Abstract
Various catalytically active antibodies (Abs), or abzymes, have been detected recently in the sera of patients with autoimmune pathologies, in whom their presence is probably associated with autoimmunization. Normal humans are generally not considered to have abzymes, since no obvious immunizing factors are present. Here is shown by different methods that IgG from the milk of normal females possesses both DNase and RNase activities. The activities were also present in the IgG F(ab′)2 and Fab fragments.
Affinity modification of IgG by the chemically reactive derivative of an oligonucleotide led to preferential modification of the L chain of IgG. After separation of the subunits by sodium dodecyl sulfate electrophoresis in a gel containing DNA, an in-gel assay showed DNase activity in the L chain. The L chain separated by affinity chromatography on DNA-cellulose was catalytically active. These findings speak in favor of the generation of cat alytic Abs by the immune system of healthy mothers. It is known that the treatment of adults with DNases and RNases offers protection from viral and bacterial diseases. Since breast milk protects the infants from infec tions until the immune system is developed, this raises the possibility that catalytic Abs like nucleases, may possess a protective role.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- Abs:
-
antibodies
- slgA:
-
secretory IgA
- PAGE:
-
polyacrylamide gel electrophoresis
References
Tramontano, A., Janda, K. D., and Lerner, R. A. (1986),Science 234, 1566–1569.
Lerner, R. A., and Tramontano, A. (1981),TIBS 12, 427–438.
Lerner, R. A., Benkovic, S. J., and Shultz, P. J. (1991),Science 252, 659–667.
Benkovic, S. J. (1992),Annu. Rev. Biochem. 61, 29–54.
Hilvert, D. (1992),Pyre Appl. Chem. 64, 1103–1113.
Suzuki, H. (1994),J. Biochem. 115, 623–628.
Reimer, C. Raska, L., Tan, E. M., and Sheer, U. (1987),Virchows Arch. 54, 131–136.
Earnshow, W. C., and Rothfield, N. (1985),Chromosoma 91, 313–320.
Paul, S., Volle, D. J., Beach, C. M., Johnson, D. R., Powell, M. J., and Massey, R. J. (1989),Science 244, 1158–1161.
Shuster, A. M., Gololobov, G. V., Kvashuk, O. A., Bogomolova, A. E., Smirnov, I. V., and Gabibov, A. G. (1992),Science 256, 665–667.
Buneva, V. N., Andrievskaya, O. A., Romannikova, I. V., Gololobov, G. V., Yadav, R. P., Yamkovoi, V. L., and Nevinsky, G. A. (1994),Mol. Biol. 28, 483–486.
Kit, Yu. Ya., Semenov, D. V., and Nevinsky, G. A. (1995),Mol. Biol. 29, 519–526.
Saif, L. J. and Bohl, E. H. (1979), inImmunology of Breast Milk, Ogra, P. L. and Dayton, D. H., ed., Raven, New York, pp. 237–255.
Redhead, K., Hill, T., and Mullroy, B. (1990),FEMS Microbiol. Lett. 70, 269–274.
Gillin, F. D., Reiner, D. S., and Wang, C. S. (1983),Science 221, 1290–1292.
Fiat, A.-M., and Jolies, P. (1989),Mol. Cell. Biochem. 87, 5–9.
Brambell, F. W. (1970), inThe Transmission of Passive Immunity from Mother to Young. Neuberger, A. and Tatum E. L., ed., North-Holland, Amsterdam and London.
Shapot, V. S. (1968), inNucleases, Medicine Press, Moscow, pp. 7–125.
Glukhov, B. M., Ierusalimskii, R. P., and Salganik R. I. (1968)J. Neurol. Psychiatr. (Russian)10, 361–368.
Mashkovsky, M. D. (1984) inMedical Preparations, Medicine Press, Moscow,2, 53–54.
Lobzin, V. S., and Sichko, J. V. (1969)Medical Approaches (Vrachebnoe delo),10, 38–41.
Ramaswamy, H., Swamy, Ch. V. B., and Das, M. R. (1993),J. Biol. Chem. 268, 4181–4187.
Friemel, F. (1984), inImmunologische Arbeitsmethogen. Veb Gustav Fisher Verlag Jena.
Towbin H., Staehein T., and Gordon J. (1979)Proc. Natl. Acad. Sci. USA,76, 4350–4354.
Gololobov, G. V., Chernova, E. A., Schourov, D. V., Smirnov, I. V., Kudelina, I. A., and Gabibov, A. G. (1995),Proc. Natl. Acad. Sci. USA 92, 254–257.
Milligan, J. F., Groebe, D. R., Witherell, G. W., and Uhlenbeck, O. C. (1987),Nucleic Acids Res. 15, 8783–8798.
Vlassov, V. V., Giege, R., and Ebel, J.-P. (1981)Eur. J. Bioch. 119, 51–59.
Vlassov, V. V., Zuber, G., Felden, B., Behr, J.-R, and Giege, R. (1995),Nucl. Acids Res. 23, 3161–3167.
Buneva, V. N., Godovikova, T. S., and Zarytova, V. F. (1986),Bioorgan. Chemistry (Russian) 12, 906–910.
Rosenthal, A. L. and Lacks, S. A. (1977),Anal. Biochem. 80, 76–82.
Harlow, E. and Lane, D. (1988), inAntibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 630–631.
Lee, J. S., Dombrovskii, D. R., and Mosmann, T. (1982)Biochemistry 21, 4940–4947.
Cathou, R. E. (1978), inImmunoglobulins, Litman, K. W., and Good, R. A., ed., New York: Plenum, p. 100.
Nevinsky, G. A., Ankilova, V. N., Lavrik, O. I., Mkrtchyan, Z. S., Nersesova, L. S., and Akopyan J. I. (1982),FEBS Lett. 149, 36–40.
Andrievskaya, O. A., Buneva, V. N., Yamkovoi, V. I., and Nevinskii, G. A. (1997),Dokl. Ross. Akad. Nauk. (Russia) 355, 401–303.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Buneva, V.N., Kanyshkova, T.G., Vlassov, A.V. et al. Catalytic DNA-and RNA-hydrolyzing antibodies from milk of healthy human mothers. Appl Biochem Biotechnol 75, 63–76 (1998). https://doi.org/10.1007/BF02787709
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02787709