Abstract
The culture parameters required for optimum production of extracellular α-amylase by the thermophilicBacillus coagulans are described. The optimum pH, temperature and incubation period for amylase production were 7, 50°C and 48 h, respectively. Age of inoculum (48 h) and its level, (2%) were critical for maximum amylase yield. The enzyme secretion was high in rice starch and beef extract as compared to other carbon and nitrogen sources tested. The addition of mustard oil cake (1%) and agitation at 1.7 Hz resulted in an enhancement of α-amylase secretion.
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Babu, K.R., Satyanarayana, T. Parametric optimization of extracellular α-amylase production by thermophilicBacillus coagulans . Folia Microbiol 38, 77–80 (1993). https://doi.org/10.1007/BF02814555
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DOI: https://doi.org/10.1007/BF02814555