Abstract
ATrichoderma sp. isolate, hereafter called T6, produces a 46-kDa endochitinase (CHIT 46) which had been shown to drastically affectin vitro the cell walls of the phytopathogensSclerotium rolfsii andRhizoctonia solani. We attempted to gain insight into its properties. The CHIT 46 N-terminal amino acid sequence shares a very high homology with other fungal chitinases. Western blot analysis using polyclonal antibodies anti-CHIT 46 revealed that this enzyme is immunologically distinct from other proteins produced by the sameTrichoderma isolate T6, but is immunologically identical with proteins having equivalent molar mass, probably chitinases, produced by otherTrichoderma spp. isolates. In addition, the antibodies revealed also that a substantial amount of this enzyme is secreted into the culture medium 2 d after theTrichoderma isolate T6 comes into contact with chitin.
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Lima, L.H.C., De Marco, J.L., Ulhoa, C.J. et al. Synthesis of aTrichoderma chitinase which affects theSclerotium rolfsii andRhizoctonia solani cell walls. Folia Microbiol 44, 45–49 (1999). https://doi.org/10.1007/BF02816220
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DOI: https://doi.org/10.1007/BF02816220