Abstract
The kinetics of phenol degradation was estimated in a fed-batch reactor system. Effects of oxygen and nutrient excess or limitation as well as the presence of several essential ions on the phenol- and oxygen-specific uptake rates achieved simultaneously in a bioreactor were shown.Candida tropicalis was grown on phenol as the only carbon and energy source. Applying the best fit of polynomial function, the maximum specific uptake rates of phenol and oxygen, the critical concentrations of phenol, the half-saturation constants and inhibition constants were determined. Linear relationship between specific phenol uptake rate and the exogenous respiration rate was found regardless of the kind and presence of essential nutrients. At oxygen limitation both the phenol uptake rate and the cell affinity to phenol decreased more strongly compared with those under nutrient limitation. Oxygen in excess resulted in a significant increase of cell tolerance toward phenol. The presence of essential nutrients increased the specific phenol degradation rate and led to complete phenol oxidation.
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Anselmo A.M., Cabral J.M.S., Novais J.M.: The adsorption ofFusarium flocciferum spores on cellite particles and their use in the degradation of phenol.Appl. Microbiol. Biotechnol.31, 200–203 (1989).
Arvin E., Jensen B.K., Gundersen A.T.: Biodegradation kinetics of phenols in a aerobic biofilm at low concentrations.Water Sci. Technol.23, 1375–1384 (1991).
Bechard G., Bisaillon J.G., Beaudet R.: Degradation of phenols by a bacterial consortium under methanogenic conditions.Can. J. Microbiol.36, 573–578 (1990).
Branyik T., Kuncová G., Páca J.: The use of silica gel prepared by sol-gel method and polyurethane foam as microbial carriers in the continuous degradation of phenol.Appl. Microbiol. Biotechnol.54, 168–172 (2000).
Chang Y.H., Li C.T., Chang M.C., Shieh W.K.: Phenol degradation byCandida tropicalis and its fusant.Biotechnol. Bioeng.60, 391–395 (1998).
Collins L.D., Daugulis A.J.: Characterization and optimization of a two-phase partitioning bioreactor for the biodegradation of phenol.Appl. Microbiol. Biotechnol.48, 18–22 (1997).
Dagley S.: Microbial metabolism of aromatic compounds pp. 483–505 in M. Moo-Young (Ed.):Comprehensive Biotechnology, Vol. 1. Pergamon Press, Oxford (UK) 1985.
Fan L.S., Fujie K., Long T.R., Tang W.T.: Characteristics of draft tube gas-liquid-solid fluidized-bed bioreactor with immobilized living cells for phenol degradation.Biotechnol. Bioeng.30, 498–504 (1987).
Folsom B.R., Chapman P.J., Pritchard P.H.: Phenol and trichloroethylene degradation byPseudomonas cepacia G4: kinetics and interactions between substrates.Appl Environ. Microbiol.56, 1279–1285 (1990).
Heipieper H.J., Keweloh H., Rehm H.J.: Influence of phenols on growth and membrane permeability of free and immobilizedEscherichia coli.Appl. Environ. Microbiol.57, 1213–1217 (1991).
Hensel J., Straube G.: Kinetic studies of phenol degradation byRhodococcus sp. P1—II. Continuous cultivation.Antonie van Leeuwenhoek57, 33–36 (1990).
Hill G.A., Robinson C.W.: Substrate inhibition kinetics: phenol degradation byPseudomonas putida.Biotechnol. Bioeng.17, 1599–1615 (1975).
Hobson M.J., Millis N.F.: Chemostat studies of a mixed culture growing on phenolics.Res. J. Water Pollut. Control Fed.62, 684–691 (1990).
Katayama-Hyraiama K., Tobita S., Hirayama K.: Metabolic pathway of phenol inRhodotorula rubra.J. Gen. Appl. Microbiol.37, 379–388 (1991a).
Katayama-Hirayama K., Tobita S., Hirayama K.: Degradation of phenol by yeastRhodotorula.J. Gen Appl. Microbiol.37, 147–156 (1991b).
Koturi G., Robinson C.W., Innis W.E.: Phenol degradation by a psychrotrophic strain ofPseudomonas putida.Appl. Microbiol. Biotechnol.34, 539–543 (1991).
Krug M., Ziegler H., Straube G.: Degradation of phenolics compounds by the yeastCandida tropicalis HP 15—I. Physiology of growth and substrate utilization.J. Basic Microbiol.25, 103–110 (1985).
Li J., Humphrey A.: Kinetic and fluorometric behaviour of a phenol fermentation, pp. 190–206 in T.K. Ghose (Ed.):Bioprocess Engineering. The First Generation. Ellis Horwood, Chichester (UK) 1989.
Limbert E.S.B., Betts W.B.: Kinetics of bio-oxidation of medium comparising phenol and mixture of organic contaminants.Appl. Microbiol. Biotechnol.43, 165–170 (1995).
Livingston A.G., Chase H.A.: Modeling phenol degradation in a fluidized-bed reactor.Am. Inst. Chem. Engin. J.35, 1980–1992 (1989).
Marek J., Páca J., Halecký M., Koutský B., Sobotka M., Keshavarz T.: Effect of pH and loading manner on the start-up period of peat biofilter degrading xylene and toluene mixture.Folia Microbiol.46, 205–209 (2001).
Martius G.G.S., Stottmeister U., Jechorek M., Páca J.: Inhibition concentration of phenolic substances under different cultivation conditions—part II: impact of dissolved oxygen concentration and temperature on degradation kinetics.Acta Hydrochim. Hydrobiol.24, 168–175 (1996).
Mason J.R.: The induction and repression of benzene and catechol oxidizing capacity ofPseudomonas putida ML2 studied in perturbed chemostat culture.Arch. Microbiol.162, 57–62 (1994).
Mörsen A., Rehm H.J.: Degradation of phenol by a defined mixed culture immobilized by adsorption on activated carbon and sintered glass.Appl. Microbiol. Biotechnol.33, 206–121 (1990).
Mörtberg M., Neujahr H.Y.:In situ andin vitro kinetics of phenol hydroxylase.Biochem. Biophys. Res. Commun.146, 41–46 (1987).
Müller R.H., Babel W.: Phenol and its derivatives as heterotrophic substrates for microbial growth—an energetic comparison.Appl. Microbiol. Biotechnol.42, 446–451 (1994).
Müller R.H., Babel W.: Growth rate-dependent expression of phenol-assimilation pathways inAlcaligenes eutrophus JMP 134—the influence of formate as an auxiliary energy source on phenol conversion characteristics.Appl. Microbiol. Biotechnol.46, 156–162 (1996).
Müller R.H., Bley T., Babel W.: Transient state cultivation as a means for determining maximum growth rates of microorganism in inhibition kinetics.J. Microbiol. Meth.22, 209–219 (1995).
Muñoz J.A., Pérez-Esteban B., Esteban M., de la Escalera S., Gómez M.A., Martínez-Toledo M.V., González-López J.: Growth of moderately halophilic bacteria isolated from sea water using phenol as the sole carbon source.Folia Microbiol.46, 297–302 (2001).
Okaygun M.S., Green L.A., Akgerman A.: Effects of consecutive pulsing of an inhibitory substrate on biodegradation kinetics.Environ. Sci. Technol.26, 1746–1752 (1992).
Páca J., Grégr V.: Method for the determination of oxygen transfer coefficients (KLa) with the correction for the actual cultivation conditions.J. Appl. Chem. Biotechnol.27, 155–164 (1977).
Páca J., Martius G.G.S.: Inhibition concentration of phenolic substances under different cultivation conditions—part I: phenol oxidation by mixed microbial population in a model system.Acta Hydrochim. Hydrobiol.24, 127–131 (1996).
Ruiz-Ordaz N., Hernandez-Manzano E., Ruiz-Lagunez J.C., Cristiani-Urbina E., Galindez-Mayer J.: Growth kinetic model that describes the inhibitory and lytic effect of phenol onCandida tropicalis yeast.Biotechnol. Progr.14, 966–969 (1998).
Sanchez J.L.G., Kamp B., Onysko K.A., Budman H., Robinson C.W.: Double inhibition model for degradation of phenol byPseudomonas putida Q5.Biotechnol. Bioeng.60, 560–568 (1998).
Seker S., Beyenal H., Salih B., Tanyolaç A.: Multi-substrate growth kinetic ofPseudomonas putida for phenol removal.Appl. Microbiol. Biotechnol.47, 610–614 (1997).
Shimizu T., Akitaya K., Fukuchi M., Nei N., Ichikawa K.: Basic decomposition parameters of phenol byCandida tropicalis.J. Ferment. Technol.51, 803–808 (1973a).
Shimizu T., Uno T., Dan Y., Nei N., Ichikawa K.: Continuous treatment of waste water containing phenol byCandida tropicalis.J. Ferment. Technol.51, 809–812 (1973b).
Spanning A., Neujahr H.Y.: Growth and enzyme synthesis during continuous culture ofTrichosporon cutaneum on phenol.Biotechnol. Bioeng.24, 464–468 (1987).
Spanning A., Neujahr H.Y.: The effect of glucose on enzyme activities and phenol utilization inTrichosporon cutaneum grown in continuous culture.J. Gen. Microbiol.136, 1491–1495 (1990).
Stephenson T.: Substrate inhibition of phenol oxidation by a strain ofCandida tropicalis.Biotechnol. Lett.12, 843–846 (1990).
Straube G., Hensel J., Niedan C., Straube E.: Kinetic studies of phenol degradation byRhodococcus sp. P1—I. Batch cultivation.Antonie van Leeuwenhoek57, 29–32 (1990).
Tang W.T., Fan L.S.: Steady state phenol degradation in a draft tube, gas-liquid-solid fluidized bed-biofilm reactor.Am. Inst. Chem. Engin. J.33, 239–247 (1987).
Watson-Craik I.A., Senior E.: Treatment of phenolic waste waters by co-disposal with refuse.Water Res.23, 1293–1303 (1989).
Weigner P., Páca J., Loskot P., Koutský B., Sobotka M.: The start-up period of styrene degrading biofilters.Folia Microbiol.46, 211–216 (2001).
Worden R.M., Donaldson T.L.: dynamics of a biological fixed film for phenol degradation in a fluidized bed reactor.Biotechnol. Bioeng.30, 398–405 (1987).
Yang R.D., Humphrey A.E.: Dynamic and steady-state studies of phenol biodegradation in pure and mixed cultures.Biotechnol. Bioeng.17, 1211–1235 (1975).
Zilly A., Souza C.G.M., Barbosa-Tessmann I.P., Peralta R.M.: Decolorization of industrial dyes by a Brazilian strain ofPleurotus pulmonarius producing laccase as the sole phenol-oxidizing enzyme.Folia Microbiol.47, 273–277 (2002).
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Páca, J., Komárková, E., Prell, A. et al. Kinetics of phenol oxidation byCandida tropicalis: Effects of oxygen supply rate and nutrients on phenol inhibition. Folia Microbiol 47, 701–707 (2002). https://doi.org/10.1007/BF02818675
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DOI: https://doi.org/10.1007/BF02818675