Abstract
All dried vegetative cells ofPithophora œdogonia died within 1 h, while those ofCladophora glomerata andRhizoclonium hieroglyphicum retain viability to some extent for 1 and 8 d, respectively, under similar storage conditions. The viability of dried vegetative cells of eitherC. glomerata orR. hieroglyphicum decreased more or less equally when stored either at 20 °C. in light or dark or at 12 °C in dark, but was lost rapidly and drastically when stored at 0 °C in dark. Both dried and wet akinetes ofP. œdogonia were equally more viable when stored at 20 °C in dark than in light, but they lost germination ability when stored either at 12 or 0 °C in dark; this might be either due to loss of viability or dormancy induction at low temperatures. The water stress imposed by growing vegetative filaments either on highly agarized media, in NaCl-supplemented liquid media or in media undergoing progressive air-drying to complete dryness did not induce, but reduced akinete formation inP. œdogonia, decreased zoosporangium formation inC. glomerata andR. hieroglyphicum, decreased or totally suppressed akinete germination inP. œdogonia and zoospore germination inC. glomerata andR. hieroglyphicum. Akinetes ofP. œdogonia formed under water stress were equally viable, while zoosporangia ofC. glomerata andR. hieroglyphicum formed under water stress were comparatively less viable than those formed without any water stress. Akinete germination inP. œdogonia and zoospore germination inC. glomerata andR. hieroglyphicum were comparatively more sensitive to water stress than the formation of akinetes and zoosporangia. The akinete germination inP. œdogonia was more sensitive to water stress than zoospore germination inC. glomerata andR. hieroglyphicum and it might be either due to their large size, thick wall or dense content.
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References
Agrawal S.C.: Effects of nutrients present in Bold's basal medium on sporulation of the green algaPithophora œdogonia (Mont.) Wittrock.Microbios Lett. 24, 27–29 (1983).
Belcher J.H.: The resistance of desiccation and heat of the asexual cysts of some freshwaterPrasinophyceae.Brit. Phycol. J. 5, 173–177 (1970).
Cameron R.E., Blank G.R.: Desert algae: soil crusts and diaphanous substrata as algal habitats.Jet Propulsion Laboratory Pasadena Tech. Rep. No. 32-971, pp. 1–45. Pasadena (USA) 1966.
Evans J.H.: The survival of freshwater algae during dry periods. I. An investigation of the algae of five small ponds.J. Ecol. 46, 149–168 (1958).
Fritsch F.E.: The morphology and ecology of an extreme terrestrial form ofZygnema ericetorum (Kutz.) Hass.Ann. Bot. 30, 135–149 (1916).
Holm-Hansen O.: Viability of lyophilized algae.Can. J. Bot. 42, 127–137 (1964).
Hwang S.W., Horneland W.: Survival of algal culture after freezing by controlled and uncontrolled cooling.Cryobiology 1, 305–311 (1965).
Lembi C.A., Spencer D.F.: Role of the akinete in the survival ofPithophora (Chlorophyceae).J. Phycol. 17 (Suppl.), 8 (1981).
Lippert B.E.: Sexual reproduction inClosterium moniliferum andClosterium ehrenbergii.J. Phycol. 3, 182–198 (1967).
Metzner J.: A morphological and cytological study of new form ofVolvox.Bull. Torrey Bot. Club 72, 86–136 (1945).
Moewus F.: Die Vererbung des Geschlechts bei Verschiedenen Rassen vonProtosiphon botryoides.Arch. Protistenkd.86, 1–57 (1935).
Nichols H.W., Bold H.C.:Trichosarcina polymorpha gen. et sp. nov.J. Phycol. 1, 34–38 (1965).
Proctor V.W.: Storage and germination ofChara oospores.J. Phycol. 3, 90–92 (1967).
Sili C., Ena A., Materassi R., Vincenzini: Germination of desiccated aged akinetes of alkaliphilic cyanobacteria.Arch. Microbiol. 162, 20–25 (1994).
Stahl E.: Über die Ruhezustande derVaucheria geminata.Bot. Zeit. 37, 129–137 (1879).
Starr R.C.: Reproduction by zoospores inTetraedron bitridens.Am. J. Bot. 41, 17–20 (1954).
Watanabe A.: Some devices for preserving blue-green algae in viable state.J. Gen. Appl. Microbiol. 5, 153–157 (1959).
Whitton B.A.: Effect of deep-freeze treatment on blue-green algal cultures.Brit. Phycol. Bull. 2, 177–178 (1962).
Yamamoto Y.: Effect of desiccation on the germination of akinetes ofAnabœna cylindrica.Plant Cell Physiol. 16, 749–752. (1975).
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Agrawal, S.C., Singh, V. Viability of dried vegetative cells and the formation and germination of reproductive structures inPithophora œdogonia, Cladophora glomerata andRhizoclonium hieroglyphicum under water stress. Folia Microbiol 44, 63–70 (1999). https://doi.org/10.1007/BF02816224
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DOI: https://doi.org/10.1007/BF02816224