Abstract
This paper describes cell-free enzymatic syntheses of sucrose and trehalose using partially-purified preparations of sucrose and trehalose synthetase. The coupling of the regeneration of uridine-5′-diphosphoglucose (UDP-Glc) with synthesis of the disaccharide offers a practical route to millimol quantities of these carbohydrates. The syntheses used pyruvate kinase, UDP-Glc pyrophosphorylase, and inorganic pyrophosphatase, and the regenerated UDP-Glc was cycled approximately 10 times.
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This work was supported by the NIH through several grants, most recently GM 30367.
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Haynie, S.L., Whitesides, G.M. Enzyme-catalyzed organic synthesis of sucrose and trehalose with in situ regeneration of UDP-Glucose. Appl Biochem Biotechnol 23, 155–170 (1990). https://doi.org/10.1007/BF02798384
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DOI: https://doi.org/10.1007/BF02798384