS Lett. 342, 334–338.
Osawa, T. and Tsuji, T. (1987) Fractionation and structural assessment of oligosaccharides and glycopeptides by use of immobilized lectins.Ann. Rev. Biochem. 56, 21–42.
Osawa, T. (1989) Recent progress in the application of plant lectins to glycoprotein chemistry.Pure & Appl. Chem. 61, 1283–1292.
Tsuji, T., Yamamoto, K., and Osawa, T. (1993) Affinity chromatography of oligosaccharides and glycopeptides with immobilized lectins, inMolecular Interactions in Bioseparations (Ngo, T. T., ed.), Plenum, New York, pp. 113–126.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamamoto, K., Tsuji, T. & Osawa, T. Analysis of asparagine-linked oligosaccharides by sequential lectin affinity chromatography. Mol Biotechnol 3, 25–36 (1995). https://doi.org/10.1007/BF02821332
Issue Date:
DOI: https://doi.org/10.1007/BF02821332