Abstract
The growth and methane formation ofMethanospirillum hungatei were inhibited by an inhibitor of Na+/H+ antiport amiloride. After addition of NaCl or LiCl, when the cells had a lower intracellular pH and were deenergized, they extruded protons into the external medium. The acidification of the external medium was stimulated by protonophores and inhibited by amiloride. These findings suggest the existence of an Na+/H+ antiport in the cytoplasmic membrane ofM. hungatei and its role in the energetics of methanogenic bacteria.
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Rusňák, P., Šmigáň, P. & Greksák, M. Evidence for Na+/H+ antiport inMethanospirillum Hungatei . Folia Microbiol 37, 12–16 (1992). https://doi.org/10.1007/BF02814573
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DOI: https://doi.org/10.1007/BF02814573